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EC number: 609-099-0 | CAS number: 352303-67-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 Aug 2018- 24 August 2018
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: MEP, P.R. China, the Guidelines for the Testing of Chemicals, Effects on Biotic Systems (the Second Edition), No. 209 "Activated Sludge, Respiration Inhibition Test". (2013.8).
- Version / remarks:
- Second Edition
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (2-fluoro-3-methoxyphenyl)boronic acid
- EC Number:
- 609-099-0
- Cas Number:
- 352303-67-4
- Molecular formula:
- FC6H3(OCH3)B(OH)2
- IUPAC Name:
- (2-fluoro-3-methoxyphenyl)boronic acid
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- Lot number: 18051400
Appearance: Off-white powder
Purity: 100.2%
Storage: Room temperature. 15-25°C. Container closed when not in use.
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The activated sludge was derived from a treatment plant receiving predominantly domestic sewage. A fresh sample of activated sludge was collected from the aeration tank of Shenyang North Sewage Treatment Plant 1 day before the test was started.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- DOC for total respiration during 10 minutes measurement period after three hours.
Test conditions
- Test temperature:
- 19.8 - 21.3 C
- pH:
- 6.55- 7.33
- Dissolved oxygen:
- After 3 hrs, the concentration of dissolved oxygen was continuously measured and recorded for 10 minutes.
- Nominal and measured concentrations:
- In the preliminary test, the test concentrations included 10, 100, 300, 1000 mg/L. According to the result of the preliminary test, significant inhibition of oxygen consumption by the test substance was observed at the concentrations of 10- 1000 mg/L. Therefore, the definitive test was carried out at 6 nominal concentrations of 1, 4, 16, 63, 251 and 1000 mg/L with 5 replicates.
- Details on test conditions:
- The inoculums blank controls (FBT), reference item mixtures (FRT) and test item mixtures (FTT) were prepared. Each incubation was started in tum at intervals of about 15 minutes. The exposure was considered to begin with the initial contact of the activated sludge inoculum with the other constituents of the final mixture.
6 DO probes were used for the measurement of the dissolved oxygen, which facilitated multiple simultaneous measurements. Inoculum was added at the same time to 6 test vessels at each time interval.
After the exposure period of 3 hours, a sample from the first aeration vessel was transferred to the measuring cell, an oxygen probe with a sleeve adaptor was then inserted into the neck of the flask, and the magnetic stirrer was started, while the concentration of dissolved oxygen was immediately measured. The concentration of dissolved oxygen was continuously measured and recorded for 10 minutes. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (3,5-DCP) was used as a reference item
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 47.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 3.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- Results with the reference substance: 3, 5 dichlorophenol EC50 (3 hours) in the definite test indicated 4.6 mg/l.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The validity criteria have been satisfied.
- Conclusions:
- Moderate concern for toxicity of micro organisms due to the reported EC50 (3 hrs) was found to be 47.9 mg/L (> 10mg/L (high concern) and < 100 mg/L (low concern))
- Executive summary:
The study was conducted to determine the inhibition effect of FMPBA on micro-organisms from activated sludge (largely bacteria) by measuring their total respiration rate. The contact time for exposure was 3 hours.
In the preliminary test, the test concentrations included 10, 100, 300, 1000 mg/L. The 10, 100, 300 mg/L concentrations were tested with 1 replicate, 1000 mg/L was tested with triplicate and an abiotic control at concentration of 1000 mg/L was performed.
According to the result of the preliminary test, significant inhibition of oxygen consumption by the test substance was observed at the concentrations of 10- 1000 mg/L. Therefore, the definitive test was carried out at 6 concentrations of 1, 4, 16, 63, 251 and 1000 mg/L with 5 replicates. Inoculums blank controls were performed with 6 replicates and prepared at the beginning, middle and end of the exposure period.
Results with the reference substance: 3, 5 dichlorophenol EC50 (3 hours) in the definite test indicated 4.6 mg/l.
Results with the test substance: FMPBA EC10 (3 hours) in the definite test indicated 3.9 mg/l and a EC50 of 47.9 mg/l.
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