Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 953-704-3 | CAS number: 2411191-47-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2020-11-13 to 2020-11-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Study performed according to OECD test guideline No. 492 and in compliance with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 492 (Reconstructed Human Cornea-like Epithelium (RhCE) Test Method for Identifying Chemicals Not Requiring Classification and Labelling for Eye Irritation or Serious Eye Damage)
- Version / remarks:
- 18 June 2019
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (4E)-4-(3,3,4S-trimethylcyclopentylidene)butanal
- Molecular formula:
- C12 H20 O
- IUPAC Name:
- (4E)-4-(3,3,4S-trimethylcyclopentylidene)butanal
- Reference substance name:
- (4E)-4-(3,3,4R-trimethylcyclopentylidene)butanal
- Molecular formula:
- C12 H20 O
- IUPAC Name:
- (4E)-4-(3,3,4R-trimethylcyclopentylidene)butanal
- Test material form:
- liquid
- Details on test material:
- Appearance: Very pale yellow liquid
Constituent 1
Constituent 2
Test animals / tissue source
- Species:
- human
- Strain:
- other: Human Keratinocyte
- Details on test animals or tissues and environmental conditions:
- - Justification of the test method and considerations regarding applicability: The EpiOcular™ EIT was validated by the European Union Reference laboratory for Alternatives to Animal Testing (EURL ECVAM) and Cosmetics Europe between 2008 and 2013. The EpiOcular™ EIT was endorsed as an in vitro test that can be used to identify those chemicals not requiring classification and labelling for eye irritation or serious eye damage in accordance with UN GHS (No Category).
- Description of the cell system used, incl. certificate of authenticity and the mycoplasma status of the cell live: EpiOcularTM Human Corneal Model (0.6 cm2 ) provided by MatTek In Vitro Life Science Laboratories, Bratislava – Slovakia. No biological contaminant detected.
- Cell line used, its source: Keratinocyte strain 4F1188.
- RhCE tissue used, including batch number: EpiOcular Tissue Lot Number: 30634.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent positive control
- yes, concurrent negative control
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 µL - Duration of treatment / exposure:
- 30 ±2 minutes at 37 °C, 5% CO2
- Duration of post- treatment incubation (in vitro):
- 120 ±15 minutes at 37 °C, 5% CO2
- Number of animals or in vitro replicates:
- Duplicate cultures for test item, negative and positive controls
- Details on study design:
- - Details of the test procedure used: This method is based on the MatTek Corporation Protocol: EpiOcular™ Eye Irritation Test (OCL-200-EIT) for the prediction of acute ocular irritation of chemicals; for use with MatTek Corporation’s Reconstructed Human EpiOcular™ Model; 02 October 2017.
- Volume of test chemical and control substances used: 50 µL
- Duration and temperature of exposure period: 30 ±2 minutes at 37 °C, 5% CO2
- Duration and temperature of post-exposure immersion period: 12 ±2 minutes at room temperature
- Duration and temperature of post-exposure incubation period: 120 ±15 minutes at 37 °C, 5% CO2
- Justification for the use of a different negative control than ultrapure H2O (if applicable): not applicable
- Justification for the use of a different positive control than neat methyl acetate (if applicable): not applicable
- Description of any modifications to the test procedure: none
- Indication of controls used for direct MTT-reducers and/or colouring test chemicals (if applicable): not applicable
- Number of tissue replicates used per test chemical and controls (positive control, negative control, NSMTT, NSCliving and NSCkilled, if applicable): not applicable
- Description of the method used to quantify MTT formazan, if applicable: The optical density (OD) was measured at 570 nm (OD570) using a Labtech LT-4500 microplate reader. The upper limit of accuracy for measured absorbance of MTT Formazan at 570 nm (OD570), filter band pass 10 nm, was determined to be at an optical density of 2.6.
- Description of evaluation criteria used including the justification for the selection of the cut-off point for the prediction model: The relative mean tissue viabilities were compared to the mean of the negative control (n=2) treated tissues. The relative mean tissue viabilities were calculated as follows: Relative mean tissue viability = mean OD570 of test item mean / OD570 of negative control x 100. Mean Tissue Viability >60% : No Category / Mean Tissue Viability ≤60% : No prediction can be made according to the EU CLP and the GHS.
- Reference to historical positive and negative control results demonstrating suitable run acceptance criteria: In addition to the acceptability criteria a comparison of laboratory historical data on negative and positive controls was made to verify the functioning of the test system.
- Complete supporting information for the specific RhCE tissue construct used: yes, Certificate of Analysis from MatTek is attached to the report.
- Reference to historical data of the RhCE tissue construct: Acceptance criteria were met for Tissue Viability, Barrier function and Sterility.
- Demonstration of proficiency in performing the test method before routine use by testing of the proficiency chemicals: not included in the report, but available upon request to the lab
Results and discussion
In vitro
Results
- Irritation parameter:
- mean percent tissue viability
- Run / experiment:
- Mean of duplicate cultures
- Value:
- 73.2
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks:
- 36.7%
- Remarks on result:
- no indication of irritation
- Other effects / acceptance of results:
- OTHER EFFECTS:
- Visible damage on test system: none
ACCEPTANCE OF RESULTS:
- Positive and negative control means and acceptance ranges based on historical data: All values for the control groups were within the ranges obtained by the testing laboratory.
- Acceptable variability between tissue replicates for positive and negative controls:
The relative mean tissue viability for the positive control treated tissues was 36.7% relative to the negative control treated tissues (below 50%). The positive control acceptance criterion was therefore satisfied.
The mean OD570 for the negative control treated tissues was 2.104 (> 0.8 and < 2.8). The negative control acceptance criterion was therefore satisfied.
- Acceptable variability between tissue replicates for the test chemical: The difference of viability between the two relating tissues of a single test item is < 20% in the same run (for positive and negative control tissues and tissues of test items). This applies also to the killed controls (test items and negative killed control) and the colorant controls which are calculated as percent values related to the viability of the relating negative control.
Any other information on results incl. tables
Table 7.3.2/1: Individual Scores and Mean Scores for Corneal Effects – Test Item
Item | OD570 of tissues (tvt) | OD570 Tissues corrected for[tkt-ukt] | Correct Mean OD570 of duplicate tissues | Individual tissue viability (%) | Relative mean viability (%) | Difference in viability (%) |
Negative Control Item | 2.209 | - | 2.104 | 105.0 | 100* | 10.0 |
1.999 | - | 95.0 | ||||
Positive Control Item | 0.737 | - | 0.771 | 35.0 | 36.7 | 3.3 |
0.805 | - | 38.3 | ||||
Test Item | 1.540 | 1.498 | 1.539 | 71.2 | 73.2 | 3.9 |
1.622 | 1.580 | 75.1 |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Under the test conditions, the test item was classified as non-irritant according to the Regulation (EC) No. 1272/2008 (CLP) and to the GHS.
- Executive summary:
The purpose of this study was to identify chemicals not requiring classification and labelling for eye irritation or serious eye damage using the EpiOcular™ Eye Irritation Test (EIT) according to the OECD Test Guideline 492 Reconstructed human Cornea-like Epithelium (RhCE) test method.
Duplicate tissues were treated with the test item for an exposure period of 30 minutes. At the end of the exposure period each tissue was rinsed before incubating for 120minutes. The test item was found to directly reduce MTT and therefore additional non-viable, freeze-killed, tissues were incorporated into the testing for correction purposes. At the end of the post-exposure incubation period each tissue was taken for MTT-loading. After MTT-loading each tissue was placed in 2 mL of isopropanol for MTT extraction. At the end of the formazan extraction period each well was mixed thoroughly and duplicate 200 µL samples were transferred to the appropriate wells of a pre-labeled 96-well plate. The optical density (OD) was measured at 570 nm (OD570). Data are presented in the form of percentage viability (MTT reduction in the test item treated tissues relative to negative control tissues).
The relative mean viability of the test item treated tissues, corrected for direct MTT reduction, was 73.2%.
The criteria required for acceptance of results in the test were satisfied.Under the test conditions, the test item was classified as non-irritant according to the Regulation (EC) No. 1272/2008 (CLP) and to the GHS.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.