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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.6800 (Modified Activated Sludge, Respiration Inhibition Test for Sparingly Soluble Chemicals)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Test System
Species: Activated sludge, microorganisms from a domestic waste water treatment plant.
Origin: The (controlled) activated sludge was supplied by the sewage plant for domestic sewage in Balatonfüred, Hungary on 10 July 2012 (One day before the main test.).
Preparation of Activated Sludge
Inoculum:
The coarse particles were removed by settling for 10 minutes, and the upper layer of finer solids was decanted. The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in isotonic saline solution with shaking and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension
was weighed, dried and the ratio of wet sludge to dry weight determined. Based on this ratio, calculated amount of wet sludge was suspended in isotonic saline solution to yield a concentration equivalent to 3 g per litre (on dry weight basis). The activated sludge was not used on the day of the collection but continuously aerated (2L/minute) at the test temperature for about 24 hours (1 day) and was fed once with
50 mL synthetic sewage/L activated sludge. The pH of the activated sludge inoculum was checked after
preparation and before use. The pH of the activated sludge inoculum after preparation was 7.94, before use: 7.88. The pH adjustment before use was considered as not necessary. Foaming: Difficulties can arise if foaming occurs during the incubation to the extent that the foam and the sludge solids carried on it,
are expelled from the aeration vessels. In this test the occurring foaming was not significant, thus controlling was not necessary during the incubation. - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 2.2-4.4℃
- pH:
- 7.53
- Nominal and measured concentrations:
- nominal 1000 mg test item/L
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The observed oxygen consumption rates consequently the specific respiration rates were in the range of the blank controls, no inhibitory effect (the observed slight 0.17 % “inhibition” was within the biological variability of the applied test system) of the test item was observed.
The specific respiration rates were compared with the blank control values using 2 Sample t-Test (α=0.01). No statistical significant differences were observed in the comparison with the blank control values, consequently based on the results of this study the NOEC can be statistically determined as ≥1000 mg/L.
Based on measured oxygen consumption values and calculated specific respiration rates it can be stated that the 3-hour EC10 and EC50 values of the test item are higher than 1000 mg/L.The EC50 value was determined as: EC50 > 1000 mg/L. - Results with reference substance (positive control):
- The following concentrations of the positive reference control 3,5-Dichlorophenol were tested on the same
activated sludge and under identical conditions as the test item: 2, 7 and 24.5 mg/L. Each concentration of the 3,5-Dichlorophenol reference item was tested in three parallels. In comparison to the blank controls the specific respiration rate of the activated sludge was inhibited by 3.70 % at the lowest concentration of 2 mg/L, at the nominal concentrations of 7 and 24.5 mg/L, the respiration rate was inhibited by 8.81 % and 69.0 %, respectively. - Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the performed Activated Sludge Respiration Inhibition Test, the
EC10 and EC50 values of test item was determined as higher than 1000 mg/L. Based on the
statistical evaluation in this test the NOEC was ≥1000 mg/L. - Executive summary:
In an 3-hour OECD 209 study under GLP compliance, the influence of the test item Reaction product of 2-hydroxybenzoic acid,
styrene and oxozinc on the activity of the activated sludge was evaluated by measuring the respiration rate under defined conditions.The respiration rates (total, heterotrophic and nitrification oxygen uptake rates) of samples of activated sludge fed with synthetic sewage were measured in an enclosed cell containing an oxygen electrode after a contact time of 3 hours.Based on the preliminary information (the test item caused no effect on the activated sludge inoculum), the test item Reaction
product of 2-hydroxybenzoic acid, styrene and oxozinc was investigated at the concentration of 1000 mg/L as a limit
concentration, only.The test item was investigated at one (limit) concentration level of 1000 mg/L. The observed oxygen consumption rates consequently the specific respiration rates were in the range of the blank controls, no inhibitory effect of the test item was observed.Based on measured oxygen consumption values and calculated specific respiration rates it can be stated that the 3-hour EC10 and EC50 values of the test item are higher than 1000 mg/L. The EC50 value was determined as: EC50 > 1000 mg/L. All validity criteria of the study were met.
Reference
Description of key information
Activated Sludge Respiration Inhibition Test:EC50 > 1000 mg/L(OECD209,GLP)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
In an 3-hour OECD 209 study under GLP compliance, the influence of the test item Reaction product of 2-hydroxybenzoic acid,
styrene and oxozinc on the activity of the activated sludge was evaluated by measuring the respiration rate under defined conditions.The respiration rates (total, heterotrophic and nitrification oxygen uptake rates) of samples of activated sludge fed with synthetic sewage were measured in an enclosed cell containing an oxygen electrode after a contact time of 3 hours.Based on the preliminary information (the test item caused no effect on the activated sludge inoculum), the test item Reaction
product of 2-hydroxybenzoic acid, styrene and oxozinc was investigated at the concentration of 1000 mg/L as a limit
concentration, only.The test item was investigated at one (limit) concentration level of 1000 mg/L. The observed oxygen consumption rates consequently the specific respiration rates were in the range of the blank controls, no inhibitory effect of the test item was observed.Based on measured oxygen consumption values and calculated specific respiration rates it can be stated that the 3-hour EC10 and EC50 values of the test item are higher than 1000 mg/L. The EC50 value was determined as: EC50 > 1000 mg/L. All validity criteria of the study were met.
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