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EC number: 458-610-1 | CAS number: 60466-73-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From January 16, 2012 to January 20, 2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- This study was performed according to national and international guidelines and is considered reliable with restrictions. No dose-response curve was observed. The LC50 value was determined as a geometric mean of LC0 and LC100. Test fish was a bit bigger than recommended : 2,6 +/- 1,7 cm (recommended 2 +/- 1 cm).The Certificate of Analysis of the test substance was not provided in the study report. Moreover, the substance is adequately identified, but details on composition are missing.
- Qualifier:
- according to guideline
- Guideline:
- other: 203 Fish Acute toxicity test. China Environmental Science Press
- Version / remarks:
- State Environmental Protection Administration of China. The Guidelines for the Testing of Chemicals, 203 Fish Acute toxicity test. China Environmental Science Press. 2004.
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- OECD. OECD Guidelines for the Testing of Chemicals, 203 Fish Acute Toxicity Test. Paris: OECD, Adopted 17 July 1992.
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: The sample of blank control and 6.87mg/L, 11.0mg/L, 17.6mg/L test groups were directly analyzed after it were filtered through 0.45μm membrane. And the test solutions of 28.1mg/L and 45.0mg/L test groups were filtered by 0.45μm filter membrane, the filtrate diluted 2 times were analyzed. These samples were analyzed immediately. Otherwise the samples were stored at 4°C until analysis.
- Vehicle:
- no
- Details on test solutions:
- Preparation of test stock solution : 0.500g test substance was dissolved in some test water with ultrasonic treatment for 10min. Then the solution was transferred into 5L-jar, diluted with the test water to 5000mL and was stirred with a magnetic stirrer for 20min. Filtered with 0.45μm filter membrane. Abandoned the suspended fraction, the saturation concentration of filtered test solution of nominal concentration of 100mg/L test substance solution was obtained. The saturation solution was prepared every day (Three copies of the saturation solution were prepared at 0h after start of the test; one saturation solution was respectively prepared at 24h, 48h and 72h after start of the test).
The actual concentration of the saturation solution prepared every day was measured, the data of the actual concentrations were 85.42mg/L at 0h after start of the test, 84.82mg/L at 24h after start of the test, 79.55mg/L at 48h after start of the test and 73.72mg/L at 72h after start of the test. Then it was used to prepare the test solutions of each exposure level of test substance.
- Test concentrations : Five test concentrations of 6.87 mg/L, 11.0 mg/L, 17.6 mg/L, 28.1 mg/L and 45.0 mg/L as measured concentration of stock solution with a geometric series of a factor 1.6 (6.040 mg/L, 9.769 mg/L, 15.78 mg/L, 25.56 mg/L and 40.37mg/L as measured concentration in geometric mean). One blank control containing no test substance was set to the test series, too.
- The dissolved oxygen concentration in the test solutions was kept at least 60 percent of the air saturation value throughout the test. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Source : Guangzhou Yang Shi Breeding Farm
- Common name: Danio rerio (previous name: Brachydanio rerio)
- Length at study initiation (length definition, mean, range and SD): 10 fish were randomly selected and measured on January 11, 2012. The average and standard deviation of the population from which the fish were selected were 26.04 mm ± 1.71 mm at total length
- Weight at study initiation (mean and range, SD): the average and standard deviation were 0.186 g ± 0.053 g at weight.
ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): under the same conditions of water quality (Dechlorinated tap water), temperature (23 ± 2 °C), light and dark period (14h light / 10h dark). The dissolved oxygen concentration during acclimation was kept at least 80 % of the air saturation value. The test fish were fed daily until 24 hours before the start of the test.
- Feeding frequency during acclimation: The test fish were fed daily until 24 hours before the start of the test.
- Health during acclimation (any mortality observed): The mortality was 0% during 7 days before the start of the test, which met the requirement.
FEEDING DURING TEST
- No feeding during the test - Test type:
- semi-static
- Water media type:
- other: dechlorinated tap water
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 141 mg/L (expressed as CaCO3)
- Test temperature:
- 23 +/- 2°C
- pH:
- pH range : 7.96 - 8.43
- Dissolved oxygen:
- 60 to 84 %
- Salinity:
- Not applicable
- Conductivity:
- Not available
- Nominal and measured concentrations:
- Nominal concentrations: 6,87 mg/l, 11,0 mg/L, 17,6 mg/L, 28,1 mg/L and 45,0 mg/L
Measured concentrations (geometric means): 6.040 mg/L, 9.769 mg/L, 15.78 mg/L, 25.56 mg/L and 40.37 mg/L - Details on test conditions:
- TEST SYSTEM
- Type (delete if not applicable): open test vessels
- Material, size, headspace, fill volume: 3L / test vessel
- Aeration: no aeration
- Renewal rate of test solution (frequency/flow rate): every 24h
- No. of organisms per vessel: 7 fish / test vessel
- No. of vessels per concentration (replicates): 3 replicates per test group
- No. of vessels per control (replicates): 1 blank control
OTHER TEST CONDITIONS
- Photoperiod: 14h light / 10h darkness
- Light type: Artificial light of white fluorescent lamp
- The dissolved oxygen concentration in the test solutions was kept at least 60 percent of the air saturation value throughout the test.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality and visible abnormality of the test fish were recorded at 3h, 24h, 48h, 72h and 96h after the start of the test during test period. The temperature, pH and dissolved oxygen of each test group were measured at 0h, 96h and 24h, 48h, 72h (before and after the renewal) after the start of the test. The actual concentrations of test substance of blank control and setting concentration 6.87 mg/L, 11.0 mg/L, 17.6 mg/L, 8.1 mg/L and 45.0 mg/L test groups were measured at 0h, 96h and 24h, 48h, 72h (before and after the renewal) by a High Performance Liquid Chromatography (HPLC).
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.6
- Test concentrations: Five test concentrations : 6,87 mg/l, 11,0 mg/L, 17,6 mg/L, 28,1 mg/L and 45,0 mg/L (measured concentration of stock solution, factor 1,6). - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate was used as reference substance.
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 20.1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL not available
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 15.78 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 25.56 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: The mortalities of the controls were less than 10% at the end of the test.
- Abnormal responses: No abnormal response was observed in blank control. Observed abnormal responses during the test were reduced activity, overturn and loss of equilibrium and immobilized at the bottom; lying on the bottom; at the surface; swimming at the bottom; spasmodic swimming.
Observed abnormal responses are shown in Table 2. - Results with reference substance (positive control):
- The test result of Zebra fish (Brachydanio rerio) sensitivity test revealed that 24h-LC50 of the reference substance K2Cr2O7 was 307 mg/L with 95% confidence limits (L95) of 273 mg/L - 346 mg/L, within the range of 200 mg/L-400 mg/L. See Overall remarks and attachments.
- Sublethal observations / clinical signs:
Concentration of test substance in test solution:
The actual concentration of test substance in blank control were all <0.01647mg/L (LOD) at 0h, 96h and 24h, 48h, 72h (before and after the renewal) after the start of the test. Analysis of the test solutions with concentration of 6.87 mg/L, 11.0 mg/L, 17.6 mg/L, 28.1 mg/L and 45.0 mg/L at 0h, 96h and 24h, 48h, 72h (before and after the renewal) after the start of the test. The measured concentrations of test substance in the test solution at the start of the exposure and after the renewal ranged from 5.898 mg/L to 42.72 mg/L (86% to 95% of the setting concentration of test substance). That before the renewal and at the end of the exposure ranged from 5.484 mg/L to 38.14 mg/L (80% to 85% of the setting concentration of test substance). During the test, the actual concentration of each exposure level can be maintained within 80%-120% of the setting concentration of test substance.
Table 1 : Cumulative mortality (%)
Settingconcentration
(mg/L)
Number of test fish
Cumulative mortality(%)
3h
24h
48h
72h
96h
Blank control
21
0
0
0
0
0
6.87
21
0
0
0
0
0
11.0
21
0
0
0
0
0
17.6
21
0
0
0
0
0
28.1
21
48
100
100
100
100
45.0
21
100
100
100
100
100
Table 2 : Observed abnormal response
Settingconcentration
(mg/L)
Rep-
licate
Result of observation
(Left column: Number of affected fish / Total survival number, Right column: Symptom detail)
3h
24h
48h
72h
96h
Blank control
1
0/7
N
0/7
N
0/7
N
0/7
N
0/7
N
2
0/7
N
0/7
N
0/7
N
0/7
N
0/7
N
3
0/7
N
0/7
N
0/7
N
0/7
N
0/7
N
6.87
1
0/7
N
0/7
N
0/7
N
0/7
N
0/7
N
2
0/7
N
0/7
N
0/7
N
0/7
N
0/7
N
3
0/7
N
0/7
N
0/7
N
0/7
N
0/7
N
11.0
1
0/7
N
0/7
N
0/7
N
7/7
IMM(4)
RA(3)
7/7
IMM(2)
RA(5)
2
0/7
N
0/7
N
0/7
N
7/7
IMM(7)
7/7
IMM(6)
RA(1)
3
0/7
N
0/7
N
0/6
N
7/7
IMM(3)
RA(4)
7/7
IMM(2)
RA(5)
17.6
1
7/7
IMM(1)
RA(6)
7/7
IMM(7)
7/7
IMM(5)
OLE(2)
7/7
IMM(5)
OLE(2)
7/7
IMM(6)
OLE(1)
2
7/7
IMM(2)
RA(5)
7/7
IMM(7)
7/7
IMM(7)
7/7
IMM(7)
7/7
IMM (7)
3
7/7
OLE(1)
IMM(2)
RA(4)
7/7
IMM(7)
7/7
IMM(6)
OLE(1)
7/7
IMM(5)
OLE(2)
7/7
IMM(5)
OLE(2)
28.1
1
5/5
LB(5)
—
—
—
—
—
—
—
—
2
2/2
LB(2)
—
—
—
—
—
—
—
—
3
4/4
SS(2)
LB(2)
—
—
—
—
—
—
—
—
45.0
1
—
—
—
—
—
—
—
—
—
—
2
—
—
—
—
—
—
—
—
—
—
3
—
—
—
—
—
—
—
—
—
—
Note: “N”— Normal; “—”: All test fish died;“RA”: Reduced activity; “OLE”: overturn andloss of equilibrium;“IMM”:Immobilized at the bottom; “LB”:lying on the bottom; “AS”:at the surface;“AB”swimming at the bottom;“SS”:spasmodic swimming.
- Validity criteria fulfilled:
- yes
- Remarks:
- 0% mortality in the blank control at the end of the test and dissolved oxygen concentrations were kept at least 60 percent of the air saturation value.
- Conclusions:
- Under the conditions of the test, the 96h-LC50 of test substance (based on the actual concentration) was 20.1 mg/L for Zebra fish (Brachydanio rerio) and the 95% confidence limit is not available. As no dose-response curve was observed, the LC50 value was determined as a geometric mean of LC0 and LC100.
The highest concentration causing 0% mortality at 96h (LC0) was 15.78 mg/L (based on geometric mean measured concentration of 17.6 mg/L test group).
The lowest concentration causing 100% mortality at 96h (LC100) was 25.56 mg/L (based on geometric mean measured concentration of 28.1 mg/L test group). - Executive summary:
The acute toxicity of the test substance to Danio rerio was assessed in a study following three guidelines : (1) State Environmental Protection Administration of China, The Guidelines for the Testing of Chemicals, 203 Fish Acute toxicity test, China Environmental Science Press, 2004 ; (2) OECD Guidelines for the Testing of Chemicals, 203 Fish Acute Toxicity Test. Paris: OECD, Adopted 17 July 1992 and (3) State Environmental Protection Administration of China, The Guidelines for the test of chemical (HJ/T 153-2004), Beijing, China Environmental Science Press, 2004.
The following five concentrations were tested : 6,87 mg/l, 11,0 mg/L, 17,6 mg/L, 28,1 mg/L and 45,0 mg/L (measured concentration of stock solution, factor 1.6).
10 fish were randomly selected and measured (at study initiation), the average and standard deviation of the population from which the fish were selected were 26.04 mm ± 1.71 mm total length. They were also weighed, the average and standard deviation were 0.186 g ± 0.053 g.
The test was performed at 23 +/- 2°C, with 7 fish / test vessel and 3 replicates were used per test group.
The recordred 96h-LC50 is 20.1 mg/L, based on measured concentrations.
This study is considered reliable with restrictions. No dose-response curve was observed. The LC50 value was determined as a geometric mean of LC0 and LC100. In addition, test fish was a bit bigger than recommended : 2,6 +/- 1,7 cm (recommended 2 +/- 1 cm)
Reference
Description of key information
Chinese 203 Fish Acute toxicity test (2004) and OECD 203 Fish Acute Toxicity Test (1992), Guangdong detection center of microbiology, GLP, 2012:
96h-LC50 (Danio rerio) = 20.1 mg/L (based on measured concentrations)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 20.1 mg/L
Additional information
The acute toxicity of the test substance to Danio rerio was assessed in a study following three guidelines : (1) State Environmental Protection Administration of China, The Guidelines for the Testing of Chemicals, 203 Fish Acute toxicity test, China Environmental Science Press, 2004 ; (2) OECD Guidelines for the Testing of Chemicals, 203 Fish Acute Toxicity Test. Paris: OECD, Adopted 17 July 1992 and (3) State Environmental Protection Administration of China, The Guidelines for the test of chemical (HJ/T 153-2004), Beijing, China Environmental Science Press, 2004. The test was performed according to GLP and validity criteria were fulfilled. However, this study was considered reliable with restrictions as no dose-response curve was observed. The LC50 value was determined as a geometric mean of LC0 and LC100. In addition, test fish was a bit bigger than recommended : 2,6 +/- 1,7 cm (recommended 2 +/- 1 cm).
The following five concentrations were tested : 6,87 mg/l, 11,0 mg/L, 17,6 mg/L, 28,1 mg/L and 45,0 mg/L (measured concentration of stock solution, factor 1.6).
10 fish were randomly selected and measured (at study initiation), the average and standard deviation of the population from which the fish were selected were 26.04 mm ± 1.71 mm total length. They were also weighed, the average and standard deviation were 0.186 g ± 0.053 g. The test was performed at 23 +/- 2°C, with 7 fish / test vessel and 3 replicates were used per test group.
The recorded 96h-LC50 is 20.1 mg/L, based on measured concentrations.
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