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EC number: 201-944-8 | CAS number: 89-83-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from publication.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- I. Acute oral toxicity.
- Author:
- Jenner et al.
- Year:
- 1 964
- Bibliographic source:
- Food and Cosmetics Toxicology
- Reference Type:
- publication
- Title:
- Final Report on the Safety Assessment of test chemical
- Author:
- COSMETIC INGREDIENT REVIEW
- Year:
- 2 006
- Bibliographic source:
- International Journal of Toxicology
- Reference Type:
- review article or handbook
- Title:
- Acute oral toxicity of test chemical
- Author:
- Richard J. Lewis
- Year:
- 2 012
- Bibliographic source:
- Sax's Dangerous Properties of Industrial Materials
- Reference Type:
- review article or handbook
- Title:
- Acute oral toxicity of test substance
- Author:
- S D Gangolli
- Year:
- 2 007
- Bibliographic source:
- The Dictionary of Substances and their Effects (DOSE): T-Z and Index
- Reference Type:
- review article or handbook
- Title:
- oral toxicity of test chemical
- Author:
- Michael Ash
- Year:
- 2 004
- Bibliographic source:
- Handbook of Green Chemicals
- Reference Type:
- review article or handbook
- Title:
- Acute oral toxicity - Test chemical
- Author:
- Kirk-Othmer
- Year:
- 2 012
- Bibliographic source:
- Kirk-Othmer Chemical Technology of Cosmetics
- Reference Type:
- review article or handbook
- Title:
- Acute oral toxicity in rat by using test chemical
- Author:
- Victor O. Sheftel
- Year:
- 2 000
- Bibliographic source:
- Indirect Food Additives and Polymers
- Reference Type:
- review article or handbook
- Title:
- Acute oral toxicity:Test chemical
- Author:
- Maryadele J ‘O Neil
- Year:
- 2 006
- Bibliographic source:
- Merck Index - An Encyclopedia of Chemicals, Drugs, and Biologicals
- Reference Type:
- other: authoritative database
- Title:
- Acute oral toxicity in rat.
- Author:
- U.S. National Library of Medicine
- Year:
- 2 017
- Bibliographic source:
- ChemIDplus
- Reference Type:
- other: authoritative database
- Title:
- Acute toxicity - test chemical
- Author:
- IFA GESTIS
- Year:
- 2 018
- Bibliographic source:
- GESTIS database
- Reference Type:
- other: authoritative database
- Title:
- Acute toxicity : Test Chemical
- Author:
- U.S. National Library of Medicine
- Year:
- 2 015
- Bibliographic source:
- HSDB
- Reference Type:
- review article or handbook
- Title:
- Acute Oral Toxicity : Test substance
- Author:
- Council of Europe
- Year:
- 2 006
- Bibliographic source:
- Plants in cosmetics
- Reference Type:
- secondary source
- Title:
- Acute oral toxicity of test chemical conducted in rat
- Author:
- RTECS
- Year:
- 2 017
- Bibliographic source:
- RTECS
- Reference Type:
- secondary source
- Title:
- Test substance used to determine acute toxicity
- Author:
- U.S. National Library of Medicine
- Year:
- 2 017
- Bibliographic source:
- TOXNET
- Reference Type:
- secondary source
- Title:
- BIOPESTICIDE REGISTRATION ACTION DOCUMENT
- Author:
- U.S. Environmental Protection Agency
- Year:
- 2 006
- Bibliographic source:
- U.S. Environmental Protection Agency
- Reference Type:
- secondary source
- Title:
- Conclusion on the peer review of the pesticide risk assessment of the active substance
- Author:
- EFSA
- Year:
- 2 012
- Bibliographic source:
- EFSA Journal
- Reference Type:
- secondary source
- Title:
- Test chemical: Reregistration Eligibility Decision (RED)
- Author:
- U. S. Environmental Protection Agency (EPA)
- Year:
- 1 993
- Bibliographic source:
- U. S. Environmental Protection Agency (EPA)
- Reference Type:
- secondary source
- Title:
- DAR - test chemical
- Author:
- European Food Safety Agency (EFSA)
- Year:
- 2 011
- Bibliographic source:
- European Food Safety Agency
- Reference Type:
- secondary source
- Title:
- Phenols in Flavor Usage
- Author:
- Food and Drug Administration
- Year:
- 1 985
- Bibliographic source:
- National Technical Information Service
- Reference Type:
- secondary source
- Title:
- Up to date review of toxicological data of some plant volatiles with antifungal activity
- Author:
- Food and Drug Administration
- Year:
- 1 999
- Bibliographic source:
- National Technical Information Service
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- Acute oral toxicity of Thymol (CAS no: 89-83-8) in rat.
- GLP compliance:
- not specified
- Test type:
- other: not specified
- Limit test:
- no
Test material
- Reference substance name:
- Thymol
- EC Number:
- 201-944-8
- EC Name:
- Thymol
- Cas Number:
- 89-83-8
- Molecular formula:
- C10H14O
- IUPAC Name:
- 5-methyl-2-(propan-2-yl)phenol
- Details on test material:
- - IUPAC Name: Thymol- InChI: 1S/C10H14O/c1-7(2)9-5-4-8(3)6-10(9)11/h4-7,11H,1-3H3- Smiles: c1(c(cc(C)cc1)O)C(C)C- Molecular formula :C10H14O- Molecular weight :150.2196 g/mol- Substance type:Organic- Physical state:Solid, white crystalline powder, thyme-like, aromatic odor
Constituent 1
- Specific details on test material used for the study:
- - IUPAC Name: Thymol- InChI: 1S/C10H14O/c1-7(2)9-5-4-8(3)6-10(9)11/h4-7,11H,1-3H3- Smiles: c1(c(cc(C)cc1)O)C(C)C- Molecular formula :C10H14O- Molecular weight :150.2196 g/mol- Substance type:Organic- Physical state:Solid, white crystalline powder, thyme-like, aromatic odor
Test animals
- Species:
- rat
- Strain:
- Osborne-Mendel
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS- Fasting period before study: fasted for approximately 18 hr prior to treatment
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- propylene glycol
- Details on oral exposure:
- VEHICLE- Concentration in vehicle: 20%
- Doses:
- 980 (817-1180)mg/kg bw
- No. of animals per sex per dose:
- groups of 5/sex
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days - Frequency of observations and weighing: Animals were observed for one or two weeks for clinical signs and body weight effects.
- Statistics:
- LD50’s were computed by the method of Litchfield & Wilcoxon (1949).
Results and discussion
- Preliminary study:
- not specified
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 980 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 817 - 1 180
- Remarks on result:
- other: 50% mortality observed
- Mortality:
- Rats died within 4 h to 5 days after dosing
- Clinical signs:
- other: Depression and ataxia were noted in most dose groups and coma at greater doses.
- Gross pathology:
- not specified
- Other findings:
- not specified
Any other information on results incl. tables
Table - Acute oral toxicity of food flavourings and compounds of related structure:
Compound | Concentration (w/v) and solvent used | Species | LD50 with 95 % confidence limits (mg/kg | Slope function with 95% confidence limits | Toxic signs and death time (D.T.) |
Thymol (p-cymen-3-ol,3- Hydroxyl-p-cymene) | C - 20% | Rat | 980 (817-1180) | 1.6 (1.3-2.0) | Depression, ataxia, coma on high doses D.T. 4 hr-5 days |
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The acute oral LD50 value was considered to be 980 mg/kg (with 95% confidence limits of 817 to 1180 mg/kg), when groups of 5 male and female Osborne-Mendel rats were treated with Thymol (CAS no: 89-83-8) via oral gavage route.
- Executive summary:
Acute oral toxicity study of Thymol (CAS no: 89-83-8) was conducted in groups of 5 male and female Osborne-Mendel rats at the dose concentration of 980 (817-1180) mg/kg bw. The given test chemical dissolved at 20% in propylene glycol and administered via oral gavage route. Animals were observed for one or two weeks for clinical signs and body weight effects. LD50’s were computed by the method of Litchfield & Wilcoxon (1949). Rats died within 4 h to 5 days after dosing. Depression and ataxia were noted in most dose groups and coma at greater doses. Therefore, LD50 was considered to be 980 mg/kg (with 95% confidence limits of 817 to 1180 mg/kg), when groups of 5 male and female Osborne-Mendel rats were treated with Thymol via oral gavage route.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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