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EC number: 226-733-8 | CAS number: 5459-93-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 9.5. – 8.6. 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Cyclohexyl(ethyl)amine
- EC Number:
- 226-733-8
- EC Name:
- Cyclohexyl(ethyl)amine
- Cas Number:
- 5459-93-8
- Molecular formula:
- C8H17N
- IUPAC Name:
- N-ethylcyclohexanamine
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): N-ethylcyclohexylamine
- Physical state: clear colourless liquid
- Analytical purity: 99.5% (w/w)
- Impurities (identity and concentrations): cyclohexanol 0.22% (w/w), alifatic amines 0.23% (w/w), water 0.07% (w/w)
- Lot/batch No.: 89002001
- Expiration date of the lot/batch: 12/2012
- Storage condition of test material: in original packaging at the fridge
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic, non-adapted
- Details on inoculum:
- INOCULUM
A mixed population of microorganisms obtained from the secondary effluent of the wastewater treatment plant of Pardubice processing predominantly municipal sewage.
PREPARATION OF THE INOCULUM
The fresh collected wastewater was filtered through paper filter. COD was determined and wastewater was aerated till using.
JUSTIFICATION OF THE TEST SYSTEM
The inoculum preparation is in conformity with the recommendations of the test guidelines. - Duration of test (contact time):
- 0 - 28 d
Initial test substance concentration
- Initial conc.:
- 3.38 - 3.39 mg/L
- Based on:
- COD
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Mineral medium: preparation of mineral medium in accordance to Method C.4E - Closed Bottle Test, Council Regulation (EC) No. 440/2008, published in O. J. L 142, 2008.
- Inoculated medium: The mineral medium was strongly aerated for 20 min. and let standing for 20 h at the test temperature. The volume of wastewater for the inoculation of mineral medium was chosen 2.0 mL per 1 L of medium. 30 L aerated mineral medium was prepared for the test and before the beginning of the test it was inoculated with 60 mL of modified wastewater. The pH value of medium: 7.4.
- Test temperature: 20+/-1°C
- Illumination: in the dark
- pH adjusted: no
TEST SYSTEM
- Series of bottles for the test:
- 2 x 9 bottles with test substance and inoculated medium
- 2 x 9 bottles with reference substance and inoculated medium for the check of inoculum activity
- 2 x 5 bottles with test and reference substance mixture and inoculated medium for the toxicity test
- 2 x 9 bottles with inoculated medium only for the blank determination
TEST SYSTEM
- Measuring equipment: equipment for the determination of dissolved oxygen
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, the inoculated mineral medium
- Toxicity control: yes, the test and reference substance mixture
MEASUREMENT:
- analysis for dissolved oxygen by the Winkler method at the zero-time, 3rd, 7th, 10th, 14th, 17th, 21st, 24th and 28th day.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0.6
- Sampling time:
- 28 d
BOD5 / COD results
BOD5 / CODopen allclose all
- Parameter:
- COD
- Value:
- 200 other: mg.L-1
- Parameter:
- BOD5
- Value:
- 9 other: mg.L-1
- Results with reference substance:
- COD of reference substance: 1.642 mg.mg-1, biodegradation 87% after 28 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- In this 28-day study of ready biodegradability a degradation of 0.6 % of the test substance, N-ethylcyclohexylamine, was attained in the end of study.
- Executive summary:
The test substance, N-ethylcyclohexylamine, was tested for the ready biological degradability in Closed Bottle Test.
Test performance
The test was performed according to: Method C.4E - Closed Bottle Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
The results of biological degradation are related to experimentally determined COD values
of test and reference substance at the beginning of the test.
Owing to the limited water solubility of test substance dosing the dispersion in mineral medium into each bottle was implemented.
Sodium benzoate was used as the reference substance. The dosage was carried out from the stock solution.
COD of the test substance in medium at the beginning of the main test: 3.38 - 3.39 mg.L-1
COD of reference substance in medium at the beginning of the main test: 3.45 mg.L-1
In parallel to the main test the toxicity test was performed.
Based on the test substance contains nitrogen in the chemical composition, the oxidized nitrogen forms were determined and the correction for nitrification was carried out.
The test was performed at temperature of 20 ± 1 °C with the pH values of solutions 7-8 at the beginning of the test.
Validity of test
The prescribed validity criteria in the test were fulfilled.
The test substance was not inhibiting for the used inoculum.
Since all criteria of acceptability were met, this study is considered to be valid.
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