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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
not specified
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
N-[4-(aminocarbonyl)phenyl]-4-nitrobenzamide
EC Number:
298-791-2
EC Name:
N-[4-(aminocarbonyl)phenyl]-4-nitrobenzamide
Cas Number:
93839-21-5
Molecular formula:
C14H11N3O4
IUPAC Name:
N-(4-carbamoylphenyl)-4-nitrobenzamide
Details on test material:
purity: 99+-2% (m/m)

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
source: waster water treatment plant Frankfurt-Sindlingen

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Post exposure observation period:
no

Test conditions

Test temperature:
19.3 - 20.6 °C
pH:
7.0 - 7.1
Nominal and measured concentrations:
500 mg/l and 1000 mg/L nominal
Details on test conditions:
For each concentration, two replicates were performed (A) and (B)
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
Concentration respiration
mg/L inhibition %
1000 - 2.0
1000 - 2.0
500 7.4
500 7.4
Results with reference substance (positive control):
3,5-Dichlorophenol
Concentration respiration
mg/L inhibition %
5 22.8
20 66.9
30 71.3

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 of N-(4-carbamoylphenyl)-4-nitrobenzamide for bacteria toxicity is > 1000 mg/L
Executive summary:

N-(4-carbamoylphenyl)-4-nitrobenzamide was tested for toxicity to bacteria according to OECD 209 (Activated Sludge, Respiration Inhibition Test). Two concentrations (500 mg/L and 1000 mg/L) were used in replicates. The EC50 of N-(4-carbamoylphenyl)-4-nitrobenzamide for bacteria toxicity is > 1000 mg/L.

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