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EC number: 701-300-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2 December 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances):
1-Litre test bottles were filled with 200 ml of test substance mixtures in Milli-RO water (tap water purified by reverse osmosis) with initial loading rates of 2.5 times the final loading rate. These mixtures were stirred in closed dark brown bottles for approximately 24 hours. The appearance of the loading rates varied from clear and colorless at the lowest loading rate to opalescent with undissolved test substance particles (as precipitate and floating at the surface) at the highest loading rate.
Subsequently, 16 ml synthetic sewage feed, 250 ml sludge and Milli-RO water up to 500 ml were added resulting in the required loading rates. Optimal contact between the test substance and test organisms was ensured applying continuous aeration and stirring. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Preparation of inoculum for exposure:
The sludge was coarsely sieved, washed and diluted with ISO-medium. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (3.0 g/l of sludge, as used for the test). The pH was 7.7 on the day of testing. The batch of sludge was used one day after collection; therefore 50 ml of synthetic sewage feed was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
- Initial biomass concentration: Number of micro-organisms was determined as the amount of Mixed Liquor Suspended Solids (MLSS) per litre test medium = 1.5 g/L of sludge - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- None
- Hardness:
- No information.
- Test temperature:
- 19.8 - 21.1 ºC
- pH:
- 7.7-7.8 before addition of sludge
7.7 - 8.3 after 3 hrs contact time - Dissolved oxygen:
- 8.0-8.8 mg O2/L at the start of the study
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- Not applicable.
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: all-glass, 1 liter, filled with 500 ml test solution
- Aeration: yes, using a pipette
- No. of organisms per vessel: 250 ml sludge
- No. of vessels per concentration (replicates): 3 for highest loading rate, 1 for lower loading rates
- No. of vessels per control (replicates): 6
- 1 abiotic controland 1 higest loading rate + nitrification inhibitor
TEST MEDIUM / WATER PARAMETERS
Adjusted ISO medium, formulated using RO-water (tapwater purified by reverse osmosis) with the following composition:
CaCl2.2H2O 211.5 mg/l
MgSO4.7H2O 88.8 mg/l
NaHCO3 46.7 mg/l
KCl 4.2 mg/l
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen recording with oxygen probes connected to a BlueBox, a multichannel measuring and controlling system for a period of approximately 10 minutes. During measurement, the sample was not aerated but continuously
stirred on a magnetic stirrer.
TEST CONCENTRATIONS
In a combined limit/range-finding test loading rates of 10, 100 and 1000 mg/l were tested. The highest loading rate was tested in triplicate, lower loading rates consisted of one replicate. In addition, a blankcontrol (6 replicates) was included. Furthermore, an abiotic control and the highest loading rate with a nitrification inhibitor were tested. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- In the combined limit/range-finding test no statistically significant inhibition of the respiration rate of the sludge was recorded at a loading rate of 1000 mg CAT per litre.
Furthermore, there was no oxygen uptake from abiotic processes and the results at 1000 mg/l with a nitrification inhibitor showed that the heterotrophic inhibition of the respiration rate was comparable to the total inhibition. - Results with reference substance (positive control):
- The EC50 of 3,5-dichlorophenol (8.1 mg/l) was in the accepted range of 2 to 25 mg/l for total respiration.
- Reported statistics and error estimates:
- The NOEC was based on statistical analysis of the data. Data obtained for the test concentrations were compared with those obtained for the control using TOXSTAT Release 3.5, 1996, D.D. Gulley, A.M. Boelter, H.L. Bergman.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the combined limit/range-finding test no statistically significant inhibition of the respiration rate of the sludge was recorded at a loading rate of 1000 mg CAT per litre.
Under the conditions of this present test CAT was not toxic to waste water (activated sludge) bacteria at a loading rate of 1000 mg/l (NOEC).
Reference
Flask |
Conc. / loading rate (mg/l) |
Temp (°C) |
pH before addition of sludge |
pH after 3 h contact time |
Oxygen conc. at the start (~ mg O2/l) |
Respi- ration rate (mg O2/l h) |
Respi- ration rate (mg O2/g h)¹ |
% Inhibition respiration relative to the control (mean value) |
C 1 |
0 |
21.1 |
7.8 |
7.9 |
8.6 |
38 |
25 |
|
C 2 |
0 |
20.3 |
7.8 |
7.9 |
8.6 |
39 |
26 |
|
C 3 |
0 |
20.5 |
7.8 |
7.9 |
8.0 |
36 |
24 |
|
C 4 |
0 |
20.4 |
7.8 |
8.0 |
8.0 |
33 |
22 |
|
C 5 |
0 |
20.6 |
7.8 |
8.0 |
8.0 |
34 |
23 |
|
C 6 |
0 |
20.6 |
7.8 |
8.0 |
8.0 |
37 |
25 |
|
Mean |
36 |
24 |
||||||
SD |
2 |
2 |
||||||
CV (%) |
6 |
6 |
||||||
CN (with ATU) |
0 |
20.9 |
7.8 |
8.2 |
8.8 |
21 |
14 |
|
R 1 |
5.0 |
21.1 |
7.8 |
8.0 |
8.8 |
23 |
15 |
36 |
R 2 |
12 |
20.9 |
7.8 |
8.1 |
8.6 |
13 |
9 |
64 |
R 3 |
30 |
20.3 |
7.7 |
8.2 |
8.8 |
7 |
5 |
81 |
T 1 |
10 |
19.8 |
7.7 |
7.9 |
8.1 |
34 |
23 |
6 |
T 2 |
100 |
20.6 |
7.7 |
7.9 |
8.2 |
38 |
25 |
-5 |
T3a |
1000 |
20.1 |
7.7 |
7.8 |
8.0 |
39 |
26 |
-8 |
T3b |
1000 |
20.8 |
7.7 |
7.7 |
8.2 |
38 |
25 |
-5 |
T3c |
1000 |
20.1 |
7.7 |
7.7 |
8.3 |
41 |
27 |
-13 |
T3 mean |
39 |
26 |
-9 |
|||||
TA (abiotic) |
1000 |
20.6 |
7.7 |
7.4 |
8.8 |
1 |
1 |
97 |
TN (with ATU) |
1000 |
20.9 |
7.7 |
8.3 |
8.1 |
19 |
13 |
102 |
C: Blank Control
CN: Nitrification control
R: Reference substance, 3,5-dichlorophenol
T: Test substance, CAT
CV: Coefficient of variation
ATU N-allylthiourea
¹ The amount of suspended solids in the final test mixture was 1.5 g/l.
2 Relative to CN
Description of key information
CAT was tested in an activated sludge respiration inhibition test (OECD 209). The substance was not toxic to waste water (activated sludge) bacteria at a loading rate of 1000 mg/l (NOEC).
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
CAT was tested in an activated sludge respiration inhibition test (OECD 209). The substance was not toxic to waste water (activated sludge) bacteria at a loading rate of 1000 mg/l (NOEC).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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