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EC number: 257-288-8 | CAS number: 51566-62-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
In vitro:
In the chosen key study for mutagenicity in bacteria, i.e. an Ames test, 5 Salmonella typhimurium strains (TA 1535, TA 1537, TA 1538, TA98, and TA 100) were used to test the mutagenic potential of 3,7-dimethyloct-6-enenitrile (0, 0.0007, 0.002, 0.007, 0.02, and 0.06 mg test liquid per 0.1 mL methanol per plate), both with and without metabolic activation (TNO 1980). 3,7-dimethyloct-6-enenitrile did not show any mutagenic activity in any strain up to the bacterial toxic concentrations under the chosen testing conditions.
In the chosen key study for mutagenicity in mammalian cells, i.e. a GLP-compliant gene (HPRT) mutation assay according to OECD guideline 476, chinese hamster lung fibroblast (V79) were exposed to 3,7-dimethyloct-6-enenitrile with and without metabolic activation (Harlan, 2012). No relevant and reproducible increase in mutant colony cells was observed up to the cytotoxic concentration. Therefore, 3,7-dimethyloct-6-enenitrile, is considered to be non-mutagenic in this HPRT assay.
In the chosen key study for cytogenicity in mammalian cells, i.e. a GLP-compliant chromosome aberration test according to OECD guideline 473, chinese hamster lung fibroblast (V79) were exposed to 3,7-dimethyloct-6-enenitrile with and without metabolic activation (RCC, 2008). In the presence of S9 mix, a statistically significant and biologically relevant increase in the number of aberrant cells, excluding gaps was observed after 4 hrs treatment with 500 µg/mL (14.0% aberrant cells, excluding gaps). In addition, in the presence of S9 mix after 4 hrs treatment with 500 µg/mL the rate of polyploid cells (5.7 %) as well as endomitotic cells (1.5 %) and after the 4 hrs treatment with 125 µg/mL the rate of endomitotic cells (0.4 %) were statistically significant increased. Therefore, 3,7-dimethyloct-6-enenitrile is considered to be clastogenic in this chromosome aberration test in the presence of S9 mix. In addition, 3,7-dimethyloct-6-enenitrile, is considered to inhibit mitotic processes and to induce numerical chromosome aberrations in this chromosome aberration test.
In vivo:
In the chosen key study for cytogenicity in vivo, i.e. a GLP-compliant erythrocyte micronucleus test according to OECD guideline 474, 5 NMRI mice per sex per dose were treated once by oral gavage with 3,7-dimethyloct-6-enenitrile (500, 1000, 2000 mg/kg bw) dissolved in olive oil followed by a 24 hour (500, 1000, 2000 mg/kg bw) and 48 hour (2000 mg/kg bw) post-exposure period before sacrificing the animals (BASF, 2004). 3,7-dimethyloct-6-enenitrile did not result in any increase in the rate of micronuclei. The number of normochromatic or polychromatic erythrocytes containing small micronuclei (d < D/4) or large micronuclei (d ≥ D/4) did not deviate from the vehicle control value at any of the sacrifice intervals and was within the historical control range. A slight inhibition of erythropoiesis induced by the treatment of mice with 3,7-dimethyloct-6-enenitrile was detected from about 500 mg/kg body weight onward. Overall, 3,7-dimethyloct-6-enenitrile does not have any chromosome-damaging (clastogenic) effect, and there were no indications of any impairment of chromosome distribution in the course of mitosis (aneugenic activity) in bone marrow cells in vivo.
Short description of key information:
Ames test: negative (TNO 1980)
HPRT test (OECD 476, GLP): negative (Harlan 2012)
Chromosome aberration (OECD 473, GLP): positive (RCC 2008)
Micronucleus test in vivo (OECD 474, GLP): negative (BASF 2004)
Endpoint Conclusion:
Justification for classification or non-classification
Based on the available genotoxicity tests, 3,7-dimethyloct-6-enenitrile does not need to be classified for genotoxicity according to the Directive 67/548/EEC and according to the EU Classification, Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC) No. 1272/2008.
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