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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, meets national standard methods, limited documentation, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: AFNOR (draft, not further specified)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Pyrene
EC Number:
204-927-3
EC Name:
Pyrene
Cas Number:
129-00-0
Molecular formula:
C16H10
IUPAC Name:
pyrene
Details on test material:
- Name of test material (as cited in study report): pyrene
- Molecular formula (if other than submission substance): C16H10
- Molecular weight (if other than submission substance): 202
- Substance type: organic
- Physical state: solid
- Source: no data
- Analytical purity: no data
- Typical concentration in coal-tar pitch: 0.94 - 1.48 % (data from NL 2008: Coal-Tar Pitch, Annex-XV dossier )
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES (data from NL 2008: Coal-Tar Pitch, Annex-XV dossier, and WHO 1998: EHC 202, if not otherwise indicated)
- Vapour pressure: 0.6 - 1.0*10^-3 Pa (at 25 °C)
- Water solubility: in freshwater, approx. 125 - 135 µg/L (20 - 25 °C);
soluble portion of the WSF of coal-tar pitch (loading 100 mg/L): 158 - 326 ng/L (mean 240 ng/L, n = 5)
[see 4.8: NOACK 2009_WSF, 100 mg/L nominal]
- Henry's law constant: 1.4 Pa*m3/mol (at 25 °C)
- log Pow: = 5.0 - 5.2

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
no data

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution close to water saturation used for dilution series (no detailed data, see Report p. 34)
- Eluate: no
- Differential loading: no
- Controls: with and without vehicle
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no data
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): no data

Test organisms

Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Source: INERIS
- Age of parental stock (mean and range, SD): <24 h
- Feeding during test
- Food type: Chlorella vulgaris / Pseudokirchneriella subcapitata / fish diet
- Offspring: Males were counted and removed from the test medium.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
7 d

Test conditions

Hardness:
240 mg/L (as CaCO3)
Test temperature:
25 +- 2°C
pH:
8.1 +-0.4
Dissolved oxygen:
>7.0 mg/L
Nominal and measured concentrations:
Nominal and analytical values not documented.
Analytical: calculation of the geometric mean from measured concentrations in fresh and spent medium,
based on an arithmetic model (OECD 1999) (see Report, p. 44/45).
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed (screw cap)
- Material, size, headspace, fill volume: glass vessel (90 mL), test volume 50 mL
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): 1x/d, 5x/wk (each workday)
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): none


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural mineral water (Evian)
- Ca/Mg ratio: approx. 3:1
- Conductivity: 460 +-100 µs/cm


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
- Light intensity: 500 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality adults and neonates, abortions, reproduction


VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations: no data
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
7 d
Dose descriptor:
EC10
Effect conc.:
2.1 µg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
dissolved
Remarks:
test material
Basis for effect:
other: mortality and reproduction
Duration:
7 d
Dose descriptor:
EC10
Effect conc.:
2.1 µg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Remarks:
test material
Basis for effect:
other: mortality and reproduction
Remarks on result:
other: 95% C.I.: 1.3 - 3.1 µg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes