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Diss Factsheets
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EC number: 235-101-0 | CAS number: 12068-53-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 Mar - 16 Mar 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- adopted 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- adopted 2008
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Mainz, Germany
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples were taken from the control and the 100 mg/L treatment at 0 and 96 h.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: As solubility lies below 100 mg/L, a saturated solution was prepared for the test. This was done by weighing the nominal load, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45 µm filters. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Source: The original animals were obtained from Umweltbundesamt (UBA) Berlin, Germany and used for breeding and production of eggs.
- Age at study initiation (mean and range, SD): sexually immature young fish
- Length at study initiation: 2 ± 1 cm
- Feeding during test: none
ANIMAL HUSBANDRY
- Culture vessels: polyethylene aquaria
- Culture medium: dechlorinated tap water
- Type and amount of food: warm-water fish food and daphnia, approx. 1 - 2% of body weight per day
- Feeding frequency: three times per day until 24 h before test start
- Medium renewal: twice per week
- Light and temperature: 12 h light/12 h dark, 28 °C - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 1.08 mmol/L
- Test temperature:
- 21.5 - 23.2 °C
- pH:
- 7.6 - 8.1
- Dissolved oxygen:
- 7.7 - 8.8 mg/L
- Nominal and measured concentrations:
- Nominal: control and 100 mg/L
Measured concentrations of zinc at 0 h: control 0.058 mg/L, treatment 0.038 mg/L
Measured concentrations of zinc after 96 h: control 0.025 mg/L, treatment 0.030 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: aquaria
- Material, size, fill volume: glass, 10 L, 7 L
- Aeration: frequency of bubbles 1/second
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: good quality drinking water
- Total organic carbon: 0.5 mg/L
- Metals (mg/L): Na 5.2, K 2.6, Ca 35.7, Mg 4.6, Al 0.007, Fe/Mn/Sb/As/Pb/Cd/Cr/Cu/Ni/Hg/Se < 0.005
- Pesticides: < LOD
- Chloride: 12 mg/L
- Ca/Mg ratio: 7.76:1
- Conductivity: 223 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: Oxygen concentration and pH were measured every 24 h.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12 h light/12 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Mortality and abnormal behaviour were observed every 24 h. - Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: filtered test solution
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: No abnormalities were observed in the control and test vessels.
- Mortality of control: No mortality was observed in the control.
- Effect concentrations exceeding solubility of substance in test medium: yes
- All validity criteria are met.
- Stability of test item solutions: After 96 h 94% of the initial zinc amount were measured, hence the standard in Danio test medium showed sufficient stability for zinc content over the test period.
Reference
Description of key information
LC50 (96h) > 100 mg/L for Danio rerio (OECD 203)
Key value for chemical safety assessment
Additional information
One study on fish toxicity is available for Zinc aluminium oxide. This key study was performed as a limit test according to OECD Guideline 203, in a static system, using Danio rerio as test organism (2012). As the solubility of the test substance lies below 100 mg/L, a saturated solution was prepared for the test. This was done by weighing the nominal load, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45 µm filters. The fish were exposed to the filtered test solution for 96 hours. As no mortalities were observed during the test, the LC50 was determined to be > 100 mg/L. Measured Zn concentrations in control and test solution were in the same range (control: 0.058 - 0.03 mg Zn/L, 100 mg/L: 0.03 - 0.038 mg Zn /L).
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