2,4-dichloro-α,α,α-trifluorotoluene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October 26 - December 22, 1998

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dichloro-α,α,α-trifluorotoluene

EC Number:

206-278-1

EC Name:

2,4-dichloro-α,α,α-trifluorotoluene

Cas Number:

320-60-5

Molecular formula:

C7H3Cl2F3

IUPAC Name:

2,4-dichloro-1-(trifluoromethyl)benzene

Details on test material:

- Name of test material (as cited in study report): 2,4 Dichloro Benzotrifluoride
- Substance type: insoluble in water
- Physical state: liquid
- Purity test date: 99.8%
- Lot/batch No.: 950020
- Expiration date of the lot/batch: May 1999
- Storage condition of test material: room temperature, away from direct sunlight

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: University of Sheffield UK
- Age at study initiation (mean and range, SD): 6-24 hours old, not first brood progeny
- Amount: 20 daphnids, divided into two groups

The daphnids were not fed and the test media were not areated during the test period.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.5 mmol/l (reconstitued water)

Test temperature:

water temperature: 20-21 °C

pH:

8.0 - 8.1

Dissolved oxygen:

8.3 mg/l

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.18 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 0.18 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Details on results:

No toxic effect up to the highest concentration which could be dissolved in the test water.
The mean analytically determined test concentration in the samples from the freshly prepared test medium amounted to 0.17 and 0.19 mg/l at the start and at the end of the test. All biological results are related to the total mean measured test item concentration of 0.18 mg/l (calculated as the average over all measurements in the undiluted filtrate during the test period).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test item had no toxic effect on the daphnids up to highest test item concentration, which could be dissolved or very finely dispersed in the test water.