Reaction mass of 4-Vinylcyclohexene, Cyclododeca-1,5,9-triene (1Z,5E,9E) and Cycloocta-1,5-diene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

a solubilizer was used in this study; However, as the effect values were still above water solubility, the results can be regarded worst case and no effects up to the water solubility at 0.28 mg/L

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2003

Materials and methods

Principles of method if other than guideline:

Method: no data

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(1Z,5E,9E)-cyclododeca-1,5,9-triene

Molecular formula:

C12H18

IUPAC Name:

(1Z,5E,9E)-cyclododeca-1,5,9-triene

Details on test material:

Test substance: 1,5,9-cyclododecatriene, purity 100 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Remarks:

acetone

Test organisms

Test organisms (species):

Americamysis bahia (previous name: Mysidopsis bahia)

Details on test organisms:

TEST ORGANISMS
- Feeding during test: brine shrimp nauplii twice each day
- Control groups: dilution water; solvent 5.0 ml/l acetone

Study design

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

22 +/- 1 degree C

Dissolved oxygen:

> 60% saturation for first 48 hours, > 40% saturation  thereafter

Nominal and measured concentrations:

- Nominal concentrations: 0.0625; 0.125; 0.25; 0.5; 1.0 mg/l

Details on test conditions:

TEST SYSTEM
- Renewal of test solution: no
- Number of replicates, individuals per replicate: 2 replicates with 10  individuals each

TEST CONDITIONS
- Intensity of irradiation: daylight 40-100 fc - Photoperiod: 16 hours daylight, 8 hours dark

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

0.47 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 0.39 - 0.60 mg/l

Details on results:

- All deaths occurred within 48 hours
- Other effects: Twitching in all organisms at 1 mg/l

Any other information on results incl. tables

Effect data (Immobilisation): 

Test concentration	Immobile animals / Total
control	1/20
solvent control	0/20
0.0625 mg/l	0/20
0.125 mg/l	1/20
0.25 mg/l	0/20
0.50 mg/l	12/20
1.0 mg/l	20/20

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The EC50 (96 hours) was calculated to be 0.47 mg/L indicating that the test substance may be very toxic to aquatic invertebrates. However, the effect value is above the limit of water solubility (0.28 mg/L at 20 °C; AQura, 2010). Therefore, no toxic effect is expected up to the limit of water solubility.

Executive summary:

Daphnia magna were exposed to the test substance over a period of 96 h. Five concentrations ranging from 0.0625 to 1.0 mg/L were tested. The EC50 (96 h) was estimated to be 0.45 mg/L.

The study was assessed as "reliable without restrictions".