N-(4-{[3-(dimethylamino)propyl]sulfamoyl}phenyl)-2-[(2-methoxy-4-nitrophenyl)diazenyl]-3-oxobutanamide

Water solubility

Administrative data

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13-18.03.2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP - Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

column elution method

Test material

Test material information

Constituent 1

Reference substance name:

N-(4-{[3-(dimethylamino)propyl]sulfamoyl}phenyl)-2-[(2-methoxy-4-nitrophenyl)diazenyl]-3-oxobutanamide

EC Number:

700-222-4

Cas Number:

1065519-44-9

Molecular formula:

C22H28N6O7S

IUPAC Name:

N-(4-{[3-(dimethylamino)propyl]sulfamoyl}phenyl)-2-[(2-methoxy-4-nitrophenyl)diazenyl]-3-oxobutanamide

Details on test material:

- Name of test material (as cited in study report): No. 408 Yellow
- Physical state: Yellow powder
- Analytical purity: 98.6%
- Lot/batch No.: 081105-1
- Expiration date of the lot/batch: 04.11.2009
- Stability under test conditions: stable
- Storage condition of test material: At room temperature in the dark

Results and discussion

Water solubility

Water solubility:

ca. 19.8 µg/L

Temp.:

20.3 °C

pH:

>= 7.1 - <= 7.2

Details on results:

The results for the samples taken at a flow rate of 12 and 24 ml/h are given in Table 1. The water solubility is given as a mean value of the measurements at both flow rates, because the coefficient of variation for both flow rates (CV) (n=10) and maximum difference on the water solubility was <30%. No undissolved particles were present in eluates.
The results of the accuracy samples are shown in Table 2.
The mean accuracy (value above the range 70-110%) was used to correct the water solubility data present in Table 1.

Any other information on results incl. tables

Table 1:

Flow rate [ml/h]	Sample no.	Concentration analysed				pH
		Individual [mg/l]	Mean 1 [mg/l] at one flow rate	Mean 2 [mg/l] at one flow rate corrected for the mean recovery of the procedural recovery samples (113%)	CV [%]
24	1	0.0196	0.0231	0.0204	15	7.1
	2	0.0203
	3	0.0214
	4	0.0203
	5	0.0191
	6	0.0235
	7	0.0256
	8	0.0291
	9	0.0281
	10	0.0239
12	1	0.0249	0.0217	0.0192	12	7.2
	2	0.0196
	3	0.0208
	4	0.0194
	5	0.0249
	6	0.0212
	7	0.0176
	8	0.0236
	9	0.0208
	10	0.0248
Mean of all concentrations, corrected for the mean recovery of the procedural recovery samples (113%): 0.0198 mg/l, MD: 6%

Table 2:

Target concentration [μg/l]	Nominal concentration [μg/l]	Analysed concentration [μg/l]	Accuracy [%]	Mean accuracy [%]
22	22.1	24.9 25.1	113 114	113