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EC number: 801-093-8 | CAS number: 1315251-11-6
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
Description of key information
An OECD TG 222 study in compliance with GLP criteria with Earthworm (Eisenia andrei) is available for the substance. Groups of 10 earthworms per replicate (40 per concentration for test item treatments and 80 per control) were exposed to nominal concentrations of 1.00, 3.00, 9.00, 27.0, 90.0, 270, and 520 mg/kg soil dw for 56 days in a laboratory study with artificial soil. The test condition was maintained at 18 - 22 °C, 51.7 – 56.8% maximum water holding capacity and 16 hours light and 8 hours darkness light cycle (400 lux to 800 lux light intensity). The adult earthworm mortality, behavioural effects and biomass development was studied after 28 days exposure. Moreover, the reproduction rate (number of offspring) was assessed after additional 28 days (assessed 56 days after application).
A mortality of 12.5% and 97.5% was found at the test concentrations of 270 and 520 mg test item/kg soil dw, respectively, which was statistically significantly different compared to the control, where 0% of the earthworms died. The body weight changes of the earthworms after 4 weeks exposure to the substance were not statistically significantly different compared to the control up to and including the test concentration of 90.0 mg test item/kg soil dw. At the test concentration of 270 mg test item/kg soil dw and above the body weight changes of the earthworms were statistically significantly reduced compared to the control. The reproduction rates were not statistically significantly different compared to the control up to and including the test concentration of 9.00 mg test item/kg soil dw. At the test concentration of 27.0 mg test item/kg soil dw and above, reproduction was statistically significantly reduced compared to the control. No behavioural abnormalities were observed in any of the treatment groups. The feeding activity in all the treated groups was comparable to the control up to and including the test concentration of 90.0 mg test item/kg soil dw. At the test concentration of 270 mg test item/kg soil dw and above, the feeding activity was reduced. Based on these findings, the 56-d EC10 for reproduction was determined to be 14.3 mg/kg soil dw.
Key value for chemical safety assessment
- Long-term EC10, LC10 or NOEC for soil macroorganisms:
- 14.3 mg/kg soil dw
Additional information
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