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EC number: 654-333-7 | CAS number: 5471-90-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
[Please provide information for all of the points below. Indicate if further information is included as attachment to the same record, or elsewhere in the dataset (insert links in 'Cross-reference' table)]
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
[Describe why the read-across can be performed (e.g. common functional group(s), common precursor(s)/breakdown product(s) or common mechanism(s) of action]
See attached documents.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
[Provide here, if relevant, additional information to that included in the Test material section of the source and target records]
See attached documents.
3. ANALOGUE APPROACH JUSTIFICATION
[Summarise here based on available experimental data how these results verify that the read-across is justified]
See attached documents.
4. DATA MATRIX
See attached documents. - Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.19 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 4.06-6.51 mg/L (95M)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.94 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: 0.75-1.23 (95%)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.3 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: and yield
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1.41 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks:
- and yield
- Details on results:
- /
- Results with reference substance (positive control):
- /
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxicity to P. subcapitata was determined using read-across from a structural analogue.
EC50 (72h - Pseudekirchneriella subcapitata) = 5.19 mg/L (growht rate) ; 0.94 mg/L (yield). - Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken from the control and each test group from the bulk test preparation at 0 hours and from the pooled replicates at 72 hours for quantitative analysis. All samples were stored frozen prior to analysis. Duplicate samples were taken at 0 and 72 hours and stored frozen for further analysis if necessary.
- Details on test solutions:
- the test item was categorized as being a ‘difficult substance’ as defined by the OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures (OECD 2000). Therefore a media preparation trial was conducted in order to determine the solubility of the test item under test conditions. This indicated that a dissolved test item concentration of approximately 68 mg/L could be obtained using a satured solution method of preparation.
A series of dilutions was made from this saturated solution to give stock solutions of 32, 10, 3.2, 1.0, 0.32 and 0.10% v/v saturated solution. An aliquot (450 mL) of each of the stock solutions was separately inoculated with 1.9 mL of algal suspension to give the required test concentrations of 0.10, 0.32, 1.0, 3.2, 10 and 32% v/v saturated solution. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Pseudokirchneriella subcapitata strain CCAP 278/4. Liquid cultures were obtained from CCAP, SAMS Research Services Ltd., Scottish Marine Institue, Oban, Argyll, Scotland. Master cultures were maintained in the laboratoty by the periodic repelenishment of culture medium under constant aeration and constant illumination at 21 +- 1 °C.
Prior to the start of the test sufficient master culture was added to approx 10 mL volumes of culture media contained in conical flasks to give an initial cell densitiy of approx 10^3 cells/ml. The flasks were plugged with polyurrethane foam stoppers and kept under constant agitation by orbital shaker (100 - 150 rpm) and constant illumination at 24 +- 1 °C until the algal density was approximately 10^4-10^5 cells/ml - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
- Post exposure observation period:
- None
- Hardness:
- no data
- Test temperature:
- 21 ± 1 °C
- pH:
- 7.3-7.7
7.8 control - Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- nominal concentrations of 0.10, 0.32, 1.0, 3.2, 10 and 32% v/v saturated solution
- Details on test conditions:
- TEST SYSTEM
- Test vessel: conical flasks plugged with polyurethan foam stoppers, kept under constant agitation by orbital shaker (100-150 rpm) and constant illumination at 24+-°C.
- Initial cells density: 1000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
NaNO3 25.5 mg/L
MgCl2.6H2O 12.16 mg/L
CaCl2.2H2O 4.41 mg/L
MgSO4.7H2O 14.6 mg/L
K2HPO4 1.044 mg/L
NaHCO3 15.0 mg/L
H3BO3 0.186 mg/L
MnCl2.4H2O 0.415 mg/L
ZnCl2 0.00327 mg/L
FeCl3.6H2O 0.160 mg/L
CoCl2.6H2O 0.00143 mg/L
Na2MoO4.2H2O 0.00726 mg/L
CuCl2.2H2O 0.000012 mg/L
Na2EDTA.2H2O 0.30 mg/L
The culture medium was prepared using reverse osmosis purified deionized water* and the pH adjusted to 7.5 with 0.1N NaOH or HCl.
OTHER TEST CONDITIONS
- Adjustment of pH: pH adjusted to 7.5 with 0.1N NaOH or HCl
- Photoperiod: constant illumination at 24+-°C
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Coulter® Multisizer Particle Counter - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 4.3-6.9 mg/L (95M)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Remarks on result:
- other: 0.79-1.3 (95%)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.32 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: and yield
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks:
- and yield
- Details on results:
- /
- Results with reference substance (positive control):
- /
- Reported statistics and error estimates:
- One way analysis of variance incorporating Bartlett's test for homogeneity of variance (Sokal and Rohlf, 1981) and Dunnett's multiple comparison procedure for comparing several treatments with a control (Dunnett, 1955) was carried out on the growth rate and yield data after 72 hours for the control and all test concentrations to determine any statistically significant differences between the test and control groups. All statistical analyses were performed using the SAS computer software package (SAS, 1999 - 2001).
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (72h - Pseudekirchneriella subcapitata) = 5.5 mg/L (growht rate) ; 1.0 mg/L (yield).
Referenceopen allclose all
Description of key information
Para-tosylcatechol (CAS: 107065-85-0) is a similar substance and the test results can be used for read-across: EC50 (72h - Pseudekirchneriella subcapitata) = 5.5 mg/L (growht rate)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 5.19 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.