Registration Dossier
Registration Dossier
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EC number: 609-664-1 | CAS number: 393-85-1
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EC Directive 92/69/EC Method C.3, Official Journal of the European Communities No. L383A/179
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 5-Fluoro-2-nitrobenzoic acid methyl ester
- EC Number:
- 609-664-1
- Cas Number:
- 393-85-1
- Molecular formula:
- C8H6FNO4
- IUPAC Name:
- 5-Fluoro-2-nitrobenzoic acid methyl ester
- Test material form:
- other: yellowish liquid
- Details on test material:
- Purity: 96.6 %
Constituent 1
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Study design
- Test type:
- static
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 23+/- 2 °C
- Nominal and measured concentrations:
- Nominal concentration: 3.13- 6.25 -12.5-25-50-100 mg/L
- Details on test conditions:
- Light regime: 24 h/day, Temperature: 23+/- 2 °C, replicates: 3 per test condition and 6 control replicates, test volume: 100 ml
Results and discussion
- Reported statistics and error estimates:
- The BONFERRONI t-test was carried out for the determination of statistically significant differences compared to control replicates. When running a BONFERRONI t-test a Normaility test and Homogeneity test were done first. P-value for both were 0.05. The alpha value for the BONFERRONI t-test was 0.05.
Applicant's summary and conclusion
- Conclusions:
- In this study 5-Fluoro-2-nitrobenzoic acid methyl ester was found to inhibit the growth of the freshwater green alga Desmodesmus Subspicatus at nominal concentrations > 12 .5 mg/L (biomass and growth rate). The EC50 values with 95 % confidence intervals for inhibition of biomass growth (Eb50) and specific growth rate (Er50) after 72 h were 12.6 (12.2 -13.1) and 14.5 (14.1 -14.9) mg/l.
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