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EC number: 406-040-9 | CAS number: 125643-61-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1999-12-01 to 2000-01-07 (experimental phase)
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 406-040-9
- EC Name:
- -
- Cas Number:
- 125643-61-0
- Molecular formula:
- C17 H25 O3 R, Where R = (C7 H15) or (C8 H17) or (C9 H19)
- IUPAC Name:
- C7-9-(branched)-alkyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propanoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- For the analytical measurements, duplicate samples were taken from the freshly prepared undiluted emulsion filtrate at each test medium preparation date. Since the water solubility limit of the test item was below the limit of quantification, no samples were taken from the dilutions of the filtrate.
The duplicate samples from the start of the test were analyzed immediately after preparation. The duplicate samples from the other sampling dates were deep-frozen (at about -20 °C) immediately after sampling and were analyzed after the end of the test.
Storage
Series I: The samples were analysed after the sampling procedure (treatment samples and biological control samples of sampling day 0).
Series II: The samples were stored deep frozen and protected from light until analysis was performed (treatment samples and biological control samples of sampling days 2 to 19).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: According to a pre-experiment (without GLP) at RCC the solubility limit of the test item in water respectively test water is < 0.03 mg/l.
Therefore, a filtrate of a supersaturated emulsion of the test item and dilutions of the filtrate were prepared. The undiluted filtrate and the dilutions 1:3.2, 1:10, 1:32 and 1:100 were tested. Additionally, a control was tested in parallel (test water without test item).
Prior to each test medium renewal, the test media were freshly prepared as follows:
A supersaturated emulsion with a loading rate of 100 mg/l was prepared by weighing 250 mg (range: 250 - 251 mg) of the test item into 1000 ml test water. This mixture was treated by ultrasonification for 15 minutes and was made up to 2500 ml test water. No auxiliary solvent or emulsifier was used. Then, the mixture was stirred for 6.5 days at room temperature in the dark. The long stirring period was chosen to dissolve a maximum of the test item in the test water. After stirring, the undissolved fraction of the test item was allowed to separate from the water phase for another half a day. After separation a part of the test item was swimming at the water-surface (oily film), while a part was homogeneously distributed in the water phase. This supersaturated emulsion was separated from the oily film by sucking it of with a Teflon-tube and was filtered through a membrane filter (Schleicher & Schuell, Type NC45, pore size 0.45 µm).
The undiluted filtrate of the supersaturated emulsion with the maximum concentration of dissolved test item was used as the highest concentrated test medium. Additionally, adequate volumes of the filtrate were diluted with test water for the preparation of test media with lower test item concentrations. No additional dilution step was inserted. In this way the following treatments were prepared: the undiluted filtrate of the emulsion with a loading rate of 100 mg/l and the dilutions 1:3.2, 1:10, 1:32 and 1:100 of the filtrate (1 part of the filtrate added to 2.2, 9, 31, and 99 parts of test water, respectively).
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: in house culture, originally supplied by the University of Sheffield/UK in 1992, defined from the supplier as clone 5. Since this date, the clone is bred in the laboratories of RCC in reconstituted water of the quality identical to the water quality used in the tests (regarding pH, main ions, and total hardness) and under temperature and light conditions identical to those of the tests.
- Age at study initiation (mean and range, SD): < 24h
- Method of breeding: parthenogenetic reproduction
- Feeding during test: yes
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- 250 mg/L CaCO3
- Test temperature:
- 21-21°C
- pH:
- 7.6-8.0
- Dissolved oxygen:
- test start: 8.6-8.9 mg/L
test end: 8.1-8.6 mg/L - Nominal and measured concentrations:
- control, dilution 1:100; 1:32; 1:10, 1:3.2, undiluted filtrate
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glas beaker nominal volume 100 mL, covered with glas plates
- Fill volume: approx. 80 mL
- Renewal of test solution: on Days 2, 5, 7, 9, 12, 14, 16 and 19 of the exposure period
- No. of organisms per vessel: 1 parent animal
- No. of vessels per concentration (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic medium M7
- After preparation the M7 medium was aerated until saturation with oxygen is reached.
OTHER TEST CONDITIONS
- Photoperiod: Artificial Iight, day/night-rhythm = 16 / 8 hours
- Light intensity: about 300-800 lux
EFFECT PARAMETERS MEASURED
- Reproduction and mortality on Days 2, 5, 7, 9, 12, 14, 16 and 19 of the exposure period - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrations
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- <= 0.01 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: dilution 1:3.2, NOEC calculated by assuming a water solubility of < 0.03 mg/L
Any other information on results incl. tables
At the start of the test medium renewal periods, the test item concentration in the analyzed test medium (the undiluted filtrate of the supersaturated emulsion) was below the limit of quantification of 0.03 mg/l. Therefore, all reported biological results are related to the 'undiluted filtrate' and its 'dilutions', respectively.
In the control and at the dilutions from 1:100 to 1:3.2 the survival rate and reproduction rate of the test animals at the end of the test was 100%.
Taking into account the survival rates and the reproduction rates of the test animals, the dilution 1:3.2 was the highest treatment of the test substance tested without toxic effects after the exposure period of 21 days (21 -day NOEC). Thus, the 21 -day NOEC was approximately threefold below the water solubility of the test substance. The undiluted filtrate was the lowest treatment tested with toxic effects (21 -day LOEC) due to the significant mortality rate and due to the statistically significantly reduced mean reproduction rate of Daphnia magna at the water solubility limit of the test item.
No remarkable observations were made concerning the appearance of the test media. All test media were clear solutions throughout the test period.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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