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EC number: 230-029-6 | CAS number: 6920-22-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15-Jun, 1998 to 6-August, 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline test with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- DL-hexane-1,2-diol
- EC Number:
- 230-029-6
- EC Name:
- DL-hexane-1,2-diol
- Cas Number:
- 6920-22-5
- Molecular formula:
- C6H14O2
- IUPAC Name:
- hexane-1,2-diol
- Reference substance name:
- 1,2-hexanediol
- IUPAC Name:
- 1,2-hexanediol
- Test material form:
- other: liquid
Constituent 1
Constituent 2
Method
- Target gene:
- S. typhimurium histidine (his) reversion system measures his- → his+ reversions.
E. coli WP2 and E.coli wp2 uvr A pKM101
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- S. typhimurium TA 1538
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- E. coli WP2 uvr A pKM 101
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver s9
- Test concentrations with justification for top dose:
- the preliminary toxicity assay: 6.7, 10, 33, 67, 100,333, 667, 1000, 3333, 5000 µg/plate;
the mutagenicity assays: 25, 75, 200, 600, 1800, 5000 µg/plate - Vehicle / solvent:
- water was selected as the solvent of choice based on solubility of the test article and compatibility with the target cell.
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- with s9
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- 1.0 µg/plate for all salmonella strains
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- with s9
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- 10 µg/plate for WP2 uvrA (pKM101)
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- with s9
- Positive control substance:
- other: sterigmatocystin
- Remarks:
- 100 µg/plate for WP2(pKM)101
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- without s9
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- 1.0 µg/plate for TA98, TA 1538
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- without s9
- Positive control substance:
- sodium azide
- Remarks:
- 1.0 µg/plate for TA100, TA1535
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- without s9
- Positive control substance:
- other: 9-aminoacridine
- Remarks:
- 75 µg/plate for TA1537
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- without s9
- Positive control substance:
- methylmethanesulfonate
- Remarks:
- 1000 µg/plate for Both E.coli strains
- Details on test system and experimental conditions:
- Test system:
The tester strains used were the salmonella typhimurium histidine auxotrophs TA 98, TA 100, TA 100, TA 1537 and TA 1538 as described by Ames etal.(1975) and Escherichia cloi tester strains WP2 uvrA (PKM 101) and WP2(PKM 101). Salmonella tester strains were received on 11/10/92 directly from Dr. Bruce Ames, University of California, Berkeley. E.coli was received on 07/01/87 and 02/19/93 from the National Collection of Industrial and Marine Bacteria, Aberdeen, Scotland.
Tester strains TA 98, TA 1537 and TA 1538 are reverted from histidine dependence(auxotrophy) to histidine independence(prototrophy) by frameshift mutagens. Tester strain TA 1535 is reverted by mutagens that cause basepair substitutions. Tester strain TA100 is reverted by mutagens that cause both frameshift and basepair substitution mutations. Specificity of the reversion mechanism in E.coli is sensitive to base-pair substitution mutations, rather than frameshift mutations.
Metabolic activation system
Aroclor 1254-induced rat liver s9 was used as the metabolic activation system. The s9 was prepared from male sprague-Dawley rats induced with a single intraperitoneal injection of Aroclor 1254, 500mg/kg, five days prior to sacrifice. The s9 was batch prepared on 02/25/98 and 02/26/98 and stored at <=-70 degree until used. Each bulk preparation of s9 was assayed for its ability to metabolized 2-aminoanthracene and 7,12-dimethylbenz(a)anthracene to forms mutagenic to Salmonella typhimurium TA 100. - Evaluation criteria:
- For the test article to be evaluated positive, it must cause a dose-related increase in the mean revertants per plate of at least one tester strain with a minimum of two increasing concentrations of test article. Data sets ofr strains TA1535, TA1537 and TA1538 were judged positive in the increase in mean revertants at the peak of the dose response is equal to or greater than three times the mean vehicle control value. Data sets for strains TA98, TA100 and WP2 uvrA(pKM101) and WP2 (pKM101) were judged positive if the increase in mean revertants at the peak of the dose response is equal or greater than two times the mean vehicle control value.
- Statistics:
- Revertant colonies for a given tester strain and activation condition, except for positive controls, were counted either entirely by automated colony counter or entirely by hand. For each replicate plating, the mean and standard deviation of the number of revertants per plate were calculated and are reported.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- other: TA 98, TA100, TA1535, TA1537 and TA1538
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- toxicity was observed at the 5000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- other: TA 98, TA100, TA1535, TA1537 and TA1538
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- other: E. coli WP2 uvr A pKM 101 and E. coli WP2 pKM 101
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- slubility test:
This test was conducted with the vehicle. The test article was soluble in water at approximately 500 mg/ml, the maximum concentration tested.
Priliminary toxicty assay
In the preliminary toxicity assay, the maximum dose tested was 5000 µg/plate; this dose was achieved using a concentration of 100 mg/ml and a 50 µl plating aliquot. No precipitate was observed but toxicity was generally observed at 5000 µg/plate with tester strains TA 98, TA100, TA1535, TA1537 and TA1538 in the absence of s9 only. Based on the findings of the toxicity assay, the maximum dosed plated in the mutagenicity assay was 5000 µg/plate.
In Experiment B1, the initial mutagenicity assay, no positive responses were observed with any of the tester strains in the presence of s9 and with tester strains TA1538,WP2uvrA(pKM101) and WP2pKM 101 in the absence of s9.
In experiment B2, no positive responses were observed with tester strains TA1535 and TA1537 without s9.
In experiment B3,no positive responses were observed with tester strain TA98 without s9.
In experiment B4, no positive responses were observed with tester strains TA98, TA100, TA1535, TA1537 and WP2 (pKM101) with s9. and with TA98, TA100, TA1535, TA1537 without s9.
In experiment B5, no positive responses were observed with tester strains TA1538, WP2uvrA(pKM101) and WP2pKM 101 with and without s9. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
The results of the Salmonella/Mammalian Microsome (Ames test) and Escherichia coli WP2 mutagenesis assay indicate that test article did not cause a positive respone with any of the tester strains with and without metabolic activation under the conditions of this study. - Executive summary:
This study was conducted following OECD guideline 471 with GLP regulations. Test article was tested in the bacterial reverse mutation assay using S.typhimurium tester strains TA98, TA100, TA 1535, TA 1537 TA1538 E.coli tester strains WP2 uvrA (pKM101) and WP2 (pKM101) in the presence and absence of Aroclor-induced rat liver S9. The assay was performed in two phases, using the plate incorporation and preincubation methods.In the preliminary toxicity assay, test article was tested at levels of 6.7, 10, 33, 67, 100, 333, 667, 1000, 3333, 5000 µg/plate. Based on the results of preliminary test, the mutagenicity assays was conducted at dose levels of 25, 75, 200, 600, 1800, 5000 µg/plate. All dose levels of test article and controls were plated in triplicate. All criteria for a valid study were met.
Based on the results of mutagenesis assay, test article caused negative response with any of the tester strains in the absence and presence of metabolic activation under the conditions of this study.
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