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EC number: 217-199-7 | CAS number: 1772-25-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2022.06.9-2022.09.21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
- Species:
- rat
- Strain:
- Wistar
- Remarks:
- WISTAR rats Crl: WI(Han) (full barrier)
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River, 97633 Sulzfeld, Germany
- Females (if applicable) nulliparous and non-pregnant: yes
- Age at study initiation: 8-9 weeks
- Weight at study initiation: 175-180g
- Fasting period before study: 16-18 hours
- Housing: Full barrier in an air-conditioned room
- Historical data: none
- Diet (e.g. ad libitum): libitum
- Water (e.g. ad libitum):libitum
- Acclimation period: at least 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18.7 – 21.4 °C
- Humidity (%): 40 – 63%
- Air changes (per hr): 10 x / hour
- Photoperiod (hrs dark / hrs light): 12 hours light, 12 hours dark
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- Based on OECD Guideline 423, Annex2
MAXIMUM DOSE VOLUME APPLIED: 2000mg/kg bw
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: the LD50 value in rats was estimated to be 1200 mg/kg bw - Doses:
- 2000, 300 mg/kg bw
- No. of animals per sex per dose:
- 3
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: every 7 days
- Necropsy of survivors performed: yes
- Clinical signs including body weight: none - Sex:
- not specified
- Dose descriptor:
- LD50 cut-off
- Effect level:
- 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- Under the conditions of the present study, a single oral application by gavage of the test item Hexane-1,3,6-tricarbonitrile to rats at a dose of 2000 mg/kg bw was associated with signs of toxicity and mortality.
Under the conditions of the present study, a single oral application by gavage of the test item Hexane-1,3,6-tricarbonitrile to rats at a dose of 300 mg/kg bw was associated with signs of toxicity but no mortality. - Body weight:
- lower than 10% body weight loss
- Remarks:
- None of the animals showed weight loss during the observation period except for the animal No. 6 which showed a weight loss of 13% on the day 5 post-dose, but gain the weight of 9 % till end of the study
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- Under the conditions of the present study, a single oral application by gavage of the test item Hexane-1,3,6-tricarbonitrile to rats at a dose of 2000 mg/kg bw was associated with signs of toxicity and mortality.
Under the conditions of the present study, a single oral application by gavage of the test item Hexane-1,3,6-tricarbonitrile to rats at a dose of 300 mg/kg bw was associated with signs of toxicity but no mortality.
The median lethal dose of Hexane-1,3,6-tricarbonitrile after a single oral administration to female rats, observed over a period of 14 days is:
LD50 cut-off (rat): 2000 mg/ kg bw
On the basis of the test results and in conformity with the criteria given in GHS (Globally Harmonized System of Classification and Labelling of Chemicals), the substance should be classified into Category 4: > 300 – 2000 mg/kg bw. - Executive summary:
Under the conditions of the present study, a single oral application by gavage of the test item Hexane-1,3,6-tricarbonitrile to rats at a dose of 2000 mg/kg bw was associated with signs of toxicity and mortality.
Under the conditions of the present study, a single oral application by gavage of the test item Hexane-1,3,6-tricarbonitrile to rats at a dose of 300 mg/kg bw was associated with signs of toxicity but no mortality.
The median lethal dose of Hexane-1,3,6-tricarbonitrile after a single oral administration to female rats, observed over a period of 14 days is:
LD50 cut-off (rat): 2000 mg/ kg bw
On the basis of the test results and in conformity with the criteria given in GHS (Globally Harmonized System of Classification and Labelling of Chemicals) [9], the substance should be classified into Category 4: > 300 – 2000 mg/kg bw.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 2 000 mg/kg bw
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Data waiving:
- exposure considerations
- Justification for data waiving:
- the study does not need to be conducted because exposure of humans via inhalation is not likely taking into account the vapour pressure of the substance and/or the possibility of exposure to aerosols, particles or droplets of an inhalable size
Reference
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2022.06.09-2022.08.24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity: Fixed Dose Procedure)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- fixed dose procedure
- Limit test:
- no
- Species:
- rat
- Strain:
- Wistar
- Remarks:
- WISTAR rats Crl: WI(Han)
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River, 97633 Sulzfeld, Germany
- Age at study initiation: 8-9 weeks
- Weight at study initiation: 193-200g
- Fasting period before study: 24hours
- Housing:Full barrier in an air-conditioned room
- Historical data:
- Diet (e.g. ad libitum): libitum
- Water (e.g. ad libitum):libitum
- Acclimation period:at least 6 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18.7 – 20.9 °C
- Humidity (%): 44 – 63%
- Air changes (per hr): 10 x / hour
- Photoperiod (hrs dark / hrs light): 12 hours light, 12 hours dark - Type of coverage:
- semiocclusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: the dorsal area of the trunk
- % coverage: No less than 10% of the body surface
- Type of wrap if used: a semi-occlusive dressing made of a porous gauze and non-irritating tape and was fixed with an additional dressing in a suitable manner.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): the residual test item was removed using aqua ad injectionem
- Time after start of exposure: 24hours
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2000 mg/kg bw
- Constant volume or concentration used: yes
- Duration of exposure:
- 24 hours
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 3
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: on day 1, days 8 and
- Necropsy of survivors performed: yes - Preliminary study:
- One animal was administered at a dosage of 2000mg/kg bw as the starting dose. As the animal survived, this dose was used as a starting dose for the main study.
- Sex:
- female
- Dose descriptor:
- other: Acute Toxicity Estimate(ATE)
- Effect level:
- > 2 000 - <= 5 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- none
- Body weight:
- lower than 10% body weight loss
- Interpretation of results:
- Category 5 based on GHS criteria
- Conclusions:
- Under the conditions of the present study, single dermal application of the test item Hexane-1,3,6-tricarbonitrile to rats at a dose of 2000 mg/kg bw was associated with no mortality and neither signs of toxicity nor signs of irritation.
According to OECD guideline 402 [4] the test item Hexane-1,3,6-tricarbonitrile has no obligatory labelling requirement for percutaneous toxicity and is not classified.
The Acute Toxicity Estimate according to GHS (Globally Harmonized Classification System), 2021 [9] was determined to be 2000 < ATE ≤ 5000 mg Hexane-1,3,6-tricarbonitrile / kg body weight. - Executive summary:
Under the conditions of the present study, single dermal application of the test item Hexane-1,3,6-tricarbonitrile to rats at a dose of 2000 mg/kg bw was associated with no mortality and neither signs of toxicity nor signs of irritation.
According to OECD guideline 402 [4] the test item Hexane-1,3,6-tricarbonitrile has no obligatory labelling requirement for percutaneous toxicity and is not classified.
The Acute Toxicity Estimate according to GHS (Globally Harmonized Classification System), 2021 [9] was determined to be 2000 < ATE ≤ 5000 mg Hexane-1,3,6-tricarbonitrile / kg body weight.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- > 2 000 mg/kg bw
Additional information
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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