Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 221-361-2 | CAS number: 3077-27-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 - 22 June 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Reconstructed Human Epidermis (RHE) Test Method)
- Version / remarks:
- 29 July 2016
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: EU-Method B.40 BIS (in vitro skin corrosion: human skin model test)
- Version / remarks:
- 30 May 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Kaiser-Friedrich-Straße 7, 55116 Mainz, Germany
Test material
- Reference substance name:
- 4-(octadecylamino)-4-oxoisocrotonic acid
- EC Number:
- 221-361-2
- EC Name:
- 4-(octadecylamino)-4-oxoisocrotonic acid
- Cas Number:
- 3077-27-8
- Molecular formula:
- C22H41NO3
- IUPAC Name:
- 4-(octadecylamino)-4-oxoisocrotonic acid
- Test material form:
- solid
Constituent 1
In vitro test system
- Test system:
- human skin model
- Source species:
- human
- Cell type:
- non-transformed keratinocytes
- Cell source:
- other: MatTek In Vitro Life Science Laboratories, Bratislava, Slovakia
- Source strain:
- other: EpiDermTM; reconstructed three-dimensional human epidermis (EPI-200-SCT)
- Vehicle:
- unchanged (no vehicle)
- Details on test system:
- TEST METHOD:
The model represents a reconstructed three-dimensional skin model based on normal human-derived epidermal keratinocytes which have been cultured to form a multilayered epidermis including basal, spinous and granular layers, and a multi-layered stratum corneum. Irritant materials are identified by their ability to penetrate the stratum corneum and to damage the underlaying cell layers which is determined through a decrease in cell viability as determined by MTT reduction assay.
ADAPTATION TO CELL CULTURE CONDITIONS:
Upon receipt, tissues were transferred into 6-well plates containing 900 µL pre-warmed assay medium per well and preincubated in a humidified incubator for at least 1 h (37 ± 1 °C, 5% CO2) before use.
INCUBATION CONDITIONS (INCUBATOR):
- Temperature (°C): 37 ± 1
- CO2 gas concentration (%): 5
- Humidity: maximum
RECONSTRUCTED HUMAN EPIDERMIS (RHE) TISSUE :
- Model used: EpiDermTM EPI-200-SCT
- Tissue batch number(s): 25822
- Delivery date: 20 June 2017
TEMPERATURE USED FOR TEST SYSTEM :
- Temperature used during treatment / exposure: room temperature (3 min exposure) and 37 +/- 1 °C, 5.0 +/- 0.5% CO2 (1 h exposure)
- Temperature of post-treatment incubation (if applicable): 37 +/- 1 °C, 5.0 +/- 0.5% CO2
REMOVAL OF TEST MATERIAL AND CONTROLS :
-Volume and number of washing steps: thoroughly rinsed with Dulbecco's phosphate-buffered saline and blotted with sterile cellulose tissue
- Observable damage in the tissue due to washing: no
- Modifications to validated SOP: no
CELL VIABILITY MEASUREMENTS:
For determining alterations in cell viability, MTT reduction assays were performed. Therefore, tissues were incubated in 300 µL pre-warmed MTT solution for 3 h at 37 ± 1 °C and 5% CO2. After aspiration of the MTT solution, tissues were washed 3 times in phosphate buffered saline followed by tissue drying. Extraction of the formazan product was carried out in 2 mL isopropanol. The optical density was measured at 570 nm wave length in a plate spectrophotometer.
MTT DYE USED TO MEASURE TISSUE VIABILITY AFTER TREATMENT / EXPOSURE :
- MTT concentration: 5 mg/mL
- Incubation time: 3 h, 37 +/- 1 °C, 5 +/- 0.5 % CO2
- Wavelength: 570 nm
NUMBER OF REPLICATE TISSUES: 2
NUMBER OF INDEPENDENT TEST SEQUENCES / EXPERIMENTS TO DERIVE FINAL PREDICTION: 1
PREDICTION MODEL / DECISION CRITERIA:
- The test substance is considered to be corrosive to skin if the viability after 3 minutes exposure is less than 50%, or if the viability after 3 minutes exposure is greater than or equal to 50 % and the viability after 1 hour exposure is less than 15%.
- The test substance is considered to be non-corrosive to skin if the viability after 3 minutes exposure is greater than or equal to 50% and the viability after 1 hour exposure is greater than or equal to 15%. - Control samples:
- yes, concurrent negative control
- yes, concurrent positive control
- Amount/concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): approx. 25 mg
NEGATIVE CONTROL
- Amount(s) applied (volume or weight): 50 µL
POSITIVE CONTROL
- Amount(s) applied (volume or weight): 50 µL
- Concentration (if solution): 8N - Duration of treatment / exposure:
- 3 and 60 min
- Duration of post-treatment incubation (if applicable):
- 1 h
- Number of replicates:
- The test was performed in duplicates for each test or control group and treatment period (3 and 60 min).
Test animals
- Species:
- other: in vitro system
Test system
- Type of coverage:
- other: in vitro system
Results and discussion
In vitro
Resultsopen allclose all
- Irritation / corrosion parameter:
- % tissue viability
- Remarks:
- test item
- Run / experiment:
- 3 min
- Value:
- 99.9
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Irritation / corrosion parameter:
- % tissue viability
- Remarks:
- test item
- Run / experiment:
- 60 min
- Value:
- 107.4
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Irritation / corrosion parameter:
- % tissue viability
- Remarks:
- positive control
- Run / experiment:
- 3 min
- Value:
- 19.5
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Irritation / corrosion parameter:
- % tissue viability
- Remarks:
- positive control
- Run / experiment:
- 60 min
- Value:
- 7.8
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Other effects / acceptance of results:
- OTHER EFFECTS:
- Visible damage on test system: no
- Direct-MTT reduction: no, as assessed in a suitable pre-experiment
- Colour interference with MTT: no, as assessed in a suitable pre-experiment
ACCEPTANCE OF RESULTS:
- Acceptance criteria met for negative control: yes, OD 1.8 (3 min) and 1.6 (1 h) (criterion: OD ≥ 0.8 and ≤ 2.8)
- Acceptance criteria met for positive control: yes, % tissue viability after 1 h: 7.8 (criterion: < 15%)
- Range of historical values if different from the ones specified in the test guideline: values for negative control and for positive control were within the range of historical data of the test facility
The values of the negative control met the required acceptability criterion of mean OD ≥ 0.8 and ≤ 2.8 for both treatment intervals, thus showing the quality of the tissues. The positive control has met the validity criterion too, thus ensuring the validity of the test system. For these reasons, the result of the test is considered valid.
Any other information on results incl. tables
Table 1: Absorbance values blank isopropanol (OD 570nm)
Replicate |
1 |
2 |
3 |
4 |
5 |
6 |
Mean 0.038 |
Absorbance |
0.038 |
0.037 |
0.039 |
0.039 |
0.038 |
0.038 |
|
Replicate |
7 |
8 |
9 |
10 |
11 |
12 |
|
Absorbance |
0.039 |
0.038 |
0.038 |
0.039 |
0.038 |
0.037 |
Table 2: Absorbance Values (OD 570nm) for negative control, test item and positive control
Incubation |
Negative Control |
Test Item |
Positive Control |
|||
|
Tissue 1 |
Tissue 2 |
Tissue 1 |
Tissue 2 |
Tissue 1 |
Tissue 2 |
3 min |
1.797 |
1.826 |
1.830 |
1.822 |
0.380 |
0.385 |
1.853 |
1.786 |
1.761 |
1.813 |
0.381 |
0.384 |
|
1.791 |
1.776 |
1.771 |
1.818 |
0.378 |
0.385 |
|
1 h |
1.602 |
1.600 |
1.741 |
1.720 |
0.167 |
0.157 |
1.596 |
1.607 |
1.767 |
1.679 |
0.165 |
0.156 |
|
1.597 |
1.618 |
1.722 |
1.686 |
0.166 |
0.154 |
Table 3: Mean Absorbance Values of the 3 min Experiment
Designation |
Negative Control |
Test Item |
Positive Control |
Mean – blank (tissue 1) |
1.776 |
1.749 |
0.342 |
Mean – blank (tissue 2) |
1.758 |
1.780 |
0.347 |
Mean of the two tissues |
1.767 |
1.764 |
0.344 |
RSD |
0.7% |
1.2% |
1.0% |
Table: 4: Mean Absorbance Values of the 1 h Experiment
Designation |
Negative Control |
Test Item |
Positive Control |
Mean – blank (tissue 1) |
1.560 |
1.705 |
0.128 |
Mean – blank (tissue 2) |
1.570 |
1.657 |
0.118 |
Mean of the two tissues |
1.565 |
1.681 |
0.123 |
RSD |
0.5% |
2.0% |
6.0% |
Table 5: % Tissue Viability
Test Item |
Positive Control |
Incubation |
99.9 % |
19.5 % |
3 min |
107.4 % |
7.8 % |
1 h |
Table 6: Historical Data
Parameter |
Optical Density Negative Control |
Optical Density Negative Con- trol |
% Tissue viability Positive Control |
% Tissue viability Positive Control |
Incubation Time |
3 min. |
1 h |
3 min. |
1 h |
Mean |
1.956 |
1.905 |
25.1 % |
12 % |
Standard Deviation |
0.275 |
0.220 |
6.8 % |
3.8 % |
Range |
1.197 - 3.077 |
1.377 - 2.571 |
9.6 - 57.3 % |
4.1 - 24.2 % |
Study17051601G820 |
1.767 |
1.565 |
19.5 |
7.8 |
Applicant's summary and conclusion
- Interpretation of results:
- other: not corrosive
- Conclusions:
- There is regulatory acceptance in the EU that a substance can be considered corrosive based on a positive result in the human epidermis model test (OECD 431). Negative in vitro corrosivity responses are not conclusive with respect to non-classification or classification as irritant. Therefore, the result obtained needs to be supported by additional data in order to conclude the hazard assessment and determination of the classification and labelling.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.