[1,1'-Biphenyl]-2-carbonitrile, 4'-[(2-butyl-4-oxo-1,3-diazaspiro[4.4]non-1-en-3-yl)methyl]-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 April 1996 to 3 July 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline Study; GLP compliance

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4'-((2-butyl-4-oxo-1,3-diazaspiro[4.4]non-1-ene-3-yl)methyl)(1,1'-biphenyl)-2-carbonitrile

EC Number:

604-075-6

Cas Number:

138401-24-8

Molecular formula:

C25H27N3O

IUPAC Name:

4'-((2-butyl-4-oxo-1,3-diazaspiro[4.4]non-1-ene-3-yl)methyl)(1,1'-biphenyl)-2-carbonitrile

Details on test material:

- Name of test material (as cited in study report): SR47563
- Chemical name: Spiromethyl biphenyl nitrile
- Physical state: white-beige powder
- Lot/batch No.: 6R00003

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): station d'epuration, de Chantilly - 60
- Initial cell/biomass concentration: 1*10^7 microoganisms/ml

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

40.4 other: mg/3L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

81.5 other: mg/3L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

Study design according to the guideline for the Modified Sturm Test with the following test vessels:
1. Inoculum control (FB Inoc.);
2. Test substance low (FE1 10 mg/l);
3. Test substance high (FE1 20 mg/l);
4. Positive control (FC Anil.);
5. Inhibition control (FI(Inh));
6. Abiological control (FA(abiot)).

Reference substance

Reference substance:

aniline

Results and discussion

Preliminary study:

Not performed.

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

10 d

Remarks on result:

other: For both concentrations

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

14 d

Remarks on result:

other: For both concentrations

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

21 d

Remarks on result:

other: For both concentrations

% Degradation 4

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

28 d

Remarks on result:

other: For both concentrations

BOD5 / COD results

Results with reference substance:

Positive control: More than 70 % degradation within 14 days, validity criterion was met.
Inhibition control: More than 25 % degradaton of the mixture of aniline with the test substance, validity criterion was met.
Also the validity criteria of the inoculum and abiotic degradation were met.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test substance shows not to be degradable under te conditions of a test for readily biodegradability (Modified Sturm Test).

Executive summary:

In a modified Sturm Test, Spiromethyl biphenyl nitrile was exposed to micro-organisms in activated sludge from a domestic waste water plant. Concentrations corresponding with organic carbon contents of 10 and 20 mg/l were tested together with aniline as positive control. No detectable degradation of Spiromethyl biphenyl nitrile was recorded at either levels, while all validity criteria were met.