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EC number: 500-233-2 | CAS number: 68891-29-2 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Feb 13 - Mar 06, 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- 3 October, 2008
- Deviations:
- yes
- Remarks:
- The pH values (7.77– 9.91) outside recommended range of 6 – 9, deviation > 1.5 units at all treatment levels. The temperature was 18.1 – 20.9°C, variance < 2°C was not maintained. This had no effect on reproduction or the test concentrations.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations
- Sampling method: sampled from representative fresh (0 h) and aged (24 h) test solutions three times per week from pooled solutions
- Sample storage conditions before analysis: immediately analysed or stored in a freezer at -25°C until analysis - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was directly dissolved with 2 hours stirring. An aliquot of the test item (purity 73.3%) was weighed onto a sterile Teflon plate which was then placed directly into a sterile 2 L Schott bottle. Under laminar flow 2 L of sterile dilution water was added to the mixing vessels to reach a loading corresponding to the highest test concentration of 12 mg a.s./L.
The content of the vessel was stirred at 300 rpm for 2 h at room temperature (21 ± 1°C). The eluate was then used to begin a 50% serial dilution for the preparation of the remaining four test concentrations using sterilised dilution water.
The eluate was prepared 2 h under sterile conditions and replaced daily. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: German Federal Environment Agency, Institut für Wasser-, Boden- und Lufthygiene. Specimens used in the test were bred in the laboratory of the Fraunhofer IME
- Age of parental daphnids: at least 3 weeks old
- Feeding during test: yes
- Food type: with concentrated suspensions of unicellular alga Desmodesmus subspicatus
- Amount: of 0.2 mg carbon/L per daphnid per day
- Frequency: at each media renewal (daily)
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: . Newborn Daphnia (between 4 and 23 hours old) were removed by wide-bore pipette (to avoid damage) and isolated in fresh dilution water for at least 1 hour prior to being added randomly to the test vessels containing the appropriate test or control media. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 1.0 mmol/L
- Test temperature:
- 18.1 - 20.9 °C
- pH:
- 7.77 - 9.91
- Dissolved oxygen:
- 6.8 - 11.0 mg/L
- Nominal and measured concentrations:
- Nominal test concentrations: control, 0.51, 1.13, 2.48, 5.45, 12 mg/L
Mean measured test concentrations (TWA): -, 0.45, 1.03, 2.33, 5.27, 11.75 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Material, nominal volume, fill volume: glass, 100 ml, 60 ml
- Aeration: no
- Renewal rate of test solution: daily
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Cu-free tap water according to OECD guideline 211 sourced from the Schmallenberg district water production plants, mostly fed by small springs and percolation. The purification process occurs on site at Fraunhofer IME and includes filtration with activated charcoal, passage through a lime-stone column, and aeration to the point of oxygen saturation.
- Alkalinity: 1.3-1.5 mmol/L
- Conductivity: 203.7 - 227.9 µS/cm
- Culture medium different from test medium:- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Photoperiod: light/dark cycle of 16/8 hours
- Light intensity: 800 lx
EFFECT PARAMETERS MEASURED: The numbers of immobile daphnids were visually determined daily and the immobile daphnids were removed. Any abnormalities in appearance and behaviour were recorded. The newborn daphnids per beaker were counted and removed daily until day 10 (after day 10 newborn daphnids were counted and removed daily at each water renewal), abnormalities in condition (including male sex) or presence of winter eggs were checked and recorded. At study termination, length of the adults up to the highest treatment without significant mortality were measured by digital photography and image analysis and compared with the equally measured control animals.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2 - Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 5.3 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 11.75 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 11.75 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Remarks:
- parental daphnids
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 11.75 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth
- Remarks:
- (body length)
- Details on results:
- - Mortality of parent animals: One parental daphnid died in the third highest treatment (2.33 mg/L; TWA) on day one and two animals died on day three in the second highest treatment (5.27 mg/L; TWA)
- Type and number of morphological abnormalities: none
- Type and number of behavioural abnormalities: none - Validity criteria fulfilled:
- yes
- Remarks:
- Please see Table 1 at "Any other information on results incl. tables"
Reference
Table 1: Validity criteria for OECD 211.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The mortality of the parent animals in the control (female Daphnia) does not exceed 20% at the end of the test. |
0% |
yes |
The mean number of living offspring produced per parent animalsurvivingat the end of the test is ≥ 60 in the control. |
65.4 |
yes |
Table 2: Survial, growth and reproduction data.
Concentration |
Parental survival |
Growth |
Age at first brood |
Cumulative offspring per female |
(mg/L) |
(%) |
Mean±SD (mm) |
Mean±SD (days) |
Mean±SD (Ind.) |
Control |
100 |
5.2±0.58 |
10.8±0.5 |
70 ± 10 |
0.51 (nominal) |
100 |
5.2±0.17 |
10.9±0.5 |
65 ± 7 |
1.13 (nominal) |
100 |
5.1±0.39 |
10.8±0.5 |
65 ± 9 |
2.48 (nominal) |
90 |
4.8±0.35 |
10.9±0.5 |
63 ± 12 |
5.45 (nominal) |
80 |
4.7±0.47 |
11.1±1.06 |
60 ± 11 |
12 (nominal) |
100 |
4.8±0.33 |
10.8±0.7 |
58 ± 16 |
Description of key information
NOEC (21 d) : 5.3 mg a.i./L (measured, TWA, OECD 211)
Key value for chemical safety assessment
Additional information
The chronic toxicity of the substance to aquatic invertebrates was tested in a GLP guideline study conducted according to OECD 211. Cu-free tap water according to OECD guideline 211 sourced from the Schmallenberg district water production plants was used as test medium. Juvenile Daphnia magna were exposed the test substance in a semi-static test system for 21 days. The nominal test substance concentrations were 0.51, 1.13, 2.48, 5.45, and 12 mg a.i./L. The substance concentrations in freshly prepared and aged test solution were analytically verified by liquid chromatography (LC) and tandem mass spectrometry detection (LC-MS/MS). The time weighted average (TWA) concentrations were 0.45, 1.03, 2.33, 5.27, and 11.75 mg a.i./L. The effects on growth and survival as well as reproductive performance were investigated in the test. The substance did not affect survival, growth or time of first brood of adult Daphnia. A concentrations related effect on the reproduction rate was observed. The determined NOEC was 5.3 mg a.i./L (measured, TWA) based on reproduction.
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