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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-10-20 to 2010-12-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Aerobic activated sludge (micro-organisms from a domestic wastewater treatment plant) was supplied by the sewage works of Darmstadt, Germany.
The aerobic activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was resuspended in tap water and centrifuged again. This procedure was done three times. The sediment was resuspended in test water and aerated overnight. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 1.5 g dry material per litre were mixed with test water. This suspension was used for the experiment. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- ca. 103.5 mg/L
- Based on:
- ThOD/L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Test Water Reconstituted Test Water: Analytical grade salts were added to deionised water to prepare the following stock solutions:
a) 8.5 g KH2PO4, 21.75 g K2HPO4, 33.4 g Na2HPO4 x 2 H2O, 0.5 g NH4Cl filled up with deionised water to 1000 mL volume
b) 22.5 g MgSO4 x 7 H2O filled up with deionised water to 1000 mL volume
c) 36.4 g CaCl2 x 2 H2O filled up with deionised water to 1000 mL volume
d) 0.25 g FeCl3 x 6 H2O filled up with deionised water to 1000 mL volume
In order to avoid precipitation of iron hydroxide in the stock solution d) after storage and before use, one drop of concentrated HCl per litre was added.
10 mL of stock solution a) and 1 mL of the stock solutions b) to d) were combined and filled up to a final volume of 1000 mL with deionised water. The pH-value was 7.5 and therefore no adjustment was necessary.
The amounts of test item and reference item were directly weighed into the test flasks. No emulsifiers or solvents were used, but the solutions were dispersed by stirring to achieve a homogeneous solution of the test item.
There were to replicates of "test item" and "inoculum control", and one "Procedure Control", "Abiotic Control" and "Toxicity Control", respectively.
The prepared test flasks were incubated at 22 ± 1 °C. The pressure decrease in the reaction vessels was measured over complete experimental phase of 28 days using the BSB Sensomat system, Aqualytic Dortmund. The test flasks were closed gas-tight by a measuring head. Potassium hydroxide solution (45%) was used for trapping the produced carbon dioxide. The amount of O2 consumed by the activated sludge was calculated from the decrease of pressure in the reaction vessel.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Key result
- Parameter:
- other: ThOD NO3
- Value:
- 14
- Sampling time:
- 28 d
- Key result
- Parameter:
- other: ThOD NH4
- Value:
- 15
- Sampling time:
- 28 d
- Details on results:
- ThOD NH4
The occurrence of nitrification was considered but not ex-perimentally confirmed. If no nitrification occurs, the mean value for biodegradation was calculated to be 15% after 28 days. Therefore, the 10-day window criterion was not passed.
ThOD NO3
Based on ThODNO3 also no 10-day window was reached and the mean value for biodegradation was calculated to be 14% biodegradation after 28 days.
BOD5 / COD results
- Results with reference substance:
- The reference item sodium benzoate was sufficiently degraded to 75% after 14 days and to 82% after 28 days of incubation, thus confirming the suitability of the aerobic activated sludge inoculum used.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The degradation rate of Sa 57 (SAT No. 090073) did not reach 60% within the 10-day window and after 28 days of incubation when no nitrification is considered.
According to the test guideline, nitrification has to be con-sidered if nitrogen is part of the molecule. However, Sa 57 (SAT No. 090073) is considered not to be readily biodegradable. - Executive summary:
The test item Sa 57 (SAT No. 090073) was investigated for its ready biodegradability in a manometric respirometry test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the micro-organisms during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
The degradation rate of Sa 57 (SAT No. 090073) did not reach 60% within the 10-day window and after 28 days of incubation when no nitrification is considered.
According to the test guideline, nitrification has to be con-sidered if nitrogen is part of the molecule. However, Sa 57 (SAT No. 090073) is considered not to be readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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