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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-10-03 to 2018-11-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- adopted July 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 23, Guidance document on aquatic toxicity testing of difficult substances and mixtures
- Version / remarks:
- 2000
- Deviations:
- not applicable
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Frequency:
At the start (t=0 h), after 24 h and the end of the test (t= 96 h).
While taking samples for analysis, care was taken not to include any floating layer, test item film or undissolved material in separate vessels.
Number of samples:
Sampling consisted of single samples per treatment.
Volume:
2 mL per sample;
Storage:
Samples were stored in a freezer (=-15°C) until analysis. - Vehicle:
- no
- Details on test solutions:
- Water Accommodated Fractions were prepared:
Individual loading rates were vigorously agitated on a magnetic stirrer for 6 days. On day 3 of stirring, pH was measured in case adjustment would be necessary (no adjustment was necessary:
pH of 8.0 ± 0.3). After 6 days of stirring, the obtained mixtures were allowed to settle for 1 day in order to allow separation of phases. Thereafter, the aqueous Water Accommodated
Fractions (WAFs) were collected by means of siphoning over glasswool (put into a funnel). Glasswool filtering was necessary to avoid a positive Tyndall effect. Glasswool was pre-saturated
with the test item beforehand. No Tyndall effect was observed in any prepared solution, and solutions were clear and colourless at the end of the preparation procedure.
Thereafter, the aqueous WAFs were used as test concentrations.
Based on preliminary results, the period of 6 days of stirring was used to ensure that
a) Concentration of the relevant transition metal stabilizes at the pH 8.0 ± 0.3 used for ecotoxicity testing to prevent any precipitation during the test;
b) Saturation of the aquatic phase with organic hydrolysis products stemming from aromatic phosphites is obtained. - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- Species Carp (Cyprinus carpio, Teleostei Cyprinidae) Linnaeus, 1758.
Source: Wageningen University and Research Centre, the Netherlands.
Fish length and weight: A representative number (10) of the batch of fish, from which fish were used for the present study, were weighed and measured prior to the start of the test.
Mean length of fish 3.0 ± 1.0 cm
Characteristics: F1 from a single parent-pair bred in UV-treated water. Healthy fish supplied with a health certificate.
Reason for selection: The system has been selected as an internationally accepted species. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 180 mg/L expressed as CaCO3
- Test temperature:
- 22 °C, constant within 2°C
- pH:
- 8.0 ± 0.3 at the start of the test
- Dissolved oxygen:
- > 60% of air saturation value
- Salinity:
- Not applicable
- Conductivity:
- See IUCLID section "Any other information on results incl. tables" below.
- Nominal and measured concentrations:
- For the initial range-finding and the actual limit test, the following water accommodated fractions (WAF, based on nominal loading rate) were individually prepared:
1.0 mg/L; 10 mg/L; 100 mg/L
Please see IUCLID section "Any other information on results incl. tables" below for information on analytical results. - Details on test conditions:
- Please see IUCLID section "Any other information on materials and methods incl. tables" below for details.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LL0
- Remarks:
- Based on Water Accommodated Fraction (WAF) approach
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks:
- and sub-lethal effects
- Duration:
- 96 h
- Dose descriptor:
- LL10
- Remarks:
- Based on Water Accommodated Fraction (WAF) approach
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks:
- and sub-lethal effects
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Remarks:
- Based on Water Accommodated Fraction (WAF) approach
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks:
- and sub-lethal effects
- Details on results:
- See IUCLID section "Any other information on results incl. tables" for details.
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- Not applicable - no effects observed.
- Sublethal observations / clinical signs:
These data are based on results from draft report and will be amended as soon as the final report will have been issued.
Details on Biological Data:
No mortality or any other sub-lethal symptoms were observed in the control and at any test concentrations of the range finding as well as the actual limit test (see table below).
Step 2 Catalyst (Stripped)
Loading Rate (mg/L)Initial
number
of fishCumulative mortality
Total
Mortality (%)2¼h
24h
48h
72h
96h
1.0 (range finding)
3
0
0
0
0
0
0
10 (range finding)
3
0
0
0
0
0
0
100 (range finding)
3
0
0
0
0
0
0
100 (limit test)
7
0
0
0
0
0
0
Control (limit test)
7
0
0
0
0
0
0
Details on Analytical Data:
At this time, analytical data are available for the range-finding test, only. Data on the final limit test will be amended as soon as they will become available.
1) Analysis of the relevant transition metal by ICP-MS
Time of sampling hours
Date of sampling
Date of analysis
Loading rate (WAF) [mg/L]
Concentration test item analysed [mg/L]
Relative to initial [%]
Transition metal concentration calculated from elemental fraction (0.8%) determined in study number 519586
[mg/L]
0
15 Oct 2018
24 Oct 2018
100
20.3
0.162
24
16 Oct 2018
24 Oct 2018
100
20.9
103
0.167
96
19 Oct 2018
24 Oct 2018
100
20.9
103
0.167
2) Analysis of the organics fraction by LC-UV at 210 nm
Time of sampling [hours]
Date of sampling
Date ofanalysis
Loading rate WAF [mg/L]
Concentration analysed [mg/L]
Relative to initial[%]
0
15 Oct 2018
31 Oct 2018
100
142
0
24
16 Oct 2018
31 Oct 2018
100
176
124
96
19 Oct 2018
31 Oct 2018
100
208
146
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity to freshwater fish was tested according to OECD 203 compliant with GLP. Due to the nature of Step 2 catalyst (UVCB), according to OECD 23 water accommodated fractions (WAF) were prepared.
Result:
LL0 (Cyprinus carpio; 96 h; static) = 100 mg/L;
LL10 (Cyprinus carpio; 96 h; static) > 100 mg/L
LL50 (Cyprinus carpio; 96 h; static) > 100 mg/L - Executive summary:
The acute toxicity to freshwater fish was tested according to OECD 203 compliant with GLP. The test organism was Cyprinus carpio (carp). Due to the nature of Step 2 catalyst (UVCB), according to OECD 23 water accommodated fractions (WAF) were prepared and based on the results of a range finding tests (neither mortalities nor any sublethal effects observed up to WAF 100 mg/L) a limit test was conducted. WAFs were prepared at 1, 10 and 100 mg/L nominal loading (range finding) and 100 mg/L nominal loading (limit test): individual loading rates were vigorously agitated on a magnetic stirrer for 6 days. Based on preliminary results, the period of 6 days of stirring was used to ensure that
a) Concentration of the relevant transition metal stabilizes at the pH 8.0 ± 0.3 used for ecotoxicity testing to prevent any precipitation during the test;
b) Saturation of the aquatic phase with organic hydrolysis products stemming from aromatic phosphites is obtained.
The presence of any non-dissolved test item fraction could be excluded before testing by measuring the Tyndall effect.
Analytical monitoring was performed for the relevant transition metal (ICP-MS) as well as the organic fraction (UPLC-UV at 210 nm). At the limit concentration of WAF 100 mg/L transition metal concentration was stable over the 96 hours of the test. Step 2 catalyst concentration equivalent of 20.3 mg/L was found in freshly prepared WAF (after 6 days of stirring and one day settling period) and 103% of this initial concentration was recovered after 24 and 96 hours (20.9 mg/L). This is equivalent to transition metal concentrations at 0, 24 and 96 hours of 162 µg/L, 167 and 167 µg/L, respectively.
With regard to the organics fraction monitored via UPLC-UV at 210 nm, an initial Step 2 catalyst concentration equivalent of 142 mg/L was measured at the limit concentration of WAF 100 mg/L. This increased during 96 hours of incubation by 46%. The reason for this increase is not clear. It could be due to further abiotic transformations (hydrolysis / oxidation) during the test. The results confirm in any case that WAF preparation succeeded in a relevant organic fraction present at the beginning and during the test.
For all concentrations tested (including the limit concentration of WAF 100 mg/L) neither in control groups nor in treatment groups any mortality or any other effects could be observed.
Result:
LL0 (Cyprinus carpio; 96 h; static) = 100 mg/L;
LL10 (Cyprinus carpio; 96 h; static) > 100 mg/L
LL50 (Cyprinus carpio; 96 h; static) > 100 mg/L
Reference
Description of key information
The acute toxicity to freshwater fish was tested according to OECD 203 compliant with GLP. Due to the nature of Step 2 catalyst (UVCB), according to OECD 23 water accommodated fractions (WAF) were prepared.
Result:
LL0 (Cyprinus carpio; 96 h; static) = 100 mg/L;
LL10 (Cyprinus carpio; 96 h; static) > 100 mg/L
LL50 (Cyprinus carpio; 96 h; static) > 100 mg/L
Key value for chemical safety assessment
Additional information
The acute toxicity to freshwater fish was tested according to OECD 203 compliant with GLP. The test organism was Cyprinus carpio (carp). Due to the nature of Step 2 catalyst (UVCB), according to OECD 23 water accommodated fractions (WAF) were prepared and based on the results of a range finding tests (neither mortalities nor any sublethal effects observed up to WAF 100 mg/L) a limit test was conducted. WAFs were prepared at 1, 10 and 100 mg/L nominal loading (range finding) and 100 mg/L nominal loading (limit test): individual loading rates were vigorously agitated on a magnetic stirrer for 6 days. Based on preliminary results, the period of 6 days of stirring was used to ensure that
a) Concentration of the relevant transition metal stabilizes at the pH 8.0 ± 0.3 used for ecotoxicity testing to prevent any precipitation during the test;
b) Saturation of the aquatic phase with organic hydrolysis products stemming from aromatic phosphites is obtained.
The presence of any non-dissolved test item fraction could be excluded before testing by measuring the Tyndall effect.
Analytical monitoring was performed for the relevant transition metal (ICP-MS) as well as the organic fraction (UPLC-UV at 210 nm). At the limit concentration of WAF 100 mg/L transition metal concentration was stable over the 96 hours of the test. Step 2 catalyst concentration equivalent of 20.3 mg/L was found in freshly prepared WAF (after 6 days of stirring and one day settling period) and 103% of this initial concentration was recovered after 24 and 96 hours (20.9 mg/L). This is equivalent to transition metal concentrations at 0, 24 and 96 hours of 162 µg/L, 167 and 167 µg/L, respectively.
With regard to the organics fraction monitored via UPLC-UV at 210 nm, an initial Step 2 catalyst concentration equivalent of 142 mg/L was measured at the limit concentration of WAF 100 mg/L. This increased during 96 hours of incubation by 46%. The reason for this increase is not clear. It could be due to further abiotic transformations (hydrolysis / oxidation) during the test. The results confirm in any case that WAF preparation succeeded in a relevant organic fraction present at the beginning and during the test.
For all concentrations tested (including the limit concentration of WAF 100 mg/L) neither in control groups nor in treatment groups any mortality or any other effects could be observed.
Result:
LL0 (Cyprinus carpio; 96 h; static) = 100 mg/L;
LL10 (Cyprinus carpio; 96 h; static) > 100 mg/L
LL50 (Cyprinus carpio; 96 h; static) > 100 mg/L
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