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Diss Factsheets
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EC number: 941-484-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of 3-methyl-1-octylpyridinium chloride and 4-methyl-1-octylpyridinium chloride
- EC Number:
- 941-484-1
- Molecular formula:
- not possible
- IUPAC Name:
- Reaction mass of 3-methyl-1-octylpyridinium chloride and 4-methyl-1-octylpyridinium chloride
- Test material form:
- liquid: viscous
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method:
All concentration levels and the control were analytically verified via the test item LC-MS/MS at the start (0 h) and at the end of the exposure (48 h).
At the start of the exposure (0 h), sampling was carried out after preparation of the test concentrations. At the end of the exposure (48 h), samples were taken directly from the test vessels.
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Agitation
- Controls: Dilution water without test item tested under the same conditions as the test groups
- Evidence of undissolved material: test item is completely dissolved
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Method of breeding: In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 2°C, in an incubator, 16 h illumination, light intensity of max. 20 µE x m-2 x s-1.
- Feeding during test
The culture daphnids are fed at least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata and Desmodesmus subspicatus, with an algae cell density of > 10^6 cells/mL. The algae are cultured at the test facility.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- total hardness: 260 [mg CaCO3/L]
- Test temperature:
- 18 - 22°C, constant within ± 1°C
- pH:
- 7.03 - 7.15
- Nominal and measured concentrations:
- The measured test item concentrations were all within ± 20 % of the nominal concentrations. This indicates that the test item concentrations were successfully maintained for the duration of the test.
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: all glass test aquaria of 50 mL volume filled with a20 mL test medium
- Aeration: no
- No. of organisms per vessel: 4
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: double distilled water
- Total Hardness [mg CaCO3/L]: 260
- Conductivity: 662 µS/cm
- pH-Value: 7.63
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 h light/dark cycle
- Light intensity: Diffuse light, light intensity of max. 20 µE x m-2 x s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobilisation was monitored one and two days after exposure
TEST CONCENTRATIONS
- Spacing factor for test concentrations: The measured test item concentrations were all within ± 20 % of the nominal concentrations. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate p.a. (SIGMA)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.446 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 0.421 – 0.472 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: all daphnids were mobile in the control during the 2 days of exposure.
- Any observations that might cause a difference between measured and nominal values: the test substance was soluble in medium, the test concentrations were present as a solution. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50/LC50: EC50 (24 h) = 2.01 mg/L
Any other information on results incl. tables
PYRIDINBASE Bulk SPECIAL Nominal test item concentration [mg/L] |
IMMOBILISATION [%] | |||||||||
24 h | 48 h | |||||||||
Replicates | Replicates | |||||||||
1. | 2. | 3. | 4. | MV | 1. | 2. | 3. | 4. | MV | |
2.00 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
1.00 | 80 | 40 | 40 | 60 | 55 | 100 | 100 | 100 | 100 | 100 |
0.500 | 40 | 40 | 0 | 20 | 25 | 100 | 60 | 60 | 60 | 70 |
0.250 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
0.125 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the nominal concentrations of the test item PYRIDINBASE Bulk SPECIAL, the 48 h-EC50 for Daphnia magna was 0.446 mg/L (95 % confidence limits: 0.421 – 0.472 mg/L). The NOEC after 48 h was 0.250 mg/L. The LOEC after 48 h was 0.500 mg/L.
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