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EC number: 228-985-4 | CAS number: 6386-38-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994-10-25 to 1995-03-24
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP-study. Guideline from 1986.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.20 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate
- EC Number:
- 228-985-4
- EC Name:
- Methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate
- Cas Number:
- 6386-38-5
- Molecular formula:
- C18H28O3
- IUPAC Name:
- methyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propanoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
Day 0: from all test solutions including the controls
Day 2: from the 'old' solutions at 0.16, 0.56 and 2.2 mg/L
Days 6 and 14: from 0.56, 1.0 and 2.2 mg/L
Day 21: from all test solutions including the controls
- Volume: 10 mL
- Sample storage conditions before analysis: under deep frozen conditions
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: First, stock solutions were prepared in methanol (Merck, extra pure) at a concentration of 25 g/L on a weekly basis. These stocks were used to prepare fresh stock solutions in methanol (Merck, extra pure) at the start of the test and at each renewal. These stocks contained test substance concentration of a factor of 10E04 greater than the final test concentrations in M4 medium to provide a methanol concentration of 0.1 mL/L in all test solutions. All test solutions were clear. There was no indication of precipitation of the test materia
1. Test medium without test substance or any other additives (0 mg/L),
2. Test medium without test substance but with the additive used in the treatment of the stock solutions, i.e. methanol (0.1 mL/L).
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (Crustacea, Cladocera) (Straus, 1820)
- Age of parental stock (mean and range, SD): < 24 hours
- Feeding during test: yes
- Food type: a suspension of fresh water algae
- Amount: The ration was 1.10E06 cells/ml/day
- Frequency: daily
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- 19.5 - 21.5 °C
- pH:
- 7.6 - 8.7
- Dissolved oxygen:
- 7.0 - 9.8 mg/L
- Nominal and measured concentrations:
- nominal: 0.16, 0.30, 0,56, 1,0 and 2.2 mg/L
measured: 0.123, 0.173, 0.367, 0.572, 1.143 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml (8 x 04 cm), all-glass covered with a perspex plate.
- No. of organisms per vessel: 1 neonate
- No. of vessels per concentration (replicates): 10 vessels per concentration
TEST MEDIUM / WATER PARAMETERS
- M 4 in accordance to the guideline
OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily
- Light intensity: 600 lux
VEHICLE CONTROL PERFORMED: yes - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 1.8 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- immobilisation
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.97 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- immobilisation
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 1.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: 0.9 - 1.2
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.59 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: 0.54 - 0.65
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: overall
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.367 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: overall
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Based on Dunns Multiple Comparison-Kruskal-Wallis test (P=0.05)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.173 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Based on Dunns Multiple Comparison-Kruskal-Wallis test (P=0.05)
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 0.18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: 0.00 - 0.33 mg/L
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 0.14 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: 0.08 - 0.21 mg/L
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Based on Williams' Test (P=0.05)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.123 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Based on Williams' Test (P=0.05)
- Details on results:
- - Mortality of parent animals:
During the main part of the test period, the EC50 for mobility remained > the highest test concentration which corresponded with the maximum solubility of test item. On day 20 a total of 6 out of the 10 parental Daphnia had died. At lower concentrations, mortality of parental Daphnia remained at or below 20 % during the total test period of 21 days, with exception of those exposed to 0.56 mg/l. Here one Daphnia was lost at the renewal on day 12. Therefore, the final mortality rate was 22 %, I.e. 2 out of 9 parental Daphnia. - Reported statistics and error estimates:
- The reproduction values of the two control groups (blank and solvent) were compared with the use of the t-test. When no statistical difference was found between these two control groups, their values were combined and the reproduction values of the treated groups were statistically compared with those of the combined control groups. For each concentration the results of reproduction were tested for normality and for homogeneity of variance. Furthermore, these data were statistically tested using a mean comparison test (Williams' Test) and a multiple comparison test (Dunns Multiple Comparison - Kruskal-Wallis test). The overall threshold level of effect and the overall NOEC were determined on basis of these statistics. The EC50 (immobilisation) and the EC50 (reproduction) were calculated. Additionally an EC25 and EC10 were calculated for reproduction.
The mortality of the parental daphnids in the controls did not exceed 20%. The average cumulative number of young per female in the controls after 21 days was > 60 (blank: 161 + 20, solvent: 164 + 42).
Applicant's summary and conclusion
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