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EC number: 404-170-0 | CAS number: 70750-63-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study; well documented study report
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Details on test material:
- - Physical state: Blue powder
- Analytical purity: 96.3%
- Composition of test material, percentage of components: NDA
- Storage condition of test material: At room temperature in the dark in the container in which it was received.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- -Concentrations: the concentration of the test material in the test solutions were impossible to be verified by chemical analysis due to its low solubility in organic solvents other than dimethylformamide (DMF).
-Sampling method: at the start of the test, a sample of the stock was taken and analyzed for the concentration of the test substance; at the end of the test each blank solution was sampled and analyzed in the same manner.
- Sample storage conditions before analysis: No data.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Nominal exposure concentration preparations.
- Controls: Blank control and solvent control.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Dimethylformamide (DMF)
- Concentration of vehicle in test medium (stock solution and final test solution): 0.1 ml DMF/L in culture medium.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): The test solutions were clear by visual assessment
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Selenastrum capricornutum
- Strain: ATCC 22662
- Source (laboratory, culture collection): From laboratory cultures maintained under axenic conditions.
- Age of inoculum (at test initiation): A culture of the alga in the exponential growth phase was used as inoculum of the test.
- Method of cultivation: No data.
ACCLIMATION
- Acclimation period: No data.
- Culturing media and conditions (same as test or not): Yes.
- Any deformed or abnormal cells observed: No data.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- No data
- Test temperature:
- The daily temperature measurements recorded in the incubator ranged from 23.3 to 23.9 °C. The hourly temperature measurements recorded automatically, ranged from 24.4 to 25.0 °C.
- pH:
- At the start of the test, the pH of all the test solutions was 7.4, and at the end of the test the range was 7.9-8.1.
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal concentrations: 0.010, 0.018, 0.032, 0.056, 0.1 mg/L, with a control and solvent control
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: The test vessels were borosilicate glass conical flasks of 250 ml nominal capacity closed with polyurethane foam bungs. Each algal test falsk contained 100 ml of test sulutions.
- Initial cells density: 1.03 x 104 cells /ml.
- Control end cells density: 1.03 x 104 cells /ml.
- No. of organisms per vessel: 1.03 x 106 cells in 100 ml media.
- No. of vessels per concentration (replicates): triplicate each concentration of the test substance.
- No. of vessels per control (replicates): triplicate cultures of the culture medium control
- No. of vessels per vehicle control (replicates): 6 replicate cultures of the solvent control
- Other: One blank vessel (without algal inoculum) for each control and treatment was incubated concurrently.
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: Yes.
- Light intensity and quality: measured once during the study, was 8420 lux (by cosine receptor). This was also measured in terms of quantum response and was 66.4 microeinsteins/m2/sec
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter.
TEST CONCENTRATIONS
No range finding study was performed. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- exceeding solubility
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- exceeding solubility
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks:
- exceeding solubility
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks:
- exceeding solubility
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- No data
Any other information on results incl. tables
Table 1. Algal Cell Densities
Nominal Conc. of Test Material ( mg/L) |
Algal cell density (x 104cell/ml) (Mean) |
||
Day 1 |
Day 2 |
Day 3 |
|
Control (N = 3) |
6.07 |
38.2 |
211 |
Solvent Control (N = 6) |
6.06 |
38.9 |
201 |
0.01 |
5.44 |
38.2 |
204 |
0.018 |
5.12 |
33.1 |
193 |
0.032 |
5.36 |
38.4 |
207 |
0.056 |
5.58 |
35.6 |
201 |
0.10 |
5.34 |
34.2 |
184 |
Table 2. Mean Areas Under The Growth Curve, And Percentages Of The Solvent Control Indicating Statistically Significant Differences
Nominal Conc. of Test Material ( mg/L) |
Mean Area under Growth Curve (0-72 h) |
% of Solvent Control |
Control |
147.1 |
103 |
Solvent Control |
143.1 |
- |
0.010 |
142.9 |
100 |
0.018 |
132.1 |
92 |
0.032 |
144.7 |
101 |
0.056 |
139.1 |
97 |
0.10 |
128.9 |
90 |
There were no statistically significant differences from the solvent control (P = 0.05).
Table 3. Man Growth Rates Over The Test Period, And Percentages Of The Solvent Control Indicating Statistically Significant Differences
Nominal Conc. of Test Material ( mg/L) |
Mean Growth Rate (0-72 h) |
% of Solvent Control |
Control |
1.774 |
101 |
Solvent Control |
1.758 |
- |
0.010 |
1.726 |
100 |
0.018 |
1.744 |
99 |
0.032 |
1.768 |
101 |
0.056 |
1.758 |
100 |
0.10 |
1.728 |
98 |
There were no statistically significant differences from the solvent control (P = 0.05).
Table 4. Test Solution pH Measurements
Nominal Conc. of Test Material ( mg/L) |
Day 0 |
Day 3 |
|
Replicate A |
Replicate B |
||
Control |
7.4 |
8.0 |
8.1 |
Solvent Control |
7.4 |
8.1 |
8.0 |
0.010 |
7.4 |
8.0 |
8.0 |
0.018 |
7.4 |
7.9 |
8.0 |
0.032 |
7.4 |
8.0 |
8.1 |
0.056 |
7.4 |
7.9 |
8.1 |
0.10 |
7.4 |
7.9 |
8.0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC values based on the 72 h nominal test concentration were greater than 0.1 mg/L, correspondingly the NOEC was greater than or equal to 0.1 mg/L based on areas under the growth curve, or on logarithmic growth rate over the test period.
- Executive summary:
In a 72 h acute toxicity study, the cultures of the green algaSelenastratum capricornutumwere exposed to the test material at a nominal concentrations of 0.010, 0.018, 0.032, 0.056, 0.1 mg/L, with a control and solvent control, under static conditions in accordance with OECD 201. The NOEC and EC50values based on logarithmic growth rate over the test period were 0.10 and >0.10 mg/L (nominal), respectively. The NOEC and EC50values based on areas under the growth curve were 0.10 and >0.10 mg/L (nominal), respectively. There were no compound related phytotoxic effects.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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