Benzenamine, 3-(4-methyl-1H-imidazol-1-yl)-5-(trifluoromethyl)-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16 February - 17 March 2005

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

641 571 - 1 1-1

IUPAC Name:

641 571 - 1 1-1

Details on test material:

- Name of test material (as cited in study report):AMN 107 B6
- - Physical state:solid
- Analytical purity:100.3 %
-- Lot/batch No.:CHAD0409
- Expiration date of the lot/batch:30 June 2005
- - Stability under test conditions:Stable
- Storage condition of test material:At room temperature in the dark

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

22 mg/L

Based on:

test mat.

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

6

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

In conclusion, AMN107 B6 was not readily biodegradable under the conditions of the modified
Sturm test presently performed.

Executive summary:

Determination of 'ready' biodegradability: carbon dioxide (CO2) evolution test (modified Sturm test) with AMNI 07 B6. The study procedure was based on EEC directive 92/69, C.4-C, December '1 992, OECD guideline No. 301 B July 17, 1992 and IS0 Standard 9439 (1999). AMN107 B6 was a faintly beige powder with a purity of 100.3%. AMN107 B6 was tested in duplicate at 44 mg per 2 litres, corresponding to 12 mg TOCII. The organic carbon content was based on the molecular formula. The study consisted of six bottles: - 2 blank controls (no test material), - 2 test bottles (AMN 107 B6, 22 mgll), - 1 positive control (sodium acetate, 40 mgll) and - 1 toxicity control (AMN107 B6, 22 mg/l; plus sodium acetate, 40 mgll). Since AMN 107 B6 was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/l, weighed amounts of AMN'I 07 B6 were added to the test bottles containing medium with microbial organisms and mineral components. After vigorous mixing the resulting suspension was added quantitatively to the test medium. The test solutions were continuously stirred during the test, to ensure optimal contact between the test substance and the test organisms. The relative degradation values calculated from the measurements performed during the test period revealed no significant degradation of AMN107 B6. In the toxicity control, AMN 107 B6 was found not to inhibit microbial activity. Since all criteria for acceptability of the test were met, this study was considered to be valid. In conclusion, AMN107 B6 was not readily biodegradable under the conditions of the modified Sturm test presently performed.