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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-02-05 to 1990-02-09
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: US EPA-TSCA, 40 CFR, Part 797 Guideline 797.1300
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Purity >95%
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Supplier batch #1648
- Test type:
- static
- Water media type:
- other: Autoclaved Reverse-osmosis water
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 24°C
- pH:
- 0 hours: 7.3, 96 hours: 8.1-8.6
- Nominal and measured concentrations:
- Measured concentrations: Control; solvent control; 0.093; 0.15; 0.26; 0.64; 1.1
- Details on test conditions:
- Algal cell counts accomplished utilising a hemacytometer and an Olympus Model CHA microscope
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1.1 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Details on results:
- The multiple means test indicated no significant inhibition effect (p<0.05) on growth for any of the test concentrations of XP-2563 to Selenastrum capricornutum as compared to the controls after 96-hours. Based on the results, the 95 hour EC50 values based on cell counts were estimated to be >1.1 mg/L
- Reported statistics and error estimates:
- Initial cell counts of control and solvent control flasks result in an actual mean count of 9.4 x 10^3 cells/ml
Logarithmic phase growth confirmed at 96 hours with a mean count of 2.8 x 10^6 cells/ml in the controls, a 298-fold increase from the initial 9.4 x 10^3 cells/ml.
Growth data was subjected to a 1-way analysis of variance (ANOVA) and multiple means test (Dunnett's test). The multiple means test indicated no significant inhibition effect (p<0.05) on growth for any of the test concentrations of XP-2563 to Selenastrum capricornutum as compared to the controls after 96-hours. - Validity criteria fulfilled:
- yes
- Conclusions:
- The result of the 96-hour static algal toxicity study using Selenastrum capricornutum Printz for compound XP-2563 is an estimated 96-hour EC50 (95% C.I) of >1.1 mg/L. The no effect concentration of the compound was estimated to be 1.1 mg/L since no effects were observed in any of the test concentrations after 96-hours.
- Executive summary:
The acute toxicity of XP-2563 to Selenastrum capricornutum Printz was assessed using the methods outlined in ABC Protocol #8004-PMN. Nominal concentrations for the definitive study were a geometric series of 0.13, 0.25, 0.50, 1.0 and 2.0 mg/L. Temperature and light intensity were monitored throughout the test and were within acceptable limits. Samples were collected from the control and test levels and sent to Monsanto Company for analysis. Analysis was conducted using Monsanto Method #MSL-9864, ES-90-M-1. The mean measured values of XP-2563 were: 0.093, 0.15, 0.26, 0.64, and 1.1 mg/L. All results were based on the mean measured values.
The result of the 96-hour static algal toxicity study using Selenastrum capricornutum Printz for compound XP-2563 is an estimated 96-hour EC50 (95% C.I) of >1.1 mg/L. The no effect concentration of the compound was estimated to be 1.1 mg/L since no effects were observed in any of the test concentrations after 96 hours.
Reference
Table 1. Environmental chamber data for the 96-hour static acute algal toxicity study of XP-2563 to Selenastrum capricornutum Printz
Date |
PAR* |
Light Reading |
Temperature (°C) |
Oscillation rate (RPM’s)‡ |
|
LUX** |
Ft-c† |
||||
2-05-90 |
49 |
4083 |
379 |
24 |
100 |
2-06-90 |
50 |
4167 |
387 |
24 |
100 |
2-07-90 |
49 |
4083 |
379 |
25 |
100 |
2-08-90 |
52 |
4333 |
403 |
24 |
100 |
2-09-90 |
52 |
4333 |
403 |
24 |
100 |
* = Photosynthetically active radiation (μmols-1m-2), -measured with a LI-COR Model LI-185B Quantum/Radiometer/Photometer and LI-COR Model LI-190SB Quantum Sensor (Serial #Q 7517).
** = LUX = Light reading (PAR) / 12 (correction factor for fluorescent lighting) x 1000
† = Footcandles (ft-c_ = LUX x (0.0929)
‡ = Taken directly from the gauge on the environmental chamber.
Table 2. Measured Cell Counts for Selenastrum capriconutum Printz during the exposure to XP-2563
Mean measured concentrations (mg/L) |
Mean cell counts (3 flasks) cells/ml/10^4 |
||||
0 hr |
24 hr* |
48 hr* |
72 hr* |
96 hr* |
|
Control |
0.93 |
3.6 |
15 |
85 |
250 |
Solvent Control |
0.96 |
4.0 |
21 |
100 |
310 |
0.093 |
|
2.8 |
17 |
94 |
310 |
0.15 |
|
3.2 |
18 |
90 |
310 |
0.26 |
|
3.1 |
16 |
87 |
320 |
0.64 |
|
3.3 |
17 |
94 |
350 |
1.1 |
|
3.2 |
16 |
77 |
260 |
*= Denotes no significant (p<0.05) inhibition effect from control was identified using transformed (square root) cell counts by Dunnets’ Test. Therefore, the EC50 was estimated to be >2.0 mg/L and the no-effect level was 2.0 mg/L
Table 3. Temperature and pH measurements during the exposure of Selenastrum capricornutum Printz to XP-2563
Mean Measured Concentrations (mg/L) |
0-hour |
96-hours |
||
Parent solutions |
Rep. A |
|||
Temp (°C) |
pH |
Temp (°C) |
pH |
|
Control |
24 |
7.2 |
24 |
8.2 |
Solvent Control |
24 |
7.3 |
24 |
8.6 |
0.093 |
24 |
7.3 |
24 |
8.6 |
1.15 |
24 |
7.3 |
24 |
8.1 |
0.26 |
24 |
7.3 |
24 |
8.5 |
0.64 |
24 |
7.3 |
24 |
8.5 |
1.1 |
24 |
7.3 |
24 |
8.4 |
Temperature measured using Mercury thermometer
pH measured ising Corning 140 pH/mV Meter and Beckman 39831 Electrode
Description of key information
The 96 hour EC50 for XP-2563 in Selenastrum capricornutum Printz is estimated to be >1.1 mg/L. The no effect concentration of the compound was estimated to be 1.1 mg/L since no effects were observed in any of the test concentrations after 96 hours.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.1 mg/L
- EC10 or NOEC for freshwater algae:
- 1.1 mg/L
Additional information
The acute toxicity of XP-2563 to Selenastrum capricornutum Printz was assessed using the methods outlined in ABC Protocol #8004-PMN. Nominal concentrations for the definitive study were a geometric series of 0.13, 0.25, 0.50, 1.0 and 2.0 mg/L. Temperature and light intensity were monitored throughout the test and were within acceptable limits. Samples were collected from the control and test levels and sent to Monsanto Company for analysis. Analysis was conducted using Monsanto Method #MSL-9864, ES-90-M-1. The mean measured values of XP-2563 were: 0.093, 0.15, 0.26, 0.64, and 1.1 mg/L. All results were based on the mean measured values.
The result of the 96-hour static algal toxicity study using Selenastrum capricornutum Printz for compound XP-2563 is an estimated 96-hour EC50 (95% C.I) of >1.1 mg/L. The no effect concentration of the compound was estimated to be 1.1 mg/L since no effects were observed in any of the test concentrations after 96 hours.
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