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EC number: 227-774-4 | CAS number: 5977-14-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well performed OECD study with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- not specified
- GLP compliance:
- yes
Test material
- Reference substance name:
- Acetoacetamide
- EC Number:
- 227-774-4
- EC Name:
- Acetoacetamide
- Cas Number:
- 5977-14-0
- Molecular formula:
- C4H7NO2
- IUPAC Name:
- acetoacetamide
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Brachydanio rerio
- Strain:Hamilton-Buchanan
- Source: West Aquarium, 3422 Bad Lauterberg
- Age at study initiation: about 3 months
- Length at study initiation: 2.5 - 3.5 cm average: 3 cm
- Feeding during test: twice a day ad libitum
- Food type: Tetra Min, Tetra Werke. Melle
ACCLIMATION
- Acclimation period: at least 1 days before start of the tests
- Acclimation conditions: same as test:
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- no
Test conditions
- Test temperature:
- 21.2 - 23.0 °C
- pH:
- 7.7 - 8.7
- Dissolved oxygen:
- about 9 mg/L
- Nominal and measured concentrations:
- Nominal: 5000 mg/L measured (average) 4985.7 mg/L
Nominal: 10000 mg/L measured (average) 10098 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:open Glass bassin (30 x 22 x 24 cm), calibrated for 10 Liters, standing in a waterbath made from plastics.
The temperature of the water bath was controlled for 22+-1 °C by a thermostate
- Aeration: no aeration except the 1000 mg/L group after 48 hours
OTHER TEST CONDITIONS
- Photoperiod: day / night: 12 h / 12 h
- Light intensity: 700 Lux - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 500 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 0 from 30 fish
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 5 000 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 0 from 10 fish
- Duration:
- 48 h
- Dose descriptor:
- other: LC60
- Effect conc.:
- 10 000 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 6 from 10 fish
- Duration:
- 96 h
- Dose descriptor:
- other: LC80
- Effect conc.:
- 10 000 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 8 from 10 fish
- Details on results:
- - Behavioural abnormalities: increased breathing, increased or decreade activity, swim posture tail-heavy,
Integument dark colored
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- For the 30% acetoacetamid solution the LC50 was determined as 5000 to 10000 mg/L and LC0=5000 mg/L.
This means, for the pure substance these values can be calculated as
LC0 = 1500 mg/L (pure substance) and
LC50 = 1500 - 3000 mg/L (pure substance)
Therefore acetoacetamid is not sublect for classification concerning the toxicity to fish - Executive summary:
A 30 % solution of acetoacetamide was tested for toxicity to zebra fish acc. OECD 203 guideline. Nominal concentrations of 500 mg/L, 5000 ml/L and 10000 mg/L were used. After 96 hours no mortality was observed up to 5000 mg/L. For 10000 mg/L after 48 hours 6 of 10 fish have died and after 96 8 of 10 fish. For LC 50 only a range can be given as LC50 = 5000 to 10000 mg/L and for
the LC0=5000 mg/L
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