Benzoyl chloride, 3-[[[2,3-bis(acetyloxy)propyl]amino]carbonyl]-2,4,6-triiodo-5-[(2-methoxyacetyl)amino]-

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Apr 1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

Version / remarks:

Mar 1991

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

rac-3-(2,3-Diacetoxypropylcarbamoyl)-2,4,6-triiodo-5-methoxyacetylaminobenzoyl chloride

EC Number:

604-759-4

Cas Number:

150928-21-5

Molecular formula:

C18 H18 Cl I3 N2 O8

IUPAC Name:

rac-3-(2,3-Diacetoxypropylcarbamoyl)-2,4,6-triiodo-5-methoxyacetylaminobenzoyl chloride

Sampling and analysis

Analytical monitoring:

yes

Remarks:

DOC

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: For the stock solution 5 g of ZK 91.445 were suspended in 500 mL of sterile demineralized water. This solution was used for the highest concentration after ultrasonification and filtration. For the lower test concentrations, the stock solution was diluted 1:2 and 1:4. 80 mL of the respective solution were filled into the respective test vessel. In the filtered stock solution a DOC value of 136 mg/L was found corresponding to 523 mg/L ZK 91.445. Thus, the DOC measurements showed that only approximately 5% of the nominal concentration was dissolved in aqueous solution.
- Eluate: no
- Differential loading: np
- Controls: yes, test medium control

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Source: obtained from the "Deutsche Sammlung für Mikroorganismen, Göttingen", Germany
- Cultivation: ad kept on slant agar containing a nutrient solution at approximately 25°C. During culturing the microorganisms were transferred weekly.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Test conditions

Test temperature:

25 °C

Nominal and measured concentrations:

nominal: control, 131, 262, 523 mg/L

Details on test conditions:

TEST SYSTEM
- Aeration: constanly shaken
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The turbidity of the control was measured at 0 and 16 hours, whereas the turbidity of the test concentrations was measured only after 16 hours. Additionally, measurements of the control and the test concentrations without inoculum were performed after 16 hours. All measurements were made at a wave-Iength of 436 nm.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

16 h

Dose descriptor:

other: TE/F units

Effect conc.:

> 523 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Conclusions:

No effects on growth of Pseudomonas putida.

Executive summary:

The results of the growth inhibition test with Pseudomonas putida demonstrate that a solution of ZK 91.445 up to approximately 500 mg/L has no relevant effects on the bacterial growth after 16 h of exposure. However, it has to be taken into account that the compound hydrolyses in aqueous solutions and the results reflect the exposure to the parent compound as well as to the products of hydrolysis.