Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: study was carried out according to test guidline and under GLP-conditions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report Date:
1997

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
static
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
2.1 - 2.5 mmol Ca2+ + Mg2+/L
Test temperature:
21.7 - 22.0°C
pH:
7.7 -7.9
Dissolved oxygen:
7.3 - 8.8 mg/L
Nominal and measured concentrations:
0/10000 mg/L (nominal)
0/0.33 mg/L (mean of measured conc.)
Details on test conditions:
use of reconstituted water, composition acc. to ISO/DIS 7346/1
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: lethality value based on loading rates (nominal conc.); mean value of measured concentrations: 0.33 mg/L
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: lethality value based on loading rates (nominal conc.); mean value of measured concentrations: 0.33 mg/L
Duration:
96 h
Dose descriptor:
LC100
Remarks on result:
other: not determined

Applicant's summary and conclusion

Conclusions:
67/548/EEC: Acute aquatic toxicity: no classification
1272/2008/EC: Acute aquatic toxicity: no classification
Executive summary:

No lethality was observed up to the solubility limit of Naphtol AS-ITR. The 10000 mg/L (0.33 mg/L*) group exhibited reversible symptoms.

* = value based on the mean of analytically determined values