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EC number: 205-992-0 | CAS number: 274-09-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Predates implementation of GLP and/or development of study guidelines, restrictions in reporting, acceptable with restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenic activity of some centrally active aromatic amines in salmonella typhimurium.
- Author:
- White T.J.
- Year:
- 1 977
- Bibliographic source:
- Mutation Research, Elsevier/North-Holland Biomedical Press, 1977, Vol. 56, No. 2, pg. 199-202
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The method of testing for mutagenic activity was that of Ames et al, Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test, Mutation Res., 31 (1975) 347-364.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1,3-benzodioxolane
- EC Number:
- 205-992-0
- EC Name:
- 1,3-benzodioxolane
- Cas Number:
- 274-09-9
- Molecular formula:
- C7H6O2
- IUPAC Name:
- 2H-1,3-benzodioxole
- Details on test material:
- Name: 1,2-methylenedioxybenzene
Source: Aldrich Chemical Co.
Compound was pure by the criteria of melting point and elemental analysis
Constituent 1
Method
- Target gene:
- Histidine
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1538
- Species / strain / cell type:
- S. typhimurium, other: TA 100, TA 1537, TA 98
- Metabolic activation:
- with and without
- Metabolic activation system:
- Phenobarbital, Aroclor 1254 and 3-methyl-cholanthrene induced rat liver S9 mix.
- Test concentrations with justification for top dose:
- 0.02, 0.2, 2.0, 20.0, 200, 1000 and 5000 µg/plate
- Vehicle / solvent:
- Dimethyl sulfoxide (DMSO, spectrophotometric grade)
Controlsopen allclose all
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- This strain was only used to test the activity of the S-9 preparation.
- Positive control substance:
- other: 2,5-diaminoanisole and benzidine
- Remarks:
- strain TA 1538 with metabolic activation
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: daunomycin
- Remarks:
- Strain TA 98 without metabolic activation
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: N-methyl-N'-nitro-N-nitrosoguanidine
- Remarks:
- Strain TA 100 without metabolic activation
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Strain TA 1537 without metabolic activation
- Details on test system and experimental conditions:
- Revertant his+ colonies were counted after incubation in the dark at 37°C for 48 hours.
Sterility controls were performed for S9 mix, DMSO and test compound.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with
- Genotoxicity:
- not specified
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium, other: TA 1537, TA 98, TA 100
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- S. typhimurium, other: TA 1537, TA 98, TA 100
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- No difference in colony counts could be detected over a dose range of 0.02 - 5000 µg when the test compound and control plates were compared in strains TA 1537, TA 98 and TA 100. Microscopic examination of the plates revealed that an adequate bacterial lawn was present.
Sterility controls of S9 mix, DMSO and test compound were negative. The positive control substances 9-aminoacridine, N-methyl-N'-nitro-N-nitrosoguanidine and daunomycin did produce substantial increases in the number of revertants per plate in strains TA 1537, TA 100 and TA 98, respectively, in the absence of liver homogenate. The activity of the S9 preparation was demonstrated by an increase in the mutagenic activity of benzidine (50µg) or 2,5-diaminoanisole (50µg) in strain TA 1538.
In spite of the sensitivity of the test method and the prescence of the liver microsomal activation system no mutagenic activity could be detected in most of the compounds tested. This may be due to the absence of the appropriate metabolic activity, since a urinary metabolite of safrole is mutagenic in the Salmonella test whereas safrole (with of without Aroclor-induced rat liver homogenate) is not mutagenic. Although safrole may be activated to a carcinogen by liver microsomal enzymes of the type induced by 3-methylcholanthrene, the test compound was not activated to mutagens by liver homogenates prepared from rats induced with phenobarbital, Aroclor of 3-methyl-cholanthrene. - Remarks on result:
- other: strains TA 1537, TA 100 and TA 98 were tested
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results:
negative
No mutagenic activity was observed for 1,2-methylenedioxybenzene over a dose range of 0.02 - 5000µg. - Executive summary:
In an Ames test 1,2-methylenedioxybenzene in DMSO was tested at 0.02, 0.2, 2.0, 20.0, 200, 1000 and 5000 µg/plate in Salmonella typhimurium strains TA 1537, TA 98 and TA 100 with and without rat liver microsomal activation. Revertant his+ colonies were counted after incubation in the dark at 37°C for 48 hours. No difference in colony counts could be detected over a dose range of 0.02 - 5000 µg when compound and control plates were compared.
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