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EC number: 227-290-3 | CAS number: 5766-67-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data on batch no. is given; no data on the composition of the test compound. Limited reported study comparable to guideline/standard (max reliability score can be 2).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 798.4500 (Acute Eye Irritation)
- Principles of method if other than guideline:
- Comparable to OECD 405.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Ethylenediaminetetraacetonitrile
- EC Number:
- 227-290-3
- EC Name:
- Ethylenediaminetetraacetonitrile
- Cas Number:
- 5766-67-6
- Molecular formula:
- C10H12N6
- IUPAC Name:
- 2-({2-[bis(cyanomethyl)amino]ethyl}(cyanomethyl)amino)acetonitrile
- Details on test material:
- Name of test compound: ethylenediaminetetraacetonitrile
Batch no.: 1036-37-A
Appearance: white solid
Storage: room temperature
Constituent 1
Test animals / tissue source
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Eastern Rabbit Breeding Lab, Taunton, MA, USA
- Age at study initiation: 10-12 weeks
- Weight at study initiation: 2-3 kg
- Housing: individually in suspended stainless steel cages
- Diet (e.g. ad libitum): ad lib
- Water (e.g. ad libitum): ad lib
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20±1
- Humidity (%): 30-70
- Air changes (per hr): 10-13
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: From: 17 To: 20 February 1993
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): it had been indicated that 0.1 mL was used after grinding to a fine powder.
- Concentration (if solution): undiluted
VEHICLE: not used - Duration of treatment / exposure:
- single application
- Observation period (in vivo):
- 1, 24, 48 and 72 h
- Number of animals or in vitro replicates:
- 6
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): 0.9% NaCl for injection
- Time after start of exposure: 24 h
SCORING SYSTEM:
CORNEA
Degree of Opacity (most dense area used)
No opacity 0
Scattered or diffuse areas, details of iris clearly visible 1
Easily discernible translucent areas, details of iris slightly obscured 2
Nacreous areas, no details of iris visible, size of pupil barely discernible 3
Opaque cornea, iris not discernible 4
IRIS
Normal 0
Folds above normal, congestion, swelling, circumcorneal injection
(any or all of these or combination of any thereof), iris still reacting to light
(sluggish reaction is positive) 1
No reaction to light, haemorrhage, gross destruction (any or all of these) 2
CONJUNCTIVAE
Redness (refers to palpebral and bulbar conjunctivae excluding cornea and iris)
Vessels normal 0
Vessels definitely injected above normal 1
More diffuse, deeper crimson red, indiviual vessels not easily discernible 2
Diffuse beefy red 3
Chemosis
No swelling 0
Any swelling above normal (includes nictitating membrane) 1
Obvious swelling with partial eversion of lids 2
Swelling with lids about half closed 3
Swelling with lids more than half closed 4
TOOL USED TO ASSESS SCORE: fluorescein and han held slit-lamp
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- other: 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable as there were no changes
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- other: 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: not applicable as there were no changes
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- other: 24/48/72 h
- Score:
- 0.06
- Max. score:
- 3
- Reversibility:
- fully reversible within: 2 days
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- other: 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable as there were no changes
- Irritant / corrosive response data:
- Slight redness was observed in 4/6 and moderate redness in 2/6 animals 1 h after exposure. Slight redness was still seen in one
animal 24 h after application. Slight discharge was seen in 3/6 animals. All treated eyes appeared normal 2 days after application. - Other effects:
- No other signs of toxicity were noted; all animals exhibited BW gain.
Any other information on results incl. tables
Summary of ocular changes
Anim. No. |
Effect |
Hours |
Days after application |
Mean score cornea Days 1/2/3 |
Mean score iritis Days 1/2/3 |
Mean score redness Days 1/2/3 |
Mean score chemosis Days 1/2/3 |
||||
1 |
1 |
2 |
3 |
7 |
21 |
||||||
30586 |
Cornea Iris Redness Chemosis |
0 0 1 0 |
0 0 0 0 |
0 0 0 0 |
0 0 0 0 |
- - - - |
- - - - |
0 |
0 |
0 |
0 |
30587 |
Cornea Iris Redness Chemosis |
0* 0 1 0 |
0* 0 0 0 |
0* 0 0 0 |
0 0 0 0 |
- - - - |
- - - - |
0 |
0 |
0 |
0 |
30588 |
Cornea Iris Redness Chemosis |
0 0 2 0 |
0* 0 1 0 |
0 0 0 0 |
0 0 0 0 |
- - - - |
- - - - |
0 |
0 |
0.33 |
0 |
30589 |
Cornea Iris Redness Chemosis |
0 0 1 0 |
0 0 0 0 |
0 0 0 0 |
0 0 0 0 |
- - - - |
- - - - |
0 |
0 |
0 |
0 |
30590 |
Cornea Iris Redness Chemosis |
0* 0 2 0 |
0 0 0 0 |
0 0 0 0 |
0 0 0 0 |
- - - - |
- - - - |
0 |
0 |
0 |
0 |
30591 |
Cornea Iris Redness Chemosis |
0 0 1 0 |
0 0 0 0 |
0 0 0 0 |
0 0 0 0 |
- - - - |
- - - - |
0 |
0 |
0 |
0 |
Mean all anim. |
0 |
0 |
0.06 |
0 |
* discharge (any amount different from normal)
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: OECD GHS
- Conclusions:
- Although slight to moderate redness was observed 1 h and 24 h after application, there is no need for classification according to
OECD-GHS. - Executive summary:
A study was performed to assess the irritancy potential of the test material, Ethylenediaminetetraacetonitrile, to the eye of the New Zealand White rabbit. The method used essentially followed that described in the OECD Guidelines for Testing of Chemicals No. 405. A single application of the test material to the eye of six rabbits produced minimal to moderate redness and slight discharge. All treated eyes appeared normal 48 hours after treatment. Although the test material induced minimal irritation, there is no need for classification as an irritant according to OECD-GHS.
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