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Diss Factsheets
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EC number: 446-560-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 17 May to 20 May 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: Commission Directive 88/302/EEC; Official Journal of the EG L133 Part C (1988)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Direct weighing into test flasks
- Controls: Yes, blank control
- Test concentration separation factor: 1.8 - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: mixed population of aquatic microorganisms(activated sludge)
- Name and location of sewage treatment plant where inoculum was collected: aeration tank of a domestic sewage treatment plant(Wupper area water authority)
- Method of cultivation: Stirring period of test item before start of incubation time: 16 h.
- Initial biomass concentration: 480 mg/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 +/- 2 °C
- Nominal and measured concentrations:
- 10, 18, 32, 56, 100 mg/L as nominal concentrations of the test item.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Flask
- Aeration: Permanent aeration
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2 controls
- Sludge concentration (weight of dry solids per volume): 480 mg/L suspended solids
- Nutrients provided for bacteria: Daily feed with synthetic medium
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : respiration inhibition at 3 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 44 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: The solubility was not exceeded.
- Blank controls oxygen uptake rate: 34 mg/L - Results with reference substance (positive control):
- - Results with reference substance valid? Yes, all validity criteria are fulfilled.
- Relevant effect levels: 0 % inhibition at 2.5 mg/L, 82.4 % inhibition at 40 mg/L.
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on results incl. tables'.
- Conclusions:
- EC50 (3 h): 44 mg/L
- Executive summary:
The test item showed 100 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/L. The EC50 was determined to be 44 mg/L.
Reference
Validity criteria for the measurement of the toxicity to microorganisms:
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
In a 2004 international ring test organized by ISO using activated sludge derived from domestic sewage, the EC50 of 3,5-dichlorphenol was found to lie in the range 2 mg/L to 25 mg/L for total respiration, 5 mg/L to 40 mg/L for heterotrophic respiration and 0.1 mg/L to 10 mg/L for nitrification respiration. If the EC50 of 3,5-DCP does not lie in the expected range, the test should be repeated with activated sludge from another source. | The EC 50 of the reference substance 3.5-dichlorophenol is in the range 5 - 30 mg/L, which is 5 mg/L above the range specified in the guideline. Considering the small deviation, this criterion is considered to be met. | Yes |
The test should be performed at a temperature within the range 20 ± 2°C. | The test temperature was 20 ± 2°C. | Yes |
The EC50 and, if possible, 95 % confidence limits, possibly EC20, EC80; possibly NOEC and the used statistical methods, if the EC50 cannot be determined shall be reported. | The EC50 including the 95% confidence limits are stated. The EC values were determined by probit analysis. | Yes |
Description of key information
The test item showed 100 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/L. The EC50 was determined to be 44 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 44 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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