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Diss Factsheets
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EC number: 202-499-2 | CAS number: 96-32-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study was carried out under at a certified GLP laboratory using the OECD Guidelines for the Testing of Chemicals No. 423 “Acute Oral Toxicity – Acute Toxic Class Method” (adopted 17 December 2001) and Method B1 tris Acute Toxicity (Oral) of Commission Directive 2004/73/EC guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- yes
Test material
- Reference substance name:
- Methyl bromoacetate
- EC Number:
- 202-499-2
- EC Name:
- Methyl bromoacetate
- Cas Number:
- 96-32-2
- Molecular formula:
- C3H5BrO2
- IUPAC Name:
- methyl 2-bromoacetate
- Test material form:
- other: Liquid
- Details on test material:
- Material from production 98% min pure
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River (UK) Ltd, Margate, Kent, UK
- Age at study initiation: eight to twelve weeks
- Weight at study initiation: 195 - 205 g
- Fasting period before study: overnight
- Housing: suspended solid-floor polypropylene cages furnished with woodflakes
- Diet (e.g. ad libitum): free access to food
- Water (e.g. ad libitum): free access to mains drinking water
- Acclimation period: at least five days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 to 25°C
- Humidity (%): 30 to 70%
- Air changes (per hr): at least fifteen
- Photoperiod (hrs dark / hrs light): twelve hours continuous light and twelve hours darkness
IN-LIFE DATES: From: To:
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- arachis oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 30 mg/ml & 5 mg/ml
- Amount of vehicle (if gavage): 10 ml/Kg
- Justification for choice of vehicle: test material did not dissolve/suspend in distilled water
- Lot/batch no. (if required):
- Purity:
MAXIMUM DOSE VOLUME APPLIED: 10 mg/ml
- Rationale for the selection of the starting dose: absence of data regarding the toxicity of the test material - Doses:
- Group 1 - 300 mg/kg
Group 2 - 50 mg/Kg
Group 3 - 50 mg/Kg - No. of animals per sex per dose:
- 3
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: The animals were observed for deaths or overt signs of toxicity ½, 1, 2 and 4 hours after dosing
and subsequently once daily for up to fourteen days. Individual bodyweights were recorded prior to dosing and seven and fourteen days after
treatment or at death.
- Necropsy of survivors performed: yes
Results and discussion
- Preliminary study:
- The acute oral median lethal dose (LD50) of the test material in the female Sprague-Dawley CD strain rat was estimated to be in the range of 50 - 200 mg/kg bodyweight
Effect levels
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 50 - < 300 mg/kg bw
- Based on:
- test mat.
- Mortality:
- Animals treated at a dose level of 300 mg/kg were found dead during the day of dosing or one day after dosing. There were no deaths noted at a dose level of 50 mg/kg
- Clinical signs:
- other: Signs of systemic toxicity noted in animals treated at a dose level of 300 mg/kg were hunched posture, lethargy, decreased respiratory rate, laboured respiration, ataxia, pilo-erection, diarrhoea, diuresis and tiptoe gait. There were no signs of systemic
- Gross pathology:
- Abnormalities noted at necropsy of animals that died during the study were haemorrhagic or
abnormally red lungs, dark liver or patchy pallor of the liver, dark kidneys, haemorrhage of the
gastric mucosa and epithelial sloughing of the non-glandular region of the stomach. No
abnormalities were noted at necropsy of animals that were killed at the end of the study.
Applicant's summary and conclusion
- Interpretation of results:
- Toxicity Category II
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- LD50 - 50 - 200 mg/Kg
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