Effects of α2u-globulin accumulating agents on α2u-globulins in rat kidneys were examined by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting analysis. Treatment of male animals with decalin (150 mg/kg), 2,2,4-trimethylpentane (50 mg/kg), isophorone (150 mg/kg),d-limonene (150 mg/kg) or 1,4-dichlorobenzene (150 mg/kg) by gavage for 14 consecutive days in each case resulted in a marked intensification of a protein band corresonding to kidney-type-α2u-globulin with a molecular mass calculated to be α2u-globulin band, caused the intensification of a α2u-globulin by SDS-PAGE and immunoblotting. These results indicated that at least two types of α2u-globulin can be increased in male rat kidney by chemical treatment. Moreover, cystein protease(s) appear(s) to play an important role in the degradation of α2u-globulin and particularly in the conversion of native-type-α2u-globulin to kidney-type-α2u-globulin in rat kidneys.
Registration Dossier
Registration Dossier
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EC number: 202-046-9 | CAS number: 91-17-8
Specific investigations: other studies
Administrative data
- Endpoint:
- biochemical or cellular interactions
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Differences in a2u-globulins increased in male rat kidneys following treatment with several a2u-globulin accumulating agents: cystein protease(s) play(s) an important role in production of kidney-type-a2u-globulin
- Author:
- Saito K, Kaneko H, Isobe N, Nakatsuka I, Yoshitake A and Yamada H
- Year:
- 1 992
- Bibliographic source:
- Toxicology 76, 177-186
Materials and methods
Test material
- Reference substance name:
- Decahydronaphthalene
- EC Number:
- 202-046-9
- EC Name:
- Decahydronaphthalene
- Cas Number:
- 91-17-8
- Molecular formula:
- C10H18
- IUPAC Name:
- decahydronaphthalene
- Reference substance name:
- Decalin
- IUPAC Name:
- Decalin
- Details on test material:
- trans-decahydronaphthalene from Wako Pure Chemical Industries Ltd, purity > 97 %
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- not specified
- Sex:
- male
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- not specified
- Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 14 days
- Frequency of treatment:
- daily
Doses / concentrations
- Remarks:
- Doses / Concentrations:
150 mg/kg bw/d
Basis:
no data
Results and discussion
Applicant's summary and conclusion
- Conclusions:
- The results of this study indicated that at least two types of α2u-globulin can be increased in male rat kidney by chemical treatment.
- Executive summary:
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