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EC number: 619-269-6 | CAS number: 97398-80-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- Sep 08, 2010 - Oct 16, 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):activated sludge, micro organisms from a domestic waste water treatment plant- OriginMunicipal sewage treatment plant.
- Conditioning:The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in tap water and centrifuged again. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- According to guideline
- Reference substance:
- aniline
- Preliminary study:
- The biodegradation in % was calculated based on the ThOD of the test item.
- Test performance:
- The reference item (Aniline) was sufficiently degraded to 77 % after 14 days and to 78 % after 28 days of incubation.The validity criterion that the degradation should be > 60 % after 14 d was fulfilled.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Results with reference substance:
- Comments: the degradation of the test item did not reach 60 % within the 10-day window and after 28 days of incubation. Therefore, the test item is not considered to be readily biodegradable.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
- Executive summary:
Purpose
The test item was investigated for its ready biodegradability in a Manometric Respirometry Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the micro organisms during exposure. As a reference item aniline was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. The GLP study was performed according to OECD TG 301C.
Result
Biodegradation of test item
After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28 -day exposure period a degradation of 0% as found.
Biodegradation of aniline
The reference item was sufficiently degraded to 77 % after 14 days and to 78 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.
Conclusion
The degradation rate of the test material did not reach 60 % within the 10 -day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Dec 12, 2006 - Feb 26, 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Source / origin: Activated sludge, microorganisms from a domestic waste water treatment plant was supplied by the sewage plant Rossdorf, Germany.
Conditioning: The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in tap water and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 1.5 g dry material/L were mixed with test water and then aerated until use. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 103 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Reconstituted Test Water according to OECD 301
- Additional substrate: no
- Test temperature: 22 °C
- pH: 7.6 (measured at the start of the test)
7.7 - 7.9 (measured at the end of the test)
- pH adjusted: no
- Aeration of dilution water: yes
- Continuous darkness: yes
- surrounding: Climate chamber
TEST SYSTEM
- Culturing apparatus: Manometric Test System with test flasks containing a volume of 500 mL
- Number of culture flasks/concentration: 2
- Measuring equipment: BSB/BOD-Sensor-System, Aqualytic, 63231 Neu Isenburg, Germany
- Test performed in closed vessels due to significant volatility of test substance: yes
CONTROL AND BLANK SYSTEM
- Inoculum blank: inoculum and test medium
- Procedure control: reference item, inoculum and test medium
- Abiotic sterile control: test item and test medium
- Toxicity control: test item, reference item, inoculum and test medium - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Under the test conditions, the degradation rate of the test item did not reach 60 % within the 10-day window and after 28 days of incubation. In the toxicity containing both, the test item and the reference item 28 % biodegradation was noted within 14 days and 32 % biodegradation was determined after 28 days of incubation.
- Results with reference substance:
- The reference item sodium benzoate was sufficiently degraded to 87 % after 14 days and to 90 % after 28 days of incubation.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28 -day exposure period the degradation of 0 % was found. The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
- Executive summary:
The test item was investigated for its ready biodegradability in a Manometric Respirometry Test according to OECD 301 F over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. All validity criteria were fulfilled. After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28 -day exposure period the degradation of 0 % was found. The reference item was sufficiently degraded to 87 % after 14 days, and to 90 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum. In the toxicity control containing both, the test item and the reference item, 28 % biodegradation was noted within 14 days and 32 % biodegradation was determined after 28 days of incubation. Thus, the test item can be assumed to be not inhibitory on the activated sludge microorganisms. In conclusion, the degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
Referenceopen allclose all
Table 1: Percentage Biodegradation of Test Item, of Sodium Benzoate and of the Toxicity Control
Time (Days) |
Percentage Biodegradation1 |
|||
Test Item |
Sodium benzoate |
Toxicity control2 |
||
Flask 1 [%] |
Flask 2 [%] |
Flask 5 [%] |
Flask 7 [%] |
|
1 |
0 |
0 |
28 |
7 |
2 |
-2 |
-2 |
40 |
14 |
3 |
-2 |
-2 |
51 |
18 |
4 |
-2 |
-2 |
61 |
22 |
5 |
-3 |
-3 |
69 |
23 |
6 |
-3 |
-3 |
74 |
24 |
7 |
-2 |
-3 |
76 |
25 |
8 |
-2 |
-3 |
79 |
25 |
9 |
-2 |
-3 |
81 |
26 |
10 |
-2 |
-3 |
83 |
27 |
11 |
-2 |
-2 |
84 |
27 |
12 |
-2 |
-2 |
85 |
27 |
13 |
-2 - |
2 |
87 |
28 |
14 |
-1 |
-1 |
87 |
28 |
15 |
-1 |
-1 |
87 |
28 |
16 |
-2 |
-1 |
86 |
28 |
17 |
-1 |
-1 |
86 |
29 |
18 |
0 |
0 |
87 |
29 |
19 |
0 |
0 |
87 |
29 |
20 |
1 |
1 |
90 |
30 |
21 |
1 |
1 |
90 |
30 |
22 |
1 |
1 |
89 |
31 |
23 |
2 |
2 |
90 |
31 |
24 |
1 |
2 |
90 |
31 |
25 |
1 |
2 |
90 |
31 |
26 |
1 |
2 |
89 |
31 |
27 |
1 |
2 |
89 |
31 |
28 |
1 |
2 |
90 |
32 |
1 based on ThODNH4
ThODNH4 of test item: 3.087 mg/mg
ThODNH4/ ThODNO3 of sodium benzoate: 1.666 mg/mg
Description of key information
After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28 -day exposure period the degradation of 0 % was found. The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
The test item was investigated for its ready biodegradability in a Manometric Respirometry Test according to OECD 301 F over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. All validity criteria were fulfilled. After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28 -day exposure period the degradation of 0 % was found. The reference item was sufficiently degraded to 87 % after 14 days, and to 90 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum. In the toxicity control containing both, the test item and the reference item, 28 % biodegradation was noted within 14 days and 32 % biodegradation was determined after 28 days of incubation. Thus, the test item can be assumed to be not inhibitory on the activated sludge microorganisms. In conclusion, the degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
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