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EC number: 271-547-2 | CAS number: 68585-02-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-06-28 to 2018-07-27
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal sewage treatment plant, 31137 Hildesheim, Germany
- Laboratory culture/ Method of cultivation:
The activated sludge was washed twice with chlorine free tap water.
After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration with CO2 free air until one day before test start (1 day).
- Pretreatment: no
- Concentration of sludge: 14 mL/L of the mixture above were used to initiate inoculation. () 25.0 mg/L dw)
- Initial cell/biomass concentration: approx. 10E7 - 10E8 CFU/L
- Water filtered: no information - Duration of test (contact time):
- 28 d
- Initial conc.:
- 10.7 mg/L
- Based on:
- other: Carbon content (TOC) in test vessel
- Initial conc.:
- 16 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral salts medium acc. to OECD 301 B / CO2 Evolution Test
- Additional substrate: no
- Solubilising agent (type and concentration if used): no
- Test temperature: 22±2 °C measured 20.5 - 23 °C
- pH: 7.6
- Aeration of dilution water: 30 - 100 mL/min
- Suspended solids concentration: not mentioned
- Continuous darkness: Low light conditions (brown glass bottles)
TEST SYSTEM
- Culturing apparatus: 5000ml brown glas
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: vessels were aerated for 24 h with CO2 free air; 30 - 100 mL/min
- Measuring equipment:
pH-Meter, Multi 350i, WTW
Thermohygrograph, LUFFT
Ultrasonic bath, SONOREX, BANDELIN
Flow meter, Typ DK 800 PV, KROHNE DUISBURG
Analytical balance, SARTORIUS
Balance, KERN
Dispensette, BRAND
Digital Buret continuous RS, VITLAB
Medo Compressor, REBIE
Magnetic stirrer Mono, VARIOMAG
Multipette X-Stream, EPPENDORF
Various Pipettes
Test performed in open system: no: CO2 evolution test
- Details of trap for CO2 and volatile organics if used: according to guideline
SAMPLING
- Sampling frequency:
Determination of CO2 was carried out by titration subsequent to complete adsorption of the released CO2 in an alkaline solution (0.0125 mol/L Ba(OH)2).
For each titration the first gas wash bottle was removed and a new bottle was connected to the last one.
Back titration of the residual Ba(OH)2 with 0.05 N HCl was carried out three times a week during the first ten days and thereafter twice weekly.
On day 28 the pH of all solutions was measured prior to acidification.
- Sampling method: no sampling
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 vessels for inocculum control
- Toxicity control: one vessel - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 3
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- Under the test conditions Octadecanoic acid, reaction products with acetic acid and tetraethylenepentamine is classified as not readily biodegradable within the 28 day period of the study.
- Executive summary:
The ready biodegradability ofOctadecanoic acid, reaction products with acetic acid and tetraethylenepentaminewas determined with a non-adapted activated sludge over a test period of 28 days in the Modified Sturm Test. The study was conducted according to OECD 301 B.The test item was tested at a concentration of16 mg/L with 2 replicates corresponding to a carbon content (TOC) of10.7 mg C/L in the test vessels.The test vessels were incubated at low light conditions and at a temperature of 22 ± 2 °C.
The biodegradation of the test item was followed by titrimetric analysis of the quantity of CO2produced by the respiration of bacteria. The degradation was stopped on day 28 by acidification of the test solutions. The last titration was made on day 29 after residual CO2had been purged from the test solutions over a period of 24 hours. The percentage CO2production was calculated in relation to the theoretical CO2production (ThCO2) of the test item. The biodegradation was calculated for each titration time.
To check the activity of the test system sodium benzoate was used asfunctional control. The percentage degradation of the functional control reached the pass level of 60% within 8 days and a maximum biodegradation of 86% on day 28. In the toxicity control containing both test and reference item a biodegradation of 42% was determined within 14 days, with 42% after 28 days. The biodegradation of the reference item was not inhibited by the test itemin the toxicity control.
Both test item replicates did not reach the 10% level (beginning of biodegradation) within the 28 day period of the study. The mean biodegradation on day 28 was 3%.
Under the test conditions Octadecanoic acid, reaction products with acetic acid and tetraethylenepentamine is classified as not readily biodegradable within the 28 day period of the study.
Reference
CO2-Production and Biodegradation in the Inoculum Control and Test Item Samples
Study Day |
Date |
Inoculum |
Test Item |
||||||
Replicate 1 |
Replicate 2 |
||||||||
|
|
[mg CO2/3 L] |
[mg CO2/3 L] |
Degr. |
[mg CO2/3 L] |
Degr. |
|||
|
|
mv |
Gross |
Net Sum |
[%] |
Gross |
Net Sum |
[%] |
|
1 |
2018-06-29 |
4.4 |
5.5 |
1.1 |
1 |
5.6 |
1.2 |
1 |
|
4 |
2018-07-02 |
12.8 |
14.9 |
3.2 |
3 |
14.0 |
2.4 |
2 |
|
6 |
2018-07-04 |
16.1 |
14.4 |
3.2 |
3 |
15.3 |
2.4 |
2 |
|
8 |
2018-07-06 |
12.5 |
10.7 |
3.2 |
3 |
11.9 |
2.4 |
2 |
|
11 |
2018-07-09 |
16.0 |
13.0 |
3.2 |
3 |
15.0 |
2.4 |
2 |
|
14 |
2018-07-12 |
13.8 |
11.6 |
3.2 |
3 |
12.0 |
2.4 |
2 |
|
18 |
2018-07-16 |
16.2 |
12.2 |
3.2 |
3 |
15.1 |
2.4 |
2 |
|
21 |
2018-07-19 |
14.7 |
11.0 |
3.2 |
3 |
11.6 |
2.4 |
2 |
|
25 |
2018-07-23 |
15.5 |
12.5 |
3.2 |
3 |
11.2 |
2.4 |
2 |
|
28 |
2018-07-26 |
12.4 |
10.5 |
3.2 |
3 |
8.5 |
2.4 |
2 |
|
29* |
2018-07-27 |
8.8 |
7.8 |
3.2 |
3 |
6.2 |
2.4 |
2 |
Description of key information
Under the test conditions Octadecanoic acid, reaction products with acetic acid and tetraethylenepentamine is classified as not readily biodegradable within the 28 day period of the study.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
The ready biodegradability ofOctadecanoic acid, reaction products with acetic acid and tetraethylenepentaminewas determined with a non-adapted activated sludge over a test period of 28 days in the Modified Sturm Test. The study was conducted according to OECD 301 B. The test item was tested at a concentration of16 mg/L with 2 replicates corresponding to a carbon content (TOC) of 10.7 mg C/L in the test vessels.The test vessels were incubated at low light conditions and at a temperature of 22 ± 2 °C.
The biodegradation of the test item was followed by titrimetric analysis of the quantity of CO2produced by the respiration of bacteria. The degradation was stopped on day 28 by acidification of the test solutions. The last titration was made on day 29 after residual CO2had been purged from the test solutions over a period of 24 hours. The percentage CO2production was calculated in relation to the theoretical CO2production (ThCO2) of the test item. The biodegradation was calculated for each titration time.
To check the activity of the test system sodium benzoate was used asfunctional control. The percentage degradation of the functional control reached the pass level of 60% within 8 days and a maximum biodegradation of 86% on day 28.In the toxicity control containing both test and reference item a biodegradation of 42% was determined within 14 days, with 42% after 28 days. The biodegradation of the reference item was not inhibited by the test itemin the toxicity control.
Both test item replicates did not reach the 10% level (beginning of biodegradation) within the 28 day period of the study. The mean biodegradation on day 28 was 3%.
Under the test conditions Octadecanoic acid, reaction products with acetic acid and tetraethylenepentamine is classified as not readily biodegradable within the 28 day period of the study.
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