Registration Dossier
Registration Dossier
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EC number: 211-711-2 | CAS number: 689-67-8
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 6,10-dimethylundeca-5,9-dien-2-one
- EC Number:
- 211-711-2
- EC Name:
- 6,10-dimethylundeca-5,9-dien-2-one
- Cas Number:
- 689-67-8
- Molecular formula:
- C13H22O
- IUPAC Name:
- 6,10-dimethylundeca-5,9-dien-2-one
- Details on test material:
- - Name of test material (as cited in study report): Geranylacetone
- Analytical purity: 98.1 % (sum of the two isomers)
- Isomers composition: Z-Isomer: 39.1 %
; E-lsomer: 59.0 %
- Lot/batch No.: Kampagne 05/05
Water solubilty: 0.039 mg/l
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal activated sludge from treatment plant of the city Mannheim/Baden Wuerttemberg (Germany). Concentration of the activated sludge suspension = 6.0 g/l of dry weight. It was then added to the test vessels to obtain a sludge concentration of 30 mg/l dry substance
- Preparation of inoculum for exposure: the activated sludge was sieved and pre-aerated for 48 hours before the test starts. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 25 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22 +/- 2°C
- pH adjusted: before pre-incubation the pH-values of the mineral medium were adjusted in all test vessels to 7.4
TEST SYSTEM
- Culturing apparatus: 2 l incubation bottles filled up to volume of 1.5 l
- Weighed sample of test substance (mg/vessel): 37
- Deionised water (ml/vessel): 1473
- Inorganic medium (ml/vessel): 19.5
- Inoculum (ml/vessel): 7.5
- Number of replicates: test substance 2
blank control 2
reference control 1
inhibition control 1
- Sampling frequency: twice a week the TIC values of the adsorption solutions of each trap were determined
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 70 - 80
- Sampling time:
- 28 d
- Details on results:
- Kinetic of test substance (in %):
< 10 after 4 day(s)
= 14 after 5 day(s)
= 63 after 14 day(s)
= 72 after 24 day(s)
= 74 after 28 day(s)
Kinetic of control substance (in %):
< 10 after 3 day(s)
= 72 after 10 day(s)
Applicant's summary and conclusion
- Interpretation of results:
- readily biodegradable
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