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Diss Factsheets
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EC number: 700-757-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 November 2001 - 30 January 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1224866-76-5
- Cas Number:
- 1224866-76-5
- IUPAC Name:
- 1224866-76-5
- Details on test material:
- - Name of test material (as cited in study report): Glypho
- Substance type: pure active substance
- Physical state: liquid
- Stability under test conditions: Stable
- Storage condition of test material: Refrigeration at ca. 4°C
- Other: bottles not to be filled > 90%, oxygen inhibits polymerisation
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 and 100 mg/L (nominal)
- Sampling method: Duplicate samples taken from control (0 mg/L) and 100 mg/L just prior to addition of daphnids (time 0). The 100 mg/L test vessels were pooled after test conclusion (time 48) before duplicate sampling. Only one of each control duplicate was analyzed for time 0.
- Sample storage conditions before analysis: Samples frozen at ca -20 °C until analysis
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 31 mg test substance was dissolved completely in 300 mL test water by ultrasonic treatment for 10 minutes and intense stirring for 3 hours at room temperature in the dark.
The test medium was prepared just before introduction of the daphnids (start of the test).
- Controls: Blank (no test substance) only.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Slight turbidity noted throughout test period
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain: Daphnia magna Straus
- Source: In-house stock of a clone originally obtained from University of Sheffield, UK
- Age at study initiation (mean and range, SD): 6-24 hours, not first brood progeny
- Feeding during test: None
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 21 - 22 °C
- pH:
- 6.9 - 8.0
- Dissolved oxygen:
- 8.0 - 8.3
- Salinity:
- NA
- Nominal and measured concentrations:
- Nominal: 0 and 100 mg/L
Measured:- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass beakers filled with 50 mL test medium
- Aeration: No, medium aerated to oxygen saturation prior to test
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): Two
- No. of vessels per control (replicates): Two
- Biomass loading rate: < 1 daphnid / 2 mL test medium
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Analytical grade salts reconstituted in purified water.
Composition:
CaCI2 x 2H2O, 294.0 mg/L
MgSO4 X 7H2O, 123.0 mg/L
NaHCO3, 65.0 mg/L
KCI, 5.8 mg/L
- Alkalinity: 0.8 mmol
- Ca/mg ratio: 4:1
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light : 8 h dark, 30 minute transition period
- Light intensity: Between 200 and 1200 Lux during light periods
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
immobility after 24 and 48 hours of exposure (immobility: no swimming within 15 seconds after gentle agitation of the test beaker)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: None, limit test
- Range finding study: Yes, but results not reported. Test substance concentrations were stable in the rangefinding test.- Reference substance (positive control):
- not specified
- Remarks:
- Potassium dichromate is tested as a positive control at least once a year to demonstrate satisfactory test conditions.
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: No immobilized daphnids in controls or at 100 mg/L
- Details on results:
- - Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: A slight turbidity was observed throughout the test period
- Effect concentrations exceeding solubility of substance in test medium: Water solubililty of test substance is 63 g/L. Analytical recovery of the test substance from the Daphnia test medium was 93% of nominal.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- <10% immobilization in controls, > 3 mg DO/L throughout testing period
- Conclusions:
- The 48-hour EC50 (immobilisation) of the test substance to Daphnia magna is >100 mg/L. No immobilisation was seen at this concentration
- Executive summary:
Toxicity of the test substance to Daphnia magna was assessed according to OECD TG203 under GLP criteria. A slight turbidity was observed throughout the test period, however analytical recovery of the test substance was 93% of nominal. No immobilisation of controls or test subjects was observed. The 48-hour EC50 (immobilisation) of the test substance to Daphnia magna is >100 mg/L.
The test was conducted according to internationally accepted test guidelines and under GLP criteria. It is considered reliable without restriction and is suitable for Risk Assessment, Classification & Labelling, and PBT Assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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