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EC number: 448-170-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experiment start date - 20 April 2003; Experiment completion date - 16 May 2003; Study completion date - 07 July 2003.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- The test method was modified following a method developed by RCC Ltd (Ref. 1) to quantify the algicidal effect of colored test substances, but also the growth inhibition effect caused by reduced light intensities in the colored test media.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- yes
- Remarks:
- The test method was modified following a method developed by RCC Ltd (Ref. 1) to quantify the algicidal effect of colored test substances, but also the growth inhibition effect caused by reduced light intensities in the colored test media.
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Identity: FAT 40810/A
Batch: WP 6/02
Purity: approx. 75 %
Appearance: Solid, dark brownish powder
Expiration date: 12 December 2010
Storage: At room temperature at about 20 °C - Analytical monitoring:
- yes
- Details on sampling:
- For the analysis of the actual test item concentrations duplicate samples without algae were taken from each test concentration and the control just before test start and after 72 hours (end of the test). For the 72-hour stability samples additional flasks with adequate volumes of the freshly prepared test media of all test concentrations and the control were incubated under the same conditions as in the actual test but without algae. All samples were deep-frozen (at about -20 °C) immediately after sampling. Based on pre-experiments for investigation of the storage stability (without GLP) the test item is sufficiently stable in the test water under these storage conditions.
- Vehicle:
- no
- Details on test solutions:
- The test item (29.91 mg) was dissolved in purified water and made up to the mark in a 100 ml volumetric flask to prepare a stock solution of 299 mg/L. Defined volumes of this stock solution were diluted with a mixture of acetonitrile/purified water (70:30; v:v) to obtain standard solutions in the range of 0.897 to 29.9 mg/l of the test item.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- The test organism used for the study was Scenedesmus subspicatus CHODAT, Strain No. 86.81 SAG, supplied by the Collection of Algal Cultures (SAG, Institute for Plant Physiology, University of Gottingen, D-37073 Göttingen, Germany). The algae had been grown in the RCC laboratories under standardized conditions according to the test guidelines.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Hardness:
- 24 mg CaCO3/L
- Test temperature:
- 23 °C throughout the test period
- pH:
- 8.0 - 8.2 at beginning and 8.2 - 9.6 at the end of test
- Nominal and measured concentrations:
- Nominal concentrations : 0 (control), 1.0, 3.2, 10, 32, 100 mg/L
Measured values were in the range of 93 - 110 % of nominal values throughout the test period and thus test item was considered stable during performance of the test. - Details on test conditions:
- The test included two experimental parts:
Experimental part A: The algae grew in test media with dissolved test item in the Erlenmeyer flasks (five test concentrations and a control). All glass dishes above the cylinders contained purified water. Thus, the inhibition of algal growth in this experimental part was caused by a real toxic effect of the test item and in addition to the reduced light intensities in the colored test media in the Erlenmeyer flasks.
Experimental part B: In this experimental part the glass dishes above the cylinders contained the colored test media with the same test concentrations as in part A, however without algae (3 replicates per test concentration). In the Erlenmeyer flasks below, the algae grew in test water without test item (as in the control), however under changed light conditions due to the filter effect of the colored test media in the glass dishes. Thus, the growth inhibition in part B was caused by light absorption only. The depth of the test media in the glass dishes was 4 mm, i.e. half the depth of the test media in the Erlenmeyer flasks, because the algae in the stirred test media stay in the statistical mean in this mean depth. - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 46 mg/L
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- Dunnett-test, one-sided, α = 0.05
- Validity criteria fulfilled:
- yes
- Conclusions:
- ErC50 (scenedesmus subspicatus, 72h): >100 mg/L
- Executive summary:
The influence of the test item FAT 40810/A on the growth of the green algal species Scenedesmus subspicatus was investigated in a 72-hour static test according to the EU Commission Directive 92/69/EEC, Annex Part C.3, 1992, and the OECD Guideline No. 201, 1984. However, the test method was modified to quantify the algicidal effect of the test item, but also the growth inhibition effect caused by reduced light intensities in the colored test solutions. The nominal test concentrations were 1.0, 3.2, 10, 32 and 100 mg/L in parallel with a control. All test media down to the lowest test concentration were slightly to strongly colored by the test item. The analytically determined test item concentrations in the analyzed test media varied in the range from 93 to 110 % of the nominal values at the start and the end of the test. Consequently, the test item was stable during the test period of 72 hours under the test conditions, and the reported biological results are based on the nominal concentration of the test item. The same growth inhibition of Scenedesmus subspicatus was observed when the algae grew in test water without test item, but under reduced light intensities by the filter effect of the colored test media as in the second parallel experimental part, where the algae grew in the test media with dissolved test item. Thus, in conclusion, this modified algal test has clearly demonstrated that the observed growth inhibition effect of the test item FAT 40810/A on Scenedesmus subspicatus was caused only by an indirect effect, the light filter effect in the colored test solutions. Thus, a toxic effect of the test item on the algal cells can be excluded up to the highest test concentration of 100 mg/L.
Reference
Analytical results:
The analytically determined test item concentrations in the analyzed test media varied in the range from 93 to 110 % of the nominal values at the start and the end of the test. Consequently, the test item was stable during the test period of 72 hours under the test conditions, and the reported biological results are based on the nominal concentration of the test item.
Experimental part A: Experimental part A corresponds to the usual algal toxicity test. This means that the algal growth inhibition in this experimental part was caused by a possible toxic effect of the test item and/or by the reduced light intensities due to the light absorption in the colored test media. In experimental part A, the test item had a statistically significant inhibitory effect on the growth rate "r" of Scenedesmus subspicatus after the exposure period of 72 hours first at the concentration of 32 mg/L (results of a Dunnett-test, one-sided, a= 0.05). Thus, this test concentration was determined as the 72-hour LOEC (lowest concentration tested with toxic effects}. The 72-hour NOEC (highest concentration tested without toxic effects after a test period of 72 hours} was determined at the concentration of 10 mg/L, since up to and including this test concentration the mean growth rate r of the algae was statistically not significantly lower than in the control. For the biomass b, the same LOEC- and NOEC-values were determined. The EC values were calculated for the algal biomass b and the growth rate r after 72 hours test duration:
Experimental part A
Parameter (0-72 h) | Blomass b (mg/l) | Growth rate r (mg/l) |
EC50 | 46 | >100 |
EC10 | 4.8 | 20 |
NOEC | 10 | 10 |
LOEC | 32 | 32 |
At the microscopical examination of the shape of the algal cells after 72 hours incubation period no difference was observed between the algae growing in the test item concentration of 100 mg/L in experimental part A and the algal cells in the control. Thus, the shape and size of the algal cells, growing at this concentration of dissolved test item were obviously not affected.
Experimental part B: In experimental part B the algal growth inhibition caused by the pure light effect (the reduced light intensities in the colored test media) was quantified. In this experimental part a nearly identical inhibition effect on the algal growth was observed compared to experimental part A. The EC values in experimental part B were in the same magnitude as the corresponding EC values in experimental part A. The EC values in experimental part 8 are listed below:
Parameter (0-72 h) | Biomass b (mg/L) | Growth rate r (mg/L) |
EC50 | 44 | >100 |
|
|
|
EC10 | 2.8 | 17 |
|
|
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Comparison between the results in experimental parts A and B
According to the recommendations of the Ad-hoc working group of experts on algal growth inhibition for the interpretation of test results of colored substances the comparison between the results in experimental parts A and 8 was based on the growth rates. The differences between the results of experimental parts A and B were described for each test concentration as percentage inhibition of the growth rate rA (lrA) minus the percentage inhibition of the growth rate rB (Ire) after the 72 hours test period. At all test concentrations these differences were lower than 10 %. As another measure of difference, the quotient of the growth rates rA/rB was calculated for each test concentration. At all test concentrations this quotient was at least 0.9 or higher. Differences in growth rates up to the magnitude of 10 % are accepted to be caused by pure chance in the used algal toxicity test. Thus, according to the recommendations of the Ad-hoc working group of experts on algal growth inhibition tests for colored substances the differences between inhibition in experimental part A and B should be not higher than 10 %, and the quotient rA/rB should be at least 0.9 or higher to accept that the inhibition curves of the growth rates rA and rB are essentially the same. At all test concentrations of this test, the differences of the inhibition of growth rates rA and rB are lower than 10 % and the quotients rA/rB are at least 0.9.
Description of key information
The NOEC and ErC50 towards scenedesmus subspicatus was set to >100 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
The influence of the test item FAT 40810/A on the growth of the green algal species Scenedesmus subspicatus was investigated in a 72-hour static test according to the EU Commission Directive 92/69/EEC, Annex Part C.3, 1992, and the OECD Guideline No. 201, 1984. However, the test method was modified to quantify the algicidal effect of the test item, but also the growth inhibition effect caused by reduced light intensities in the colored test solutions. The nominal test concentrations were 1.0, 3.2, 10, 32 and 100 mg/L in parallel with a control. All test media down to the lowest test concentration were slightly to strongly colored by the test item. The analytically determined test item concentrations in the analyzed test media varied in the range from 93 to 110 % of the nominal values at the start and the end of the test. Consequently, the test item was stable during the test period of 72 hours under the test conditions, and the reported biological results are based on the nominal concentration of the test item. The same growth inhibition of Scenedesmus subspicatus was observed when the algae grew in test water without test item, but under reduced light intensities by the filter effect of the colored test media as in the second parallel experimental part, where the algae grew in the test media with dissolved test item. Thus, in conclusion, this modified algal test has clearly demonstrated that the observed growth inhibition effect of the test item FAT 40810/A on Scenedesmus subspicatus was caused only by an indirect effect, the light filter effect in the colored test solutions. Thus, a toxic effect of the test item on the algal cells can be excluded up to the highest test concentration of 100 mg/L.
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