Benzoic acid, 4-nitro-, 2-ethylhexyl ester

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

p-Nitrobenzoic acid-2-ethylhexylester

EC Number:

605-350-3

Cas Number:

16397-70-9

Molecular formula:

C15 H21 N O4

IUPAC Name:

p-Nitrobenzoic acid-2-ethylhexylester

Details on test material:

- Name of test material (as cited in study report): P-nitrobenzoesäure2-ethylhexylester
- Substance type:chemical
- Physical state: liquid
- Analytical purity: 99.5 %
- Lot/batch No.: 22 170/85
- Stability under test conditions: given
- water solubility: 1.4 mg/L

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The product was added to the test water separately for each concentration by mixing with 1 g Tween 80 (0.1 g/L test water); subsequently aqua bidest was added in small quantities and then this mixture was transferred into the test water using an ultra-turrax stirrer. Analogously 0.1 g Tween 80 /L test water was added to a carrier control. Subsequently, the fish were placed into the aquaria.
- Controls: water, solvent
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): tween 80
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 100 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): yes, at >= 21.5 mg/L undissolved test substance was visible at the test aquaria

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium GmbH, D-W3422 Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 3.02 cm mean; 2.7 - 3.4 cm range
- Weight at study initiation (mean and range, SD): 0.32 g mean; 0.19 - 0.45 g range
- Feeding during test: no

ACCLIMATION
- Acclimation period: about 3 month
- Acclimation conditions (same as test or not):as test conditions
- Type and amount of food: Tetra Min, ad libitum
- Feeding frequency: daily
- Health during acclimation (any mortality observed): 0.3 % mortality during the last 2 weeks prior test start

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

22-23 °C

pH:

8.2-8.7

Dissolved oxygen:

3.3-8.1

Nominal and measured concentrations:

nominal: 0 (water + solvent), 2.15, 4.64, 10.0, 21.5, 46.4, 100.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: all glass aquaria (30 * 22 * 24 cm)
- Type (delete if not applicable): open
- Material, fill volume: glass, 10 L test volume
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.32 g fish/L water

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 203
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16: 8 h day night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Test concentrations: 0 (water + solvent), 2.15, 4.64, 10.0, 21.5, 46.4, 100 mg/L

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

10.851 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)