Manganese, [[2,2',2''-[nitrilotris[2,1-ethanediyl(nitrilo-.kappa.N)methylidyne]]tris[phenolato-.kappa.O]](3-)]-, (OC-6-22)-

Hydrolysis

Administrative data

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002-10-2 to 2003-01-28

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD guideline study, GLP. No analytical method could be established to evaluate the hydrolytic behaviour of the test item. From preliminary experiments a fast hydrolytic degradation could be observed.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 111 (Hydrolysis as a Function of pH)

Version / remarks:

adopted May 12, 1981

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

479-540-8

EC Name:

-

Cas Number:

61007-89-4

Molecular formula:

C27 H27 Mn N4 O3

IUPAC Name:

manganese(3+) 2-[({2-[bis(2-{[(2-oxidophenyl)methylidene]amino}ethyl)amino]ethyl}imino)methyl]benzen-1-olate

Radiolabelling:

no

Study design

Details on test conditions:

Preliminary experiments:
- 0.267 mg of the test item were weighed into a 25mL flask that was filled up to the mark with N,N-dimethylformamide. After vigorous shaking the test item was completely dissolved and a UV/Vis spectra could be recorded.
- 2 mL ofthe dimethylformamide solution were transeferred into the cuvette, a spectra was recorded, and after addition of 0.5 mL of 0.1 N hydrochloric acid solution 14 spectra were recorded within 9 minutes.
- This procedure was also done with:
- buffer solution pH 4.0 (11 spectra within 35 minutes)
- buffer solution pH 7.0 (9 spectra within 13 minutes)
- buffer solution pH 9.0 (11 spectra within 21 minutes)
To compare the spectra ofthe test item a spectra of a 1.19 mg/100 mL solution of salicylaldehyde in water was recorded, and also a spectra of a 0.354mg/25mL solution in DMF of the ligand of the test item.

To show the very poor solubility of the test item the following solutions were prepared, sonificated for 5 minutes, filtered through a 0.22 µ disposable filter unit and a spectra was recorded from the filtrate:
- 0.399 mg in 25mL buffer solution pH 9.0
- 0.387 mg in 25mL buffer solution pH 9.0 with 1% dimethylformamide as cosolvent
- 0.448 mg in 25mL buffer solution pH 7.0
- 0.409 mg in 25mL double distilled water
All experiments have been done at room temperature, 22 - 23 °C.

Results and discussion

Preliminary study:

The performed preliminary measurements yield to the following result:
The test substance showed extremely hydrolytic behaviour and has a very poor solubility in the higher pH range.
• In the pH range of 1.0 to 2.0 a nearly complete degradation of the test item takes place within < 10 minutes at room temperature.
• in the pH range 4.0 to 6.9 also a very fast degradation could be observed.
• in the pH range 7.0 to 13.5 the solubility of the test item was too poor even in the presence of Dimethylformamide as cosolvent for studying the iiydrolytic behaviour.
• In none of the aqueous solutions the typical absorbance for a Mn(lll) complex at the wavelength of about 600nm could be observed, which is a clear indication for the hydrolytic behaviour.

Any other information on results incl. tables

No analytical method could be established to evaluate the hydrolytic behaviour of the test item. From preliminary experiments a fast hydrolytic degradation could be observed in the pH range 1 to 7, at pH > 7 the solubility of the test item is too poor for further experiments.