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EC number: 605-350-3 | CAS number: 16397-70-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- p-Nitrobenzoic acid-2-ethylhexylester
- EC Number:
- 605-350-3
- Cas Number:
- 16397-70-9
- Molecular formula:
- C15 H21 N O4
- IUPAC Name:
- p-Nitrobenzoic acid-2-ethylhexylester
- Details on test material:
- - Name of test material (as cited in study report): P-nitrobenzoesäure2-ethylhexylester
- Substance type:chemical
- Physical state: liquid
- Analytical purity: 99.5 %
- Lot/batch No.: 22 170/85
- Stability under test conditions: given
- water solubility: 1.4 mg/L
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The product was added to the test water separately for each concentration by mixing with 1 g Tween 80 (0.1 g/L test water); subsequently aqua bidest was added in small quantities and then this mixture was transferred into the test water using an ultra-turrax stirrer. Analogously 0.1 g Tween 80 /L test water was added to a carrier control. Subsequently, the fish were placed into the aquaria.
- Controls: water, solvent
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): tween 80
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 100 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): yes, at >= 21.5 mg/L undissolved test substance was visible at the test aquaria
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium GmbH, D-W3422 Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 3.02 cm mean; 2.7 - 3.4 cm range
- Weight at study initiation (mean and range, SD): 0.32 g mean; 0.19 - 0.45 g range
- Feeding during test: no
ACCLIMATION
- Acclimation period: about 3 month
- Acclimation conditions (same as test or not):as test conditions
- Type and amount of food: Tetra Min, ad libitum
- Feeding frequency: daily
- Health during acclimation (any mortality observed): 0.3 % mortality during the last 2 weeks prior test start
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 22-23 °C
- pH:
- 8.2-8.7
- Dissolved oxygen:
- 3.3-8.1
- Nominal and measured concentrations:
- nominal: 0 (water + solvent), 2.15, 4.64, 10.0, 21.5, 46.4, 100.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: all glass aquaria (30 * 22 * 24 cm)
- Type (delete if not applicable): open
- Material, fill volume: glass, 10 L test volume
- Aeration: yes
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.32 g fish/L water
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 203
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16: 8 h day night regime
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and symptoms of intoxication after 1, 4, 24, 48, 72 and 96 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Test concentrations: 0 (water + solvent), 2.15, 4.64, 10.0, 21.5, 46.4, 100 mg/L
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 10.851 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.