Dipentaerythritol hexaesters of 3,5,5-trimethylhexanoic and n-heptanoic acids

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23 Mar - 25 Mar 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Remarks:

The Department of Health of the Government of the United Kingdom, date of inspection 23 March 1998

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control - 100 mg/L
- Sampling method: Water samples were taken from the control and the 100 mg/L loading rate WAF test group (replicates R1-R2 and R3-R4 pooled) at 0 and 48 h. Duplicate samples were taken at each occasion.
- Sample storage conditions before analysis: Stored frozen (approx. -20 °C) for further analysis.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: An amount of test material (500 mg) was added to the vortex of 5 L of reconstituted water and stirred for 23 h to give the 100 mg/L loading rate. The stirring was stopped after 23 h and the mixture allowed to stand for 1 h. The water accommodated fraction (WAF) was then removed by filtration through 0.45 µm filters to give the 100 mg/L loading rate WAF.
- Controls: The control group was maintained under identical conditions but not exposed to the test material.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: supplied by the Institut National de Recherche Chimique Appliquée (IRCHA), France
- Age at study initiation: < 24 h
- Method of breeding: Adult Daphnia were maintained in polypropylene vessels containing approx. 2 L of reconstituted water in a temperature controlled room at 21 °C with a 16 h light and 8 h darkness cycle with 20 min dawn and dusk transition periods. The cultures were fed with a suspension of mixed algae (predominantly Chlorella spp.). Culture conditions ensured that reproduction was by parthenogenesis.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

21 °C

pH:

7.9-8.0

Dissolved oxygen:

8.0-8.3 mg O2/L

Nominal and measured concentrations:

Nominal concentrations: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: jar
- Type: covered to reduce evaporation
- Material: glass; Size: 250 mL; Fill volume: 250 mL
- No. of organisms per vessel: 10
- No. of vessels per concentration: 4
- No. of vessels per control: 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water

OTHER TEST CONDITIONS
- Photoperiod: 16 h light and 8 h darkness cycle with 20 min dawn and dusk transition periods

EFFECT PARAMETERS MEASURED: Immobilisation was measured after 24 and 48 h test duration.

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 10 and 100 mg/L loading rate WAFs
- Results used to determine the conditions for the definitive study: No immobilisation was observed.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

(WAF)

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOELR

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

(WAF)

Basis for effect:

mobility

Details on results:

- Mortality of control: No mortality was observed
- Effect concentrations exceeding solubility of substance in test medium: The effect concentration exceeded the water solubility of the test substance.

No effects in the range of the water solubility of the test substance on immobilisation of Daphnia magna were observed after the study period of 48 h. This resulted in an EL50 of > 100 mg/L (nominal). Chemical analysis of the 100 mg/L loading rate WSFs at 0 and 48 h showed the measured test concentrations to be less than or at the limit of quantification of the analytical method. Increasing the time of stirring to 192 h did not result in a significant increase in the amount of carbon in the WSF with respect to the control values.

Table 1: Chemical analysis of test loading rates

Samples	Nominal loading rate [mg/L]	Concentration found [mg/L]
0 h	control	< LOQ
	100 R1-R2	< LOQ
	100 R3-R4	0.00613
48 h	control	< LOQ
	100 R1-R2	< LOQ
	100 R3-R4	< LOQ

Validity criteria fulfilled:

yes

Conclusions:

EL50 (48 h) > 100 mg/L (nominal) (Daphnia magna, OECD 202)
No effects up to the limit of the water solubility of the substance.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

In accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5 a read across to the structurally similar analogue substance Dipentaerythritol with fatty acids C5 and C9 iso (CAS 647028-25-9) was conducted to fulfil the data requirements according to Annex VII of Dipentaerythritol hexaesters of 3,5,5-trimethylhexanoic and n-heptanoic acids (CAS 1379424-11-9) in regard to toxicity to invertebrates.
This read-across is justified in detail in the overall summary (IUCLID section 6.1) and within the analogue justification in IUCLID section 13. The source substance consists of the same alcohol component (dipentaerythritol) and similar fatty acid chain lengths as the target substance (C7, C9-iso for the target substance compared to C5, C9-iso for the source substance).

The study with the read across substance dipentaerythritol with fatty acids C5 and C9iso was conducted under static conditions according to OECD 202 using Daphnia magna as test organism. Nominal concentration of 100 mg/L (prepared as WAF) was tested. Analytical monitoring showed that the measured test concentration was less than the limit of quantification. After 48 h no effects were observed. No mortality and no signs of intoxication were observed. Therefore, this study resulted in a 48 h EL50 of > 100 mg/L (nominal). The NOELR was derived to be ≥ 100 mg/L (nominal).

Based on the result from the structurally related substance, characterized by a similar ecotoxicological profile and in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5, it can be concluded that Dipentaerythritol hexaesters of 3,5,5-trimethylhexanoic and n-heptanoic acids will not exhibit toxic effects to invertebrates up to the limit of water solubility.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

WAF

Basis for effect:

mobility

Remarks:

no mortality and no signs of intoxication were observed

Key result

Duration:

48 h

Dose descriptor:

NOELR

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

WAF

Basis for effect:

mobility

Remarks:

no mortality and no signs of intoxication were observed

Conclusions:

The short-tem toxicity to aquatic invertebrates with source substance Dipentaerythritol with fatty acids C5 and C9iso (OECD 202 using Daphnia magna as test orngnism) showed no mortality and no signs of intoxication after 48 h. This study resulted in a 48 h EL50 of >100 mg/L (nominal). The NOELR was derived to be ≥ 100 mg/L (nominal).

Based on the results from the structurally related source substance, it can be concluded that the target substance Dipentaerythritol hexaesters of 3,5,5-trimethylhexanoic and n-heptanoic acids will not exhibit toxic effects to invertebrates upu to the limit of water solubility.

Description of key information

EL50 (48 h) > 100 mg/L (nominal) (Daphnia magna, OECD 202)
No effects up to the limit of the water solubility of the source substance.

Based on the result from the structurally related substance, characterized by a similar ecotoxicological profile and

in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5, it can be concluded that Dipentaerythritol hexaesters of 3,5,5-trimethylhexanoic and n-heptanoic acids will not exhibit short-term toxic effects to aquatic invertebrates up to the limit of water solubility.