Recyclamine R 501

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard test guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Principles of method if other than guideline:

To determine Adsorption coefficient (KOC) on soil and on sewage sludge of the test chemical using HPLC.

GLP compliance:

no

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Radiolabelling:

not specified

Test temperature:

25°C

Details on study design: HPLC method:

HPLC Procedure:
The solutions of the test substance and reference substances were prepared in solvent. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k. The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor.

Operating Conditions:
HPLC Model No. : Agilent Technologies,1260 Infinity II
Flow Rate : 1.0ml/min
Column : Zorbax Eclipse XDB-CN, 4.6*250 mm, 5µ
Mobile phase : Acetonitrile: water (55:45)
Temperature : 25°C
Detector : Diode Array Detector @210nm
Injector : Autosampler
Injector volume : 10μl
Data Acquisition software : Chem Station Open lab Control Panel

Analytical monitoring:

not specified

Details on sampling:

- Concentrations: 900 mg/l
- Sampling interval: A test chemical solution was prepared by accurately weighing 10 µL of test item and diluted with acetonitrile up to 10 ml. Thus, the test solution concentration was 900 mg/l. The pH of test substance was 7.9.

Key result

Sample No.:

#1

Type:

log Koc

Value:

4.02 dimensionless

pH:

7.9

Temp.:

25 °C

Remarks on result:

other: LogKoc value was reported to be 4.020±0.050.

Details on results (HPLC method):

The Adsorption coefficient (KOC) on soil and on sewage sludge of the test chemical was determined to be 4.020±0.050 at 25°C.

Transformation products:

not specified

Table 1:

Reference substances	tR1	tR2	Mean tR± S.D.	k'	Log k'	Log Koc
Formamide	2.795	2.789	2.792±0.004	NA	NA	NA
Acetanilide	3.259	3.259	3.259±0.000	0.167	-0.777	1.1
N,N-dinitrobenzamide	3.314	3.308	3.311±0.003	0.186	-0.731	1.4
Aniline	3.485	3.489	3.487±0.003	0.249	-0.604	2.0
1,2,3-Trichlorobenzene	4.256	4.253	4.255±0.002	0.524	-0.281	3.8
Phenanthrene	4.604	4.595	4.600±0.006	0.647	-0.189	4.1
DDT	6.010	6.015	6.013±0.004	1.153	0.062	5.0

Table 2:

Test chemical		k'	Log k'	Log Koc	Mean of Log Koc± S.D
tR1	4.691	0.682	-0.166	4.058	4.020 ±0.050
tR2	4.626	0.659	-0.181	3.987

Validity criteria fulfilled:

yes

Conclusions:

The Adsorption Coefficient of test substance was determined as per the HPLC method (OECD Guideline-121). The Log Koc value was determined to be 4.020±0.050 at 25°C. This log Koc value indicates that the test chemical has a strong sorption to soil and sediment and therefore have negligible to slow migration potential to ground water.

Executive summary:

The adsorption coefficient Koc in soil and in sewage sludge of test chemical was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. A test chemical solution was prepared by accurately weighing 10 µL of test item and diluted with acetonitrile up to 10 ml. Thus, the test solution concentration was 900 mg/l. The pH of test substance was 7.9. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k. The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor. The reference substances were chosen according to estimated Koc range of the test substance and generalized calibration graph was prepared. The reference substances were Acetanilide, N,N-dinitrobenzamide, Aniline, 1,2,3-Trichlorobenzene, Phenanthrene, and DDT having Koc value ranging from 1.1 to 5.0. The Log Koc value was determined to be 4.020±0.050 at 25°C. This log Koc value indicates that the test chemical has a strong sorption to soil and sediment and therefore have negligible to slow migration potential to ground wate.

Description of key information

The adsorption coefficient Koc in soil and in sewage sludge of test chemical was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. A test chemical solution was prepared by accurately weighing 10 µL of test item and diluted with acetonitrile up to 10 ml. Thus, the test solution concentration was 900 mg/l. The pH of test substance was 7.9. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k. The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor. The reference substances were chosen according to estimated Koc range of the test substance and generalized calibration graph was prepared. The reference substances were Acetanilide, N,N-dinitrobenzamide, Aniline, 1,2,3-Trichlorobenzene, Phenanthrene, and DDT having Koc value ranging from 1.1 to 5.0. The Log Koc value was determined to be 4.020±0.050 at 25°C. This log Koc value indicates that the test chemical has a strong sorption to soil and sediment and therefore have negligible to slow migration potential to ground wate.

Key value for chemical safety assessment

Koc at 20 °C:

10 471.28

Additional information

The adsorption coefficient Koc in soil and in sewage sludge of test chemical was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. A test chemical solution was prepared by accurately weighing 10 µL of test item and diluted with acetonitrile up to 10 ml. Thus, the test solution concentration was 900 mg/l. The pH of test substance was 7.9. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k. The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor. The reference substances were chosen according to estimated Koc range of the test substance and generalized calibration graph was prepared. The reference substances were Acetanilide, N,N-dinitrobenzamide, Aniline, 1,2,3-Trichlorobenzene, Phenanthrene, and DDT having Koc value ranging from 1.1 to 5.0. The Log Koc value was determined to be 4.020±0.050 at 25°C. This log Koc value indicates that the test chemical has a strong sorption to soil and sediment and therefore have negligible to slow migration potential to ground wate.