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EC number: 701-298-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
- Biodegradation
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From2017-06-01 to 2017-10-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Dialkyl C18 and C18-unsaturated phosphonates
- EC Number:
- 701-298-1
- Cas Number:
- 64051-29-2
- Molecular formula:
- Not applicable for a UVCB Substance
- IUPAC Name:
- Dialkyl C18 and C18-unsaturated phosphonates
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The batch of Alkenyl phosphonate tested was a colourless liquid UVCB (Unknown, of Variable Composition, or of Biological origin). No correction was made for the purity/composition of the test item.
Since the test item was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 10 g/L, weighed amounts were added to the dark brown test-bottles containing 200 mL Milli-RO water (tap water purified by reverse osmosis; Millipore Corp., Bedford, Mass., USA). The test item – Milli-RO water mixtures were magnetically stirred for a short period and subsequently, 16 mL synthetic medium made up to 50 mL with Milli-RO water and 250 mL sludge were added resulting in the required loading rates. Optimal contact between the test item and test organisms was ensured by applying continuous aeration and stirring.
Any residual volumes were discarded.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: Municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Preparation of inoculum for exposure: The sludge was coarsely sieved (1 mm) and allowed to settle. The supernatant was removed and ISO-medium was added. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (3.0 g/L of sludge, as used for the test). The pH was 7.6 on the day of testing. The batch of sludge was used one day after collection; therefore 50 mL of synthetic medium (=sewage feed) was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
Medium Adjusted ISO-medium, formulated using RO-water (tap water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands) with the following composition:
CaCl2.2H2O 211.5 mg/L
MgSO4.7H2O 88.8 mg/L
NaHCO3 46.7 mg/L
KCl 4.2 mg/L
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Hardness:
- No data
- Test temperature:
- 20 °C to 21 °C
- pH:
- 7.2 to 8.2
- Dissolved oxygen:
- The aeration was adjusted in such a way that the dissolved oxygen concentration at the start was above 60-70% saturation (60% of air saturation is > 5 mg/L at 20°C) and to maintain the sludge flocs in suspension
- Salinity:
- not applicable, fresh water
- Nominal and measured concentrations:
- Nominal concnetration: 10, 100 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: All glass open bottles/vessels.
- Aeration: The aeration was adjusted in such a way that the dissolved oxygen concentration at the start was above 60-70% saturation (60% of air saturation is > 5 mg/L at 20°C) and to maintain the sludge flocs in suspension.
- No. of vessels per concentration (replicates):
Control: 6
Reference: 4
Low and medium dose: 1
Hih dose: 3
Abiotic control: 1
- Biomass loading rate: 3 g/L
TEST MEDIUM
Medium Adjusted ISO-medium, formulated using RO-water (tap water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands) with the following composition:
CaCl2.2H2O 211.5 mg/L
MgSO4.7H2O 88.8 mg/L
NaHCO3 46.7 mg/L
KCl 4.2 mg/L
Synthetic medium (=sewage feed)
16 g peptone
11 g meat extract
3 g urea
0.7 g NaCl
0.4 g CaCl2.2H2O
0.2 g MgSO4.7H2O
2.8 g K2HPO4
Dissolved in Milli-RO water, made up to 1 litre and filtered. The pH was within 7.5 ± 0.5.
Performance of the test
The synthetic medium (16 mL) made up to 50 mL with Milli- RO and 200 mL test item solution were mixed (total volume 250 mL). The pH was determined. Thereafter, 250 mL activated sludge was added. This was the start of the test.
After the 3-hour contact time, the oxygen consumption was recorded for a period of approximately 10 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer. - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- other: NOELR
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- other: ELR50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- - Results with reference substance valid?: Yes
Any other information on results incl. tables
Results: Respiration Rate/Inhibition, pH Values
Replicate |
Loading rate (T) Concentration (R) (mg/L) |
pH |
Respiration rate |
% Inhibition respiration rate (mean value) |
||
Start |
End |
(mg O2/L.h) |
(mg O2/g.h)¹ |
|||
C 1 |
0 |
7.6 |
7.9 |
38.85 |
25.90 |
|
C 2 |
0 |
7.6 |
7.8 |
28.91 |
19.27 |
|
C 3 |
0 |
7.5 |
7.6 |
42.43 |
28.29 |
|
C 4 |
0 |
7.5 |
7.7 |
35.35 |
23.57 |
|
C 5 |
0 |
7.5 |
7.6 |
39.16 |
26.11 |
|
C 6 |
0 |
7.5 |
7.7 |
35.22 |
23.48 |
|
C Mean |
|
|
|
36.65 |
24.44 (RC) |
|
SD |
|
|
|
4.65 |
3.10 |
|
CV (%) |
|
|
|
13 |
13 |
|
R 1 |
1.0 |
7.6 |
8.0 |
26.89 |
17.93 |
26.64 |
R 2 |
3.2 |
7.6 |
8.2 |
17.68 |
11.79 |
51.76 |
R 3 |
10 |
7.6 |
8.1 |
9.35 |
6.23 |
74.49 |
R 4 |
32 |
7.6 |
8.1 |
6.31 |
4.21 |
82.78 |
T 1 |
10 |
7.5 |
7.6 |
37.49 |
24.99 |
-2.28 |
T 2 |
100 |
7.5 |
7.6 |
35.35 |
23.57 |
3.56 |
T 3a |
1000 |
7.4 |
7.5 |
38.33 |
25.55 |
-4.57 |
T 3b |
1000 |
7.4 |
7.5 |
36.51 |
24.34 |
0.39 |
T 3c |
1000 |
7.4 |
7.7 |
30.38 |
20.25 |
17.12 |
T3 Mean |
|
|
35.07 |
23.38 (RT) |
4.31 (IT) |
|
TA |
1000 |
7.4 |
7.2 |
0.00# |
0.00# |
100.00 |
C: Control ¹
R: Reference item, 3,5-dichlorophenol
T: Test item, Alkenyl phosphonate
TA: Abiotic control of Alkenyl phosphonate
SD: Standard deviation
CV: Coefficient of variation
#: No respiration, therefore expressed as 0 mg O2/L.h (see paragraph 6.1)
¹ The amount of suspended solids in the final test mixture was 1.5 g/L.
RC: Total respiration in the control
RT: Total respiration with Alkenyl phosphonate
IT: % inhibition of total respiration relative to RC
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this present test Alkenyl phosphonate was not toxic to waste water bacteria (activated sludge) at a loading rate of 1000 mg/L (NOELR).
The ELR50 was above 1000 mg/L. - Executive summary:
The objective of the study was to evaluate Alkenyl phosphonate for its ability to adversely affect aerobic microbial treatment plants and, if possible, to determine the ELR50and/or the no-observed effect loading rate (NOELR).
The influence of Alkenyl phosphonate on the respiration rate of activated sludge was investigated after a contact time of 3 hours.
The study procedures described in this report were based onthe OECD guideline No. 209, 2010.
The batch of Alkenyl phosphonate tested was a colourless liquid (UVCB). No correction was made for the purity/composition of the test item.
The test item was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 10 g/L, volumes corresponding to final concentrations of 10, 100 and 1000 mg/L have been added to the test-bottles containing Milli-RO water. The test item – Milli-RO water mixtures were magnetically stirred for a short period of 30 minutes and subsequently, synthetic medium, sludge and Milli-RO water were added resulting in the required loading rates. Optimal contact between the test item and test medium was ensured by applying continuous aeration and stirring during the 3-hour exposure period. Thereafter, oxygen consumption was recorded for approximately 10 minutes.
In a combined limit/range-finding test loading rates of 10, 100 and 1000 mg/L were tested. The highest loading rate was tested in triplicate, lower loading rates consisted of one replicate. Furthermore, at 1000 mg/L an abiotic control (1 replicate) was tested.Responses were compared to the controls.
Nostatistically significant inhibition of the respiration rate of the sludge was recordedat a loading rate of 1000 mg Alkenyl phosphonate per litre.Therefore, the ELR50was above the highest concentration tested (1000 mg/L). There was no oxygen uptake from abiotic processes.
The batch of activated sludge was tested for sensitivity with the reference item 3,5- dichlorophenol, and showed normal sensitivity.
The study met the acceptability criteria prescribed by the study plan and was considered valid.
Under the conditions of this present test, Alkenyl phosphonate was not toxic to waste water (activated sludge) bacteria at a loading rate of 1000 mg/L (NOELR).
The ELR50was above 1000 mg/L.
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