Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 479-660-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Remarks:
- water
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
2 x 2 L with 100 mg/L were shaken with 20 rpm for 24 h and transferred to the gals aquarium.
- Controls:
dilution water
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): water
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): n.a.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.):
the test item sedimented and showed turbidity throughout the test
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Strain: Danio rerio
- Source:
a. Origin of parental fish: Aquarium Am Aegi, Hannover, Germany
b. fish used for the study: in house breed Dr. Noack Laboratorium, Sarstedt, Germany
- Length at study initiation (mean): 2.02 cm
- Weight at study initiation (mean): 0.07 g
- Method of breeding/maintenance:
Holding: was performed at the test facility at 23 +/- 2°C and diffuse light (0.1-10 µmol/m2s, natural photoperiod). The water was changed at least once per week. The dissolved oxygen concentration was more than 80% of the air saturation value. Only fish from a population with mortality < 5% within the last 7 days before start of the study were used in the test. No disease treatments were administered throughout holding and testing.
Water: Tap water of local origin was used for holding and testing. The water was filtered on activated charcoal and aerated for at least 24 h to remove chlorine.
Feeding: Food was provided 3 times per week. The amount of food was 4% of the fish body weight per feeding.
ACCLIMATION
none
FEEDING DURING TEST
The test fish were not fed until 24 h before the test started
Fish were not fed during the test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- 63 mg/L as CaCO3
- Test temperature:
- mean of 4 days: 21.6°C
- pH:
- mean of 4 days: 7.46
- Dissolved oxygen:
- mean of 4 days: 99%
- Nominal and measured concentrations:
- nominal: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel/Type:
Glass-aquaria loosely covered by glass tops
- fill volume:
approx. 4 L
- Aeration:
Gentle aeration provided
- Type of flow-through (e.g. peristaltic or proportional diluter):
static
- Renewal rate of test solution (frequency/flow rate):
one applicatin of test item at test initiation
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water of local origin, filtered on activated charcoal and aerated for at least 24 h to remove chlorine
- Intervals of water quality measurement: every 24 h
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: natural photoperiod
- Light intensity: 0.1 - 10 µE/m2 x s
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
every 24 h: mortality, behaviour
TEST CONCENTRATIONS
- Range finding study : no mortality at 100 mg/L (nominal) - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The test item sedimented and showed turbidity throughout the test. The nominal test item concentration of 100 mg/L caused no mortality or non-lethal effects.
The study met the validity criteria of the guideline:
- O2-saturation was >/= 60%
mortality in the control was 0% - Results with reference substance (positive control):
- n.a.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study the test substance did not cause any effects to zebrafish after 96 h when tested with a nominal concentration of 100 mg/L. The NOEC (0-96 h) is laid down as 100 mg/L.
- Executive summary:
The acute toxicity of the test substance to fish (zebrafrish) was determined according to OECD guideline 203.
A static limit test with a single test item concentration of 100 mg/L was conducted. Duration of the test was 96 h. 7 test organisms were exposed to the test concentration and control. Water quality parameters of pH-value and oxygen-saturation measured after 0, 24, 48, 72, and 96 h were determined to be within the acceptable limits. All effect values are based on the nominal concentration of the test item. No analysis was performed. Due to insolubility of the test item no specific analytical method was available.
The acute toxicity of the test substance is summarised as:
LC50 (96 h) > 100 mg/L
LC100 (96 h) > 100 mg/L
LC0 (96 h): 100 mg/L
NOEC (96 h): 100 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.