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EC number: 218-076-0 | CAS number: 2049-95-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 6.25, 12.5, 25, 50 and 100 % of a saturated stock solution.
- Sampling method: At test initiation bulk solutions were sampled. After 24, 48 and 72 hours samples were taken from the test flasks.
- Sample storage conditions before analysis: 2-8°C. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: An amount of 200 mg tertiary-amylbenzene was dissolved in 2 litres algal medium. This stock solution was continuously stirred and placed at a temperature of 20°C. On the day of test initiation the stock solution was filtered with filter paper (S&S 595 1/2) to remove undissolved
test substance.
- Controls: controls with test medium only were included.
- Evidence of undissolved material: No. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Selenastrum capricornutum
- Strain: ATCC 22662
- Source: Culture Collection of Algae and Protozoa, Ambleside, Cumbria, UK
- Age of inoculum (at test initiation): 4 days.
- Method of cultivation: Each week an inoculum (1 ml) is transferred into fresh algal medium. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Not applicable.
- Hardness:
- No measurement.
- Test temperature:
- 23.0 - 23.8°C
- pH:
- Controls: 8.1 (day 0) and 9.5 (day 3 )
Test concentrations: 8.5 - 9.5 (day 3) - Dissolved oxygen:
- No measurement
- Nominal and measured concentrations:
- See Table 1.
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: closed
- Material, size, headspace, fill volume: closed (glass) flasks with a volume of 100 ml were completely filled with test solution
- Aeration: No
- Initial cells density: 1.0 x 10E4 cells/ml
- Control end cells density: 75 x 10E4 cells/ml
- No. of vessels per concentration (replicates): 6 replicates
- No. of vessels per control (replicates): 6 replicates
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: not applicable, the medium was prepared in purified water
- Culture medium different from test medium: No.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 24 hours light
- Light intensity and quality: 76 µE.m-2.s-1 which is in agreement with the validity criterion of 60 – 120 µE.m-2.s-1.
- Other: During the test period, the flasks were placed in a horizontal position in a shaking incubator in a random order.
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: the absorption of the test solutions at 750 nm was measured after 24, 48 and 72 hours using a spectrophotometer. After 24 hours, 1 replicate vessel was used for measurement; after 48 hours, 2 replicate vessels were used and after 72 hours, 3 replicates were used.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 (0, 6.25, 12.5, 25, 50 and 100 % of a saturated stock solution). - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.9 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.8 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Details on results:
- See Table 2.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions of this study, The EC50/72h for biomass integral and growth rate were 3.2 and 3.9 mg/L respectively. The NOEC, based on mean measured concentrations was 0.80 mg/L.
- Executive summary:
The acute toxicity of Tertiary-amylbenzne to algae (Selenastrum capricornutum) was tested according to OECD Guidelien 201 and in accordance with GLP compliance.
Based on a preliminary test the study was conducted at 0, 6.25, 12.5, 25, 50 and 100% of the filtrated stock solution. Because the test substance will volatilise from the test solutions, the test was conducted in closed flasks with a volume of 100 mL. The biological endpoints based on the average of the measured concentrations being 0, 0.53, 0.80, 1.4, 2.6 and 5.7 mg/L.
The cell density increased 75 times in the controls, which meets the validity criterion of a minimal 16 times increase. The pH of controls was 8.1 on day 0 and 9.5 on day 3 which is in accordance with the validity criterion of a maximum increase of 1.5 pH unit.
Under the test conditions of this study, the EC50/72 hours for biomass integral and growth rate were 3.2 and 3.9 mg/L respectively; The NOEC, based on biomass was 0.80 mg/L.
Reference
Table 2 Mean measured algal cell densities during the test period
Mean measured concentration (mg/l) |
Cell density x 104 (cells/ml) |
||
Day 1 (n=1) |
Day 2 (n=2) |
Day 3 (n=3) |
|
controls 0.53 0.80 1.4 2.6 5.7 |
4.653 4.706 4.466 3.418 3.353 1.462 |
16.91 15.91 17.40 17.56 13.55 1.976 |
75.35 79.51 74.03 64.99 48.67 1.698 |
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 3.9 mg/L
- EC10 or NOEC for freshwater algae:
- 0.8 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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